| H5亚型禽流感病毒HA1基因在Sf9昆虫细胞中的表达及其抗原性检测 |
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| 引用本文： | 齐岩,孙凌霜,王彬,孙进华,王君伟,师东方.H5亚型禽流感病毒HA1基因在Sf9昆虫细胞中的表达及其抗原性检测[J].中国兽医科学,2006,36(5):352-356. |
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| 作者姓名： | 齐岩 孙凌霜 王彬 孙进华 王君伟 师东方 |
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| 作者单位： | 东北农业大学,动物医学院,黑龙江,哈尔滨,150030 |
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| 摘 要： | 利用pBlueBacHis2杆状病毒载体在昆虫细胞Sf9中表达H5亚型禽流感病毒(AIV)的 HA1基因。Western-blotting证明HA1在昆虫细胞中得到了表达,其产物具有抗原性;且不与 H7、H9 AIV的抗血清发生交叉反应,具有良好的特异性。Dot-ELISA结果显示,表达蛋白可以在非变性条件下与相应抗体作用,同样具有良好的抗原性。试验结果证明,表达的H5亚型AIV的 HA1蛋白,为检测禽类体内是否感染H5亚型AIV和监测免疫禽类血清H5抗体水平提供了优良的检测抗原。
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| 关 键 词： | 禽流感病毒 HA1基因 杆状病毒 真核表达 |
| 文章编号： | 1673-4696(2006)05-0352-05 |
| 修稿时间： | 2006年1月16日 |
Eukaryotic expression of HA1 gene of H5 avian influenza virus in insect cells and the antigenicity of the expressed protein |
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| QI Yan,SUN Ling-shuang,WANG Bin,SUN Jin-hua,WANG Jun-wei,SHI Dong-fang.Eukaryotic expression of HA1 gene of H5 avian influenza virus in insect cells and the antigenicity of the expressed protein[J].Veterinary Science in China,2006,36(5):352-356. |
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| Authors: | QI Yan SUN Ling-shuang WANG Bin SUN Jin-hua WANG Jun-wei SHI Dong-fang |
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| Abstract: | HA1 gene of H5 avian influenza virus(AIV) was expressed in insect cells by pBlueBacHis2 baculovirus vector. Western-blotting analysis showed that the expressed HA1 protein had antigenicity and did not react with H7 and H9 AIVs anti-serum. Dot-ELISA analysis showed that the expressed recombi-nant protein had good and special characteristics under non-denaturing conditions. These results demonstrated that the expressed HA1 protein of H5 AIV was a good antigen for detection of H5 AIV infection and for determination of antibody level in vaccinated avian. |
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| Keywords: | avian influenza virus HA1 gene baculovirus eukaryotic expression |
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