死后不同时间的组织细胞DNA含量分析

本文应用流式细胞仪对大鼠死亡后不同时间的心、肝、肾组织细胞DNA含量进行了分析。结果显示:与死后0点细胞DNA含量值相比,6小时细胞DNA含量下降到99.5％,12小时为91.3％,18小时为87.1％,24小时为81.3％,30小时为76.7％,36小时为74.3％,48小时为72.3％。上述规律性变化,将可能对早期死亡时间的推断提供客观可靠的依据。     

Pathways to early delinquency: Exploring the individual and collective contributions of difficult temperament,low maternal involvement,and externalizing behavior

BackgroundThe purpose of this study was to investigate the impact of difficult temperament and maternal involvement, measured at ages one and three years, respectively, on externalizing behavior at age five and early delinquency at age nine.MethodsMaternal- and child-reports from 4,897 members of the Fragile Families and Child Wellbeing Study (FFCWS) were included in a path analysis of four of five waves of FFCWS data: Wave 2 (difficult temperament at age 1), Wave 3 (maternal involvement at age 3), Wave 4 (externalizing behavior at age 5), and Wave 5 (delinquency at age 9).FindingsAlthough difficult temperament at age 1 displayed a weak zero-order correlation with delinquency at age 9 and low maternal involvement at age 3 failed to correlate with delinquency at age 9, both entered into significant chained relationships with delinquency via externalizing behavior at age 5. In addition, difficult temperament at age 1 seemed to evoke low parental involvement at age 3.ConclusionsThe respective roles of a difficult temperament, maternal involvement, and externalizing behavior in a proximal chaining process may be partially responsible for the continuity that has been observed in antisocial behavior over time.     

Deaths among narcotic addicts in Denmark in 1978 and 1979

Objective measurements were carried out to study the evolution of rigor mortis on rats at various temperatures.Our experiments showed that:(1) at 6 °C rigor mortis reaches full development between 48 and 60 hours post mortem, and is resolved at 168 hours post mortem; (2) at 24 °C rigor mortis reaches full development at 5 hours post mortem, and is resolved at 16 hours post mortem; (3) at 37 °C rigor mortis reaches full development at 3 hours post mortem, and is resolved at 6 hours post mortem; (4) the intensity of rigor mortis grows with increase in temperature (difference between values obtained at 24 °C and 37 °C); and (5) at 6 °C a “cold rigidity” was found, in addition to and independent of rigor mortis.     

实验性小白鼠死后肝脏酶组织化学改变

作者用60只小鼠实验,分组观察环境温度为4℃和20℃、死后0、6、12、24、48和72h 肝脏的PPr、ATPase、CCO、ACP、ANAE、G-6-Pase、LDH、SDH、G6PD 和NADHD 等10种酶活性的组织化学改变。在4℃组,PPr 活性在死后6h 开始下降;死后72h,PPr 仅在少数肝细胞呈阳性反应;其余9种酶活性无明显改变。在20℃组,死后6h,PPr 活性明显下降,死后12h 变为阴性。死后48h,LDH 和ATPase 活性明显下降,死后72h 变为阴性。作者认为,死后组织中酶的改变规律有助于推断死后经过时间。     

面向海洋世纪 确立海法研究体系

海上活动的多样化和复杂化以及海洋经济的蓬勃发展,突出了海法研究体系的重要地位。在分析海法的概念、所调整的社会关系、确立海法体系的必要性的基础上,将海法研究体系归纳为三个层次,并分别进行探讨。     

脑干损伤后神经元及轴突改变的组织化学观察

采用针刺法造成大鼠脑干损伤,用尼氏体染色、嗜银染色和改良三色法观察脑干神经元及轴突在伤后不同时间的病理改变。结果发现,嗜银染色显示伤后 1～ 3h部分神经纤维不规则增粗、少数断裂, 6h断端膨大呈球形, 15h收缩球较为明显,至 24h收缩球明显且数量增多;改良三色法显示伤后 3～ 6h部分髓鞘与轴突之间的间隙增宽, 15h髓鞘明显弯曲、不完全地附着在轴突表面,甚至剥脱,持续到伤后 24h;尼氏体染色显示神经元核周尼氏体在伤后 24h减少。该结果提示,组织化学染色能观察到脑干损伤后的病理改变,并且有可能用于推断脑干损伤时间。     

我国海上犯罪体系亟需健全与完善

我国海上犯罪体系指由国际刑法规范和我国刑法规范所调整的各种具体涉海犯罪构成的统一体。我国现行海上犯罪体系存在诸多缺陷和不足,难以适应我国实施海洋发展战略、履行国际条约义务和维护我国海洋权益之需要,亟需健全与完善。     

大鼠脑挫伤后脑组织β-APP表达与损伤时间的关系

目的观察大鼠实验性脑挫伤后不同时间内脑组织β淀粉样前体蛋白（β-APP）表达的变化,探讨β-APP与脑损伤经过时间的关系。方法参照Feeney’s法建立大鼠脑挫伤模型,在伤后1h,4h,12h,48h,72h,7d,14d,运用免疫组化SABC法和Westernblot法检测β-APP的表达,以非损伤组做对照。结果β-APP出现于损伤后1h,4h开始增加至12h达到高峰,随后阳性反应细胞逐渐减少,7d后仍有少量表达但仍高于对照组,14d后表达基本恢复至接近对照组水平,各实验组与对照组比较有统计学意义（P〈0.05）。结论β-APP在脑挫伤后随时间的变化,其表达呈现先逐渐增加后又减少的一定规律性变化。     

Mitochondrial DNA and STR analyses of maggot crop contents: effect of specimen preservation technique

DNA analysis of maggot crop contents can be used to identify a missing body or aid entomologists with interpreting evidence used for PMI estimations. Entomological evidence is often collected and preserved to keep identifiable external features intact. The preservation methods currently in use may not be suitable for preserving DNA in the maggot crop for later analysis. In this study, carrion maggots raised on human tissue were preserved under the following 8 preservation conditions: no fluid at -70 degrees C, no fluid at 4 degrees C, no fluid at 24 degrees C, 70% ethanol at 4 degrees C, 70% ethanol at 24 degrees C, 95% ethanol at 24 degrees C, Kahle's solution at 24 degrees C and formaldehyde at 24 degrees C. Maggots were dissected following 2 weeks, 8 weeks and 6 months of preservation. The maggot crops were extracted, human DNA was quantitated, and an attempt was made at amplifying mitochondrial DNA (mtDNA) and short tandem repeat (STR) loci. Both mtDNA and STRs were successfully amplified from maggots stored in ethanol or without any preservation fluid. Formalin-containing preservation solutions reduced the recovery of DNA. The best results were observed from maggots stored without any preservation fluid at -70 degrees C.     

Long-term storage of blood samples as whole blood at extremely low temperatures for methemoglobin determination

Changes in methemoglobin (Met-Hb) concentrations during storage of whole blood and a hemolysate at refrigerated or various freezing temperatures were examined using experimentally prepared blood samples. When whole blood was stored at 3 degrees C, rapid reduction of Met-Hb was observed in the nitrite-treated blood whereas neither reduction nor formation of Met-Hb was observed in the untreated and heated blood within 7 days. When hemolysate was stored at 3 degrees C, Met-Hb concentrations were stable within a few days regardless of the initial values. However, slight autoxidation was observed 7 days after storage in the untreated and heated blood. When whole blood was stored at various freezing temperatures, Met-Hb concentrations were practically stable until at least 30 days at -80 degrees C or -196 degrees C regardless of the initial values, although considerable autoxidation was observed at -30 degrees C especially in the blood containing small amounts of Met-Hb. Based on the results obtained, a new method was devised for long-term storage of whole blood at extremely low temperatures for Met-Hb determinations.     

Marriage in urban Albania (during the first half of the twentieth century)

This article analyses the marriage pattern in urban Albania, based primarily on the data of the Albanian Population Census of 1918. Age at marriage and the factors influencing nuptiality among the urban population are analysed using a combination of quantitative and qualitative data. The analyses show that the marriage pattern in urban Albania was similar to Laslett's Mediterranean set, while rural areas displayed an age at marriage for both men and women which was characteristic of the marriage pattern East of the Hajnal line. Albanian cities showed a higher age at marriage for both men and women than villages. The difference in age at marriage between urban and rural areas was noticeably higher for men, while the age at marriage for women showed smaller differences. An explanation for these differences in the age at marriage was found in a combination of traditional marital behaviour and demographic issues, which broadened or narrowed the marriage field, thus directly influencing the age at marriage.     

Fluorescence spectra and images of latent fingerprints excited with a tunable laser in the ultraviolet region

Fluorescence spectra of sebum-rich latent fingerprints were studied with a tunable laser for non-destructive fingerprint detection without chemical treatment. The tunable laser consists of a nanosecond pulsed Nd-YAG laser and an optical parametric oscillator (OPO) crystal. The fluorescence spectra and images were measured at various excitation wavelengths in the ultraviolet region by the time-resolved fluorescence method. We have previously reported that a typical fluorescence spectrum of fingerprints consists of two peaks located at c. 330 and 440 nm. In order to determine the wavelength of optimal excitation, excitation spectra were measured at wavelengths ranging from 220 to 310 nm. The fluorescence intensity of the 330 nm peak became maximal with excitation at 280 nm. The images of latent fingerprints on white papers were also measured and the clearest image was obtained with excitation at 280 nm. The influence of continuous irradiation on the fluorescence of fingerprints was measured at the optimal excitation wavelengths. The 330 nm peak was strong at first and decreased with continuous irradiation, whereas the 440 nm peak, which was weak at first, increased gradually.     

大陆法系各国（地区）亲告罪立法之比较

告诉乃论制度对充分保障被害人权利、实现刑罚谦抑、促进刑事和解具有重要意义。大陆法系各国均规定了数量庞大的亲告罪,我国的亲告罪名却很少,类型也很单一。制约亲告罪范围扩大的真正原因在于我国亲告罪告诉机制的缺陷。我国刑法与刑事诉讼法对亲告罪告诉权的救济性规则缺乏,亲告罪与自诉罪混淆,告诉权与证明责任混淆,告诉制度不完善。通过归纳、比较各国刑事立法例中的优劣,认为我国可采被害人可自由选择自诉的混合起诉制,以弥补亲告罪告诉机制的缺陷。     

基层领导干部依法行政能力现状与分析

通过对基层领导干部依法行政能力的调查和分析,概括了基层领导干部在依法行政方面的现状和特点,分析了基层领导干部在依法行政方面存在的问题,并对如何提高基层领导干部依法行政能力提出了若干对策。     

The effects of cognitive-behavioral treatment for forensic inpatients

The effects of an intramural cognitive-behavioral treatment for forensic inpatients with personality disorders in a high-security hospital were examined. Treatment was aimed at modifying maladaptive coping and social skills, at enhancing social awareness, at reducing egoistic and oppositional behaviors, and at reducing psychological complaints. The patients, who all had committed serious crimes (violence, arson, sexual offences), participated voluntarily in the study. A total of 39 patients started the study, but during the course of the study, several patients dropped out because of several reasons. Patients as a group showed significant improvements over time on psychopathological symptoms, personality traits, and coping. A significant decrease of oppositional behaviors was reported by the staff. Though the patients improved well at the group level, only a minority of patients showed reliable change over time at the individual level. The meaning of the results in relation to treatment are discussed.     

大鼠弥散性轴索损伤后β-APP的表达

目的观察大鼠DAI损伤后β-APP表达和Gless神经纤维轴索染色在诊断DAI损伤及判断损伤时间的价值。方法按Marmarou法复制大鼠DAI损伤模型,脑组织常规取材后进行β-APP及Gless氏神经纤维轴索染色观察。结果β-APP及Gless氏染色法在大鼠DAI损伤后0.5h即可见神经轴索断裂、扭曲变形、增粗膨大,12h以后可见到轴索收缩球。二种方法均显示DAI损伤的病理形态学变化,伤后12h明显,1d达到高峰,3d后开始修复,10d后基本恢复正常。β-APP表达强度在实验组不同时间存在着明显的差异,即3h呈明显阳性表达,1d达到高峰,3d后逐渐减弱,10d基本恢复正常。结论β-APP免疫组织化学染色法及Gless氏神经纤维轴索染色法,对DAI的早期诊断具有重要应用价值,并能从病理形态学上反映DAI损伤的时序性。β-APP表达强度变化是推断早期DAI损伤时间的重要参考指标。     

Marriage in urban Albania (during the first half of the twentieth century)

This article analyses the marriage pattern in urban Albania, based primarily on the data of the Albanian Population Census of 1918. Age at marriage and the factors influencing nuptiality among the urban population are analysed using a combination of quantitative and qualitative data. The analyses show that the marriage pattern in urban Albania was similar to Laslett's Mediterranean set, while rural areas displayed an age at marriage for both men and women which was characteristic of the marriage pattern East of the Hajnal line. Albanian cities showed a higher age at marriage for both men and women than villages. The difference in age at marriage between urban and rural areas was noticeably higher for men, while the age at marriage for women showed smaller differences. An explanation for these differences in the age at marriage was found in a combination of traditional marital behaviour and demographic issues, which broadened or narrowed the marriage field, thus directly influencing the age at marriage.     

不同条件下Percoll密度梯度离心分离组织细胞浮游生物的比较

目的实验评估不同条件下Percoll密度梯度离心分离组织中浮游生物的效果。方法大白兔肝组织匀浆后与3种藻类混合物混匀,分为混匀高速分离组、混匀低速分离组、叠加高速分离组、叠加低速分离组和未分离组(对照组)进行Percoll密度梯度离心分离浮游生物,镜检并提取其DNA,用PCR技术分别检测浮游生物16S rDNA和叶绿素基因特异性片段。结果混匀高速分离组有较多浮游生物呈分层条纹状分布于离心管中部,叠加高速分离组浮游生物紧邻组织细胞层下,低速分离组见较少浮游生物紧邻于组织细胞层之下,未分离组见浮游生物和组织细胞混杂沉于管底;经DNA检测,各分离组组织细胞层下液体均可检出浮游生物的1条447bp 16S rDNA片段及1条194bp叶绿体/叶绿素脱辅基蛋白基因片段,未分离组均未检出扩增产物且背景较深。结论混匀加样方式进行高速Percoll密度梯度离心,合并收集组织细胞层下所有液体,是分离浮游生物的最有效方法。     

Coping,confidence, and change within the academy: a longitudinal look at police recruits

This study is based on surveys of police recruits representing four agencies across the United States. The recruits were surveyed at the beginning and end of their academy training and asked about coping strategies and the confidence they had in performing their jobs. Coping shifted significantly over time, with recruits utilizing task-oriented and outreach strategies less frequently at the end of the academy than at the beginning. Avoidance coping strategies were used more frequently by recruits at the end of the academy than at the beginning. Slight changes were also found in the influence of these strategies on job confidence over time, with avoidance coping having a stronger influence in the beginning of the academy than at the end. The role of demographic factors on coping was largely invariant over time, with only slight differences detected. Implications for policy and research are also discussed.     

Blood, bone marrow and eye fluid ethanol concentrations in putrefied rabbits

The effect of putrefaction on postmortem blood, bone marrow and eye fluid ethanol levels was evaluated in rabbits. Control and dosed animals were sacrificed and stored at either room temperature (approx. 19 degrees C) or cold temperature (approx. 3.5 degrees C) for as long as 28 days. Control animals stored at room temperature showed ethanol levels in the bone marrow that peaked at 7 days after sacrifice, followed by decreases to a nondetectable level at 21 days. Overall decreases were demonstrated in bone marrow of dosed rabbits stored at room temperature for all postmortem intervals. The control animals stored at low temperature showed no ethanol in the bone marrow and blood until 21 days after sacrifice. Dosed rabbits stored at low temperature showed no significant changes in blood and marrow ethanol until 21 days after sacrifice.