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| 抗O型口蹄疫病毒单克隆抗体的制备及其生物学特性分析 | |
| 引用本文： | 林彤,杜惠芬,吴海波,邵军军,独军政,丛国正,常惠芸.抗O型口蹄疫病毒单克隆抗体的制备及其生物学特性分析[J].中国兽医科学,2007,37(3):227-231. |
| 作者姓名： | 林彤 杜惠芬 吴海波 邵军军 独军政 丛国正 常惠芸 |
| 作者单位： | 1. 甘肃农业大学,动物医学院,甘肃,兰州,730070;中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,甘肃,兰州,730046 2. 甘肃省医学科学院,医学生物技术中心,甘肃,兰州,730050 3. 浙江省医学科学院,生物工程研究所,浙江,杭州,310013 4. 中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,甘肃,兰州,730046 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 用灭活O型口蹄疫病毒为抗原免疫BALB/c小鼠4次后,取致敏的脾B淋巴细胞和SP2/0小鼠骨髓瘤细胞进行融合,在HAT选择培养基中培养2周,经间接ELISA法筛选,最终获得了3株抗O型FMDV的阳性杂交瘤细胞株,命名为2F1、2G7和6H4。血清学试验证明该McAbs能与FMDV抗原结合,具有高度特异性。 |
| 关 键 词： | 口蹄疫病毒 单克隆抗体 生物学特性 |
| 文章编号： | 1673-4696(2007)03-0227-05 |
| 修稿时间： | 2006年10月20 |
Development of monoclonal antibodies against foot-and-mouth disease virus O type and analysis of their biological characteristics |
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| LIN Tong,DU Hui-fen,WU Hai-bo,SHAO Jun-jun,DU Jun-zheng,CONG Guo-zheng,CHANG Hui-yun.Development of monoclonal antibodies against foot-and-mouth disease virus O type and analysis of their biological characteristics[J].Veterinary Science in China,2007,37(3):227-231. | |
| Authors: | LIN Tong DU Hui-fen WU Hai-bo SHAO Jun-jun DU Jun-zheng CONG Guo-zheng CHANG Hui-yun |
| Abstract: | To obtain monoclonal antibodies(McAbs) against foot-and-mouth disease virus(FMDV) O type,BALB/c mice were immunized with attenuated FMDV O type,and then lymphocytes from the sple-nic tissues of the mice were fused with SP2/0 myeloma cell.By limiting dilution,three monoclonal antibo-dies(McAbs) against FMDV named 2F1,6H4 and 2G7 were screened by the indirect ELISA.These McAbs could specifically recognize FMDV O type antigens in a serological test. |
| Keywords: | foot-and-mouth disease virus monoclonal antibodies biological characteristic |
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