| 鹅细小病毒vp1基因的原核表达及抗原性分析 |
| |
| 引用本文： | 马波,于天飞,李俚,布日额,高明春,王君伟.鹅细小病毒vp1基因的原核表达及抗原性分析[J].中国兽医科学,2010,40(2). |
| |
| 作者姓名： | 马波 于天飞 李俚 布日额 高明春 王君伟 |
| |
| 作者单位： | 马波,李俚,高明春,王君伟(东北农业大学,动物医学院,预防兽医系,黑龙江,哈尔滨,150030);于天飞(齐齐哈尔大学,生命学院,黑龙江齐齐哈尔,161006);布日额(内蒙古民族大学,生命科学学院,内蒙古,通辽,028043) |
| |
| 基金项目： | 黑龙江省教育厅重大科技项目，黑龙江省科技攻关项目 |
| |
| 摘 要： | 将鹅细小病毒(GPV)H1株vp1基因从pMD18T-vp1亚克隆至原核表达载体pGEX-6P-1的多克隆位点,构建了原核表达载体pGEX-6P-vp1,将其转化感受态细胞BL21(DE3)pLysS中,经IPTG诱导,SDS-PAGE分析,结果获得了108ku的融合蛋白,该融合蛋白经亲和层析纯化并经Western-blot检测。结果表明,纯化的融合蛋白可与GPV阳性血清发生特异性反应,具有良好的抗原性。
|
| 关 键 词： | 鹅细小病毒 vp1基因 原核表达 抗原性 |
Prokaryotic expression of vp1 gene of goose parvovirus and antigenicity analysis of the expressed protein |
| |
| MA Bo,YU Tian-fei,LI Li,BU Ri-e,GAO Ming-chun,WANG Jun-wei.Prokaryotic expression of vp1 gene of goose parvovirus and antigenicity analysis of the expressed protein[J].Veterinary Science in China,2010,40(2). |
| |
| Authors: | MA Bo YU Tian-fei LI Li BU Ri-e GAO Ming-chun WANG Jun-wei |
| |
| Institution: | MA Bo1,YU Tian-fei2,LI Li1,BU Ri-e3,GAO Ming-chun1,WANG Jun-wei1(1.Department of Preventive Veterinary Medicine,College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China,2.College of Life Science , Engineering,Qiqihar University,Qiqihar 161006,3.College of Life Sciences,Inner Mongolia University for Nationalities,Tongliao 028043,China) |
| |
| Abstract: | A vp1 gene of goose parvovirus(GPV) H1 strain was cloned from the pMD18T-vp1 into the polycloning sites of pGEX-6P-1 vector.The recombinant prokaryotic expression plasmid was constructed,and named pGEX-6P-vp1.The recombinant plasmid was transformed into BL21(DE3) pLysS.The transformed bacteria were induced with IPTG and the expressed protein was analyzed by SDS-PAGE.The fusion protein of 108 ku was expressed.The protein purified by chromatography purification system could react with GPV positive serum speci... |
| |
| Keywords: | goose parvovirus vp1 gene prokaryotic expression antigenicity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
