猪白细胞介素21基因的原核表达与多抗血清的制备

为了制备猪白细胞介素21(porcine interleukin-21,pIL-21)的多克隆抗体,采用RT-PCR从猪外周血单个核细胞(PBMC)扩增获得pIL-21基因,然后将其克隆至大肠杆菌表达载体pET-32a(+)之中,转化至大肠杆菌BL21(DE3),用IPTG诱导表达。SDS-PAGE和Western-blot分析结果显示,pIL-21以可溶性蛋白形式表达,分子质量为35ku,具有良好的反应原性。经过Ni-NTA镍离子亲和层析纯化,获得纯化的重组蛋白pIL-21。用其免疫BALB/c小鼠制备抗pIL-21血清抗体。Western-blot分析结果显示,该抗体可与杆状病毒表达的重组Cap-pIL-21、ConA和PHA共刺激的猪PBMC细胞发生特异性反应,ELISA抗体效价可达1∶12 800。上述研究结果为pIL-21的生物学功能研究奠定了重要基础。     

Thinking on the Theory of "China’s Century"

As China’s economy and global influence increase,its international relations are quickly changing.As more credence is given to predictions of a"China Century"to follow the"American Century,"interest is also increasing in the adjustments China is making to its strategic diplomacy as it prepares to take the first chair from the United States.The conceptual innovation in China’s diplomacy in 2014 is a significant harbinger in the eyes of many as to how this China Century will shape up.While China’s new diplomacy is praised,opinion differs on its future intentions.After a series of proposals were put forward at summit meetings of CICA(Conference on Interaction and Confidence-Building Measures in     

Analysis of Forensic Autopsy in 120 Cases of Medical Disputes Among Different Levels of Institutional Settings

Despite advances in medical science, the causes of death can sometimes only be determined by pathologists after a complete autopsy. Few studies have investigated the importance of forensic autopsy in medically disputed cases among different levels of institutional settings. Our study aimed to analyze forensic autopsy in 120 cases of medical disputes among five levels of institutional settings between 2001 and 2012 in Wenzhou, China. The results showed an overall concordance rate of 55%. Of the 39% of clinically missed diagnosis, cardiovascular pathology comprises 55.32%, while respiratory pathology accounts for the remaining 44. 68%. Factors that increase the likelihood of missed diagnoses were private clinics, community settings, and county hospitals. These results support that autopsy remains an important tool in establishing causes of death in medically disputed case, which may directly determine or exclude the fault of medical care and therefore in helping in resolving these cases.     

中国道路：中国梦的实现途径

党的十八大指出：道路关乎国家前途、民族命运、人民幸福,实现中国梦必须走中国道路。中国道路的成功开辟为实现中国梦创造了条件和途径。中国梦内涵丰富,凝聚了近代以来无数中华儿女为实现国家富强、民族复兴和人民幸福的伟大梦想。中国道路和中国梦二者具有高度的内在一致性,中国梦的实现必然要求坚持走中国道路,而坚持走中国道路则是中国梦实现的根本途径。     

＂Quasi-Cold War＂ between China and the U.S.： Characteristics, Causes and China＇s Response

Sino-U.S. relations are moving toward "Quasi-Cold War." Various factors contribute to Sino-U.S. "Quasi-Cold War". China should actively deal with this kind of situation, which will continue in the future, to prevent "Quasi-Cold War" sliding into a new "Cold War."     

H19基因上游差异性甲基化区SNPs多态性研究

目的获得H19基因上游差异性甲基化区中SNPs的群体遗传学信息。方法采用PCR和测序技术,对105例中国北方汉族健康无关个体H19上游启动子区检测;使用Haploview 4.1和PowerStats V12软件进行统计学分析。选用甲基化敏感的限制内切酶(msRE)HpaⅡ,检测5个家系样本H19等位基因的亲代来源。结果测序结果显示,H19启动子区含有13个SNPs,组成5种单倍型,13种单倍型组合,其个体识别能力为0.856、多态性信息含量为0.67、非父排除率为0.498。经msRE HpaⅡ消化母源等位基因后,进行PCR及测序分析,检测出父源等位基因,排除1例和肯定4例家系的亲缘关系。结论 DNA甲基化标记和SNPs多态性检测,可同时进行多态性分型并确定等位基因的亲代来源,具有较高的法医学应用价值。     

大鼠弥漫性轴索损伤脑组织弥散张量成像与免疫组化染色观察

目的观察大鼠弥漫性轴索损伤(DAI)模型伤后不同时间大脑白质磁共振弥散张量成像(DTI)弥散参数和β-APP、NF68免疫组织化学染色的变化。方法建立SD大鼠Marmarou DAI模型,分别于伤后3、12、24、72 h行DTI扫描,获取大鼠胼胝体、内囊和外囊弥散参数;比较相应部位β-APP单位面积阳性轴索数及阳性染色面积百分比,NF68平均光密度的伤后变化。结果实验组胼胝体、内囊和外囊轴向弥散(AD)、各向异性分数(FA)伤后逐步下降;β-APP单位面积阳性轴索数量、阳性染色面积百分比及NF68平均光密度伤后逐步增加。相关分析证实AD、FA值与β-APP、NF68变化呈显著负相关。结论 DTI弥散参数可作为DAI早期诊断的生物标记物。     

DRD5基因遗传多态性与精神状态的相关性及法医学意义

多巴胺是人类神经系统中重要的神经递质,通过与受体结合发挥生物学作用。当编码受体的基因异常时,可能导致精神疾病的发生。多巴胺受体有5种类型,分别为DRD1、DRD2、DRD3、DRD4、DRD5。DRD5是精神疾病的一个重要候选基因。DRD5受体基因上的rs7655090、rs1850744、(TC)n与精神疾病可能相关联;同时也存在矛盾的研究结果,表明精神疾病可能为多因素、多位点、多基因复制遗传疾病。DRD5基因的SNP具有较好的遗传多态性,可为法医学个人识别与亲权鉴定提供新的遗传标记及为司法精神病鉴定提供参考性遗传学指标。     

判决书“附带”:以中国基层司法“法官后语”实践为主线

判决书"附带",尤其是中国基层司法实践中的"法官后语",折射了关于判决书定义、功能,及法官角色和更宏大的司法的理论复杂,需深入辨析。而历史化地理解问题,并引入微观司法需求互动和宏观社会需求互动的概念,可深入洞察其关键,并获得关于判决书"附带"至司法等系列问题的"社会约定"的思考路径。由此推进,重视并积极回应被司法者和广泛司法关注者对司法的愿景,符合司法政治学的正当性理念,亦对中国现代正规司法建设有益。     

美国人力资源战略的实施策略及对我国的启示

美国人力资源战略的成功实施使其在世界人才竞争中一直保持竞争优势,这与其选择合理的实施策略是分不开的。本文通过梳理美国国家层面人力资源战略的相关政策和措施,归纳了美国实施人力资源战略的主要策略选择,提出对我国实施人力资源战略实施的一些启示。