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| 猪细小病毒YK株NS1基因的克隆与序列分析 | |
| 引用本文： | 白红光,卫广森,徐宏军,李尚波,王文成.猪细小病毒YK株NS1基因的克隆与序列分析[J].中国兽医科学,2005,35(8):615-619. |
| 作者姓名： | 白红光 卫广森 徐宏军 李尚波 王文成 |
| 作者单位： | 1. 沈阳农业大学,畜牧兽医学院,辽宁,沈阳,110161 2. 中国农业科学院,兰州兽医研究所,甘肃,兰州,730046 3. 辽宁益康生物制品厂,辽宁,辽阳,111000 |
| 摘 要： | 提取猪细小病毒(PPV)YK株基因组DNA,利用PCR技术扩增得到了NS1基因全序列,将其克隆到pMD18-T质粒载体并进行了序列测定和分析。结果表明,NS1基因全长1989bp,编码662个氨基酸。氨基酸序列中含有在PPV复制过程中起重要作用的保守基序GKRN,并有3个潜在的糖基化位点NISN、NFSN和NLTR。PPVYK株的NS1基因与其他PPV毒株NADL-2(4973)株、NADL-2(5075)株、NADL-2(5034)株和Kresse株相比,核苷酸同源性分别为98.3%、99.9%、99.9%和99.7%,氨基酸同源性分别为99.7%、99.7%、99.7%和97.7%。结果说明,NS1基因具有高度保守性。 |
| 关 键 词： | 猪细小病毒 NS1基因 克隆 |
| 文章编号： | 1000-6419(2005)08-0615-05 |
| 修稿时间： | 2005年4月25日 |
Cloning and sequence analysis of NS1 gene of porcine parvovirus YK strain |
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| BAI Hong-guang,WEI Guang-sen,XU Hong-jun,LI Shang-bo,WANG Wen-cheng.Cloning and sequence analysis of NS1 gene of porcine parvovirus YK strain[J].Veterinary Science in China,2005,35(8):615-619. | |
| Authors: | BAI Hong-guang WEI Guang-sen XU Hong-jun LI Shang-bo WANG Wen-cheng |
| Institution: | BAI Hong-guang~ |
| Abstract: | The genomic DNA of PPV-YK strain was extracted. The whole NS1 gene was obtained by PCR,and cloned into pMD18-T for sequence. It was found that NS1 gene of PPV-YK strain is composed of 1989 nucleotides, and encodes 662 amino acids. NS1 protein of PPV-YK strain contains the conserved sequence GKRN that is important in replication of PPV. There are three potential N-glycosylation sites NISN,NFSN and NLTR in NS1 protein of PPV-YK strain. NS1 gene of PPV-YK strain shared 98.3%,99.9%,99.9% and 99.7% identity of nucleotide sequence and 97.7%,99.7%, (99.7%) and 99.7% identity of amino acid sequence with that of NADL2(4973) strain,NADL2(5075) strain,NADL2(5034) strain and Kresse strain,respectively. The results showed that NS1 gene is highly conservative. |
| Keywords: | porcine parvovirus NS1 gene cloning |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |