Allele frequencies of 15 STR loci in the population of the city of Quito, Ecuador

POPULATION: Quito City population (Ecuador, South America, n = 116-207).     

An updated validation of Promega's PowerPlex 16 System: high throughput databasing under reduced PCR volume conditions on Applied Biosystem's 96 capillary 3730xl DNA Analyzer

The PowerPlex 16 System from Promega Corporation allows single tube multiplex amplification of sixteen short tandem repeat (STR) loci including all 13 core combined DNA index system STRs. This report presents an updated validation of the PowerPlex 16 System on Applied Biosystem's 96 capillary 3730xl DNA Analyzer. The validation protocol developed in our laboratory allows for the analysis of 1536 loci (96 x 16) in c. 50 min. We have further optimized the assay by decreasing the reaction volume to one-quarter that recommended by the manufacturer thereby substantially reducing the total cost per sample without compromising reproducibility or specificity. This reduction in reaction volume has the ancillary benefit of dramatically increasing the sensitivity of the assay allowing for accurate analysis of lower quantities of DNA. Due to its substantially increased throughput capability, this extended validation of the PowerPlex 16 System should be useful in reducing the backlog of unanalyzed DNA samples currently facing public DNA forensic laboratories.     

AMPFlSTR Identifiler STR allele frequencies in Tanzania, Africa

POPULATION:  Identifiler—Employees and students of Muhimibili University College of Health Sciences in Dar es Salaam representing 19 widely distributed administrative districts and 42 tribes within the country.     

A forensic laboratory tests the Berkeley microfabricated capillary array electrophoresis device

Miniaturization of capillary electrophoresis onto a microchip for forensic short tandem repeat analysis is the initial step in the process of producing a fully integrated and automated analysis system. A prototype of the Berkeley microfabricated capillary array electrophoresis device was installed at the Virginia Department of Forensic Science for testing. Instrument performance was verified by PowerPlex 16 System profiling of single source, sensitivity series, mixture, and casework samples. Mock sexual assault samples were successfully analyzed using the PowerPlex Y System. Resolution was assessed using the TH01, CSF1PO, TPOX, and Amelogenin loci and demonstrated to be comparable with commercial systems along with the instrument precision. Successful replacement of the Hjerten capillary coating method with a dynamic coating polymer was performed. The accurate and rapid typing of forensic samples demonstrates the successful technology transfer of this device into a practitioner laboratory and its potential for advancing high-throughput forensic typing.     

Development and validation of the AmpFlSTR MiniFiler PCR Amplification Kit: a MiniSTR multiplex for the analysis of degraded and/or PCR inhibited DNA

DNA typing of degraded DNA samples can be a challenging task when using the current commercially available multiplex short tandem repeat (STR) analysis kits. However, the ability to type degraded DNA specimens improves by redesigning current STR marker amplicons such that smaller sized polymerase chain reaction (PCR) products are generated. In an effort to increase the amount of information derived from these types of DNA samples, the AmpFlSTR MiniFiler PCR Amplification Kit has been developed. The kit contains reagents for the amplification of eight miniSTRs which are the largest sized loci in the AmpFlSTR Identifiler PCR Amplification Kit (D7S820, D13S317, D16S539, D21S11, D2S1338, D18S51, CSF1PO, and FGA). Five of these STR loci (D16S539, D21S11, D2S1338, D18S51, and FGA) also are some of the largest loci in the AmpFlSTR SGM Plus kit. This informative nine-locus multiplex, which includes the gender-identification locus Amelogenin, has been validated according to the FBI/National Standards and SWGDAM guidelines. Our results demonstrate significant performance improvements in models of DNA degradation, PCR inhibition, and nonprobative samples when compared to the AmpFlSTR Identifiler and SGM Plus kits. These data support that the MiniFiler kit will increase the likelihood of obtaining additional STR information from forensic samples in situations in which standard STR chemistries fail to produce complete profiles.     

Subsumtionsautomat oder Ersatzgesetzgeber?

Der Beitrag besch?ftigt sich mit jener Rolle, die der Rechtsprechung bezüglich der Weiterentwicklung des ?sterreichischen Wohnrechts zukommt. Er zeigt ua die praktische Bedeutung auf, die der richterlichen Rechtsanwendung und Rechtsfortbildung in diesem Bereich zukommt, thematisiert die Frage, ob es in der wohnrechtlichen Rechtsprechung zu "Grenzverwischungen" zwischen Rechtsanwendung und Rechtspolitik kommt und analysiert charakteristische St?rken und Schw?chen von richterlicher Rechtsfortbildung im Wohnrecht.     

十六届中共中央政治局集体学习综论

十六届中共中央政治局在继承和发扬党的三代中央领导集体重视学习、善于学习的优良传统、理论联系实际的优良学风的基础上,一个突出的特点是中央政治局集体学习的制度化。44次集体学习是围绕着全面建设小康社会这个中心任务展开的。这些重视学习、加强学习的安排,带着战略眼光、历史眼光、世界眼光来学习的方法,对建设学习型政党发挥着重要的领导和示范作用。     

载脂蛋白J与脑损伤的研究进展

载脂蛋白J在脑损伤中发挥重要的神经保护功能,参与脑损伤修复,在损伤后不同时段,Apo J含量及定位均呈现规律性的波动,即时空动态性,因此,对脑损伤的治疗及法医病理学脑损伤时间推断具有重要意义,本文综述了近期Apo J相关研究进展。     

用市场化手段确立按贡献分配的原则－－学习“十六大”报告有感

十六大在收入分配方面第一次提出各生产要素按贡献参与分配的原则.只有用市场化的手段才能衡量出各生产要素的贡献大小,使按贡献分配原则得到确立.同时,按贡献分配原则也要与按劳分配为主,多种分配方式并存的基本分配制度有机结合.     

马克思主义理论创新的重要文献——全面深入地把握十六大报告的主要精神

党的十六大报告是一篇重要的马克思主义纲领性文献,其理论意义和实践意义是多方面的。十六大报告将“三个代表”重要思想确定为新时期党的指导思想,确立了全面建设小康社会的奋斗目标,制定了建设中国特色社会主义的重大举措,开拓了当代中国马克思主义的新境界。