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| 猪胸膜肺炎放线杆菌外膜脂蛋白基因的克隆及序列分析 | |
| 引用本文： | 杨学山,逯忠新,胡永浩,赵卫平,赵萍,高鹏程,储岳峰.猪胸膜肺炎放线杆菌外膜脂蛋白基因的克隆及序列分析[J].中国兽医科学,2004,34(2):17-20. |
| 作者姓名： | 杨学山 逯忠新 胡永浩 赵卫平 赵萍 高鹏程 储岳峰 |
| 作者单位： | 1. 甘肃农业大学,动物医学院,甘肃,兰州,730070;中国农业科学院兰州兽医研究所,甘肃,兰州,730046 2. 中国农业科学院兰州兽医研究所,甘肃,兰州,730046 3. 甘肃农业大学,动物医学院,甘肃,兰州,730070 |
| 摘 要： | 以国内主要流行的猪胸膜肺炎放线杆菌 (APP)QH 1、HN 7菌株的基因组DNA为模板 ,通过PCR方法扩增出外膜脂蛋白 (OML)基因片段 ,然后将其克隆至 pMD18 T载体中 ,经酶切和PCR鉴定 ,对阳性克隆进行序列测定。将测序结果分别与标准菌株进行比较 ,QH 1株的核苷酸序列与血清 1、9、11、12型参考株的同源性达 99.1%～ 99.9% ;HN 7株的核苷酸序列与血清 7、3、4、6型参考株的同源性达 97.3%～ 10 0 % ,与其他血清型参考株的同源性较低。 |
| 关 键 词： | 胸膜肺炎放线杆菌 外膜脂蛋白基因 克隆 序列分析 |
| 文章编号： | 1000-6419(2004)02-0017-04 |
| 修稿时间： | 2003年11月14 |
Cloning and sequence analysis of gene encoding outer membrane lipoprotein of Actinobacillus pleuropneumoniae in swine |
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| YANG Xue-shan.Cloning and sequence analysis of gene encoding outer membrane lipoprotein of Actinobacillus pleuropneumoniae in swine[J].Veterinary Science in China,2004,34(2):17-20. | |
| Authors: | YANG Xue-shan |
| Institution: | YANG Xue-shan~ |
| Abstract: | The DNA was abstracted fromActinobacillus pleuropneumoniae (APP) QH-1 strain and HN-7 strain that are predominantly prevalent strains in China. The fragment of the outer membrane lipoprotein (OML) gene of A.pleuropneumoniae was amplified by polymerase chain reaction (PCR) and cloned into the pMD18-T vector. Positive clones of OML gene were identified by restriction analysis and PCR, and then sequenced. The result of sequence analysis for theORF of OML gene indicated that the nucleotide sequence of QH-1 strain shared 99.1%-100% homologywith that of the reference serotype 1,9,11 and 12, but the nucleotide sequence of HN-7 strain shared 97.3%-99.9% homologywith that of the reference serotype 3,4,6 and 7. The nucleotide sequence of QH-1 strain and HN-7 strain exhibited low homologywith that of other reference strains. |
| Keywords: | Actinobacillus pleuropneumonia OML gene cloning sequence analysis |
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