法医学二代测序STR分型准确度与测序深度的关联性评估

目的利用实验数据对法医学二代测序STR分型测序深度与分型结果准确度的关联性进行评估。方法使用商业化基因组DNA制备单一来源和混合的DNA样本,以Thermo Fisher公司的25重早期测试试剂盒进行目的STR片段扩增,每种扩增产物分别使用4种不同的序列标签平行建库,并控制标记每一种序列标签的文库上机量依次占一张Ion 318芯片的1/4、1/8、1/16、1/32。经Ion PGMTM基因测序仪测序,以及Ion Torrent SuiteTM软件进行数据分析;同时对庞敬博等人发表的基于相同试剂盒和测序仪检测的95名中国汉族无关个体的6928条等位基因、影子峰和噪音序列进行测序深度统计分析,寻找测序深度与STR分型准确度的关联性。结果各基因座测序深度随文库上样量减少而呈明显下降趋势。对于单一来源样本,每张芯片上样不超过8个均一化文库可实现全部基因座的完整分型;对于1∶20比例的混合DNA,每张芯片上样不超过4个均一化文库时,未发现微量组分的等位基因丢失。人群数据测序深度统计显示,该体系基因座间存在不均衡性,有必要针对各基因座分别设定分析阈值参数。结论测序深度与法医学STR分型结果的准确性密切相关,各基因座最低测序深度与平均测序深度的比值可作为设定分析阈值的重要参考指标。本研究确定的单张芯片上样数量仅适用于本实验体系,但相关实验设计和方案可供其他实验体系开展类似工作参考。     

Confidence in an election authority and satisfaction with democracy: Evidence from a quasi-natural experiment of a failed election in Sweden

Contemporary research on electoral integrity has focused on the functioning, evaluation, and legitimacy of electoral processes in emerging democracies. By contrast, this study investigates whether a failed election in a well-established democracy can affect individuals' evaluations of the electoral management body, the Election Authority, and whether those evaluations affect satisfaction with democracy. Using the case of a Swedish regional election in 2010 that had to be rerun due to procedural mistakes in the vote handling, we found that, in the short term, individuals’ confidence in the Election Authority was reduced after it was announced that the election had to be rerun because of the mistakes. Subsequently, this decreased confidence was strongly associated with less satisfaction with democracy at the regional and national level. As good news for the authority, after a successful rerun election, confidence rebounded to the levels prior to the failed election.     

Evaluating the cross-national comparability of survey measures of political interest using anchoring vignettes

Making cross-groups comparisons by using survey instruments has raised substantial scholarly concerns due to the potential risk of incomparability resulting from differential item functioning (DIF). However, not every survey item necessarily suffers from DIF. In this paper, we argue that, unlike many other survey items (e.g., political efficacy), the usual question used to measure political interest is likely to be largely immune to DIF. Our theoretical argument centers on the relative specificity of the item and a corresponding cultural homogeneity (at least in advanced democracies) in what it means to be politically interested or not. Utilizing the anchoring vignettes technique (King et al., 2004; King and Wand, 2007) in our original surveys in the UK, France, and the Netherlands, we demonstrate the size of DIF is small for the standard political interest question.     

基于X-射线衍射法检测甲基苯丙胺晶体并推断来源

目的采用X-射线衍射法(XRD)建立一种快速检测甲基苯丙胺晶体并推断其来源的方法。方法对18起案件中的53份甲基苯丙胺样本进行XRD检验。结果通过分析主要特征峰强度比例,可以得到毒品的定性分析结果,以及样本的晶型取向信息;通过分析XRD谱图中毒品的特征峰半峰宽信息可以得到毒品的结晶度;对样本中明显的杂质峰进行比对,即可得到毒品来源信息参考。结论该方法简便快捷、无损样本、无污染,能够快速、准确、高效地分析结晶状毒品,为确定制贩毒集团上下线及涉毒案件串并案提供佐证。     

对下调法定最低刑责年龄的省思

此次刑法修正案(十一)对最低刑事责任年龄进行下调,可以在很大程度上避免一部分已满12周岁未满14周岁的未成年人倚仗法律保护肆意实施危害行为的"钻法律之空子"的情况发生,能够有效提升法律的威慑力,使公众免受"12岁恶魔"的伤害。在作为调整对象的低龄未成年人一侧,最低刑责年龄的下调意味着他们必须为自己的不法行为承担责任、付出代价。戴上这只法律"金箍"的未成年人将不得不在法律的框架内保持自身行为的检点合法。当然,虽然法律对最低刑事责任年龄进行了下调,但犯罪未成年人仍然需要在"教育为主、惩罚为辅"的矫治环境中得到救赎,既不能"一放了之",也不能"一关了之"[1]。在"疏"与"堵"并行的同时加强相关法律制度的衔接,使低龄未成年人可以在一个相对完善的制度设计中树立起对法律以及他人权益的敬畏之心。     

Chick lit politics in a post-truth era: tricksters,blessees and postfeminist girlpower in Angela Makholwa’s The Blessed Girl

The possibility of an emancipated politics in chick lit novels remains a contested question among postfeminist critics. Drawing on definitions of postfeminism as a transnational sensibility, this article examines South African chick lit in relation to what has been termed post-truth or trickster politics in the context of the rise of politicians, such as Donald Trump. I read Angela Makholwa’s novel The Blessed Girl as an example of African chick lit that features a blessee narrator, a young woman who lives a luxurious lifestyle financed by older men, who is deeply influenced by a Trumpian mode of self-making. By employing a trickster aesthetic and narrative strategies, such as unreliable narration and reader address, the novel, I argue, however also unsettles established parameters of neoliberal girlpower, moving beyond its assumed anti-politicalness, as well as a simple understanding of the blessee figure through either a lens of victimhood or amorality.     

2,4,6-Tri(2′-pyridyl)-1,3,5-triazine for determination of iron(II), Iron(III), and total iron contents on human palms

When iron-based tools, such as knives or guns, are held, traces of iron can transfer to the skin. However, no previous studies have been published regarding the effect of the elapsed contact time on the transfer of iron species with different valences to the palm. Compared with 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine (PDT), 2,4,6-tri(2′-pyridyl)-1,3,5-triazine (TPTZ) was found to exhibit a higher sensitivity to iron(II) spectrophotometrically. This work employed 2,4,6-tri(2′-pyridyl)-1,3,5-triazine (TPTZ) and UV spectrophotometry to determine the amounts of iron(II), iron(III), and total iron transferred to human palms from iron tools. It was found that the palmar moisture level was an important factor in determining the amounts of total iron, including iron(II), transferred to the palm. For identical contact times, the amounts of total iron transferred to the palm was proportional to the palmar moisture, and the difference between the maximum and minimum amounts was 12 μg per hand. However, the amounts of iron(II) transferred to the palm gradually decreased over time for low palmar moisture levels, but steadily increased over time for high palmar moisture levels. Additionally, for average levels of palm moisture, the amounts of iron(II) and iron(III) transferred to the palm gradually decreased and increased, respectively, with longer contact times. Notably, this research could serve as a theoretical basis and guide for the detection of trace iron species with different valences on human palms for criminal investigations.     

Characterization of an odor permeable membrane device for the storage of explosives and use as canine training aids

The storage and use of explosives is regulated at the state and federal level, with a particular focus on physical security and rigorous accounting of the explosive inventory. For those working with explosives for the training and testing of explosive-detecting canines, cross-contamination is an important concern. Hence, explosives intended for use with canine teams must be placed into secondary storage containers that are new, clean, and airtight. A variety of containers meet these requirements and include screw-top glass jars (e.g., mason jars). However, an additional need from the explosive-detecting canine community is secondary containers that can also be used as training aids whereby the volatiles emitted by explosives are emitted in a predictable and stable manner. Currently, a generally accepted method for the storage of explosives and controlled emission of explosive vapor for canine detection does not exist. Ideally, such containers should allow odor to escape from the training aid but block external contaminates such as particulates or other volatiles. One method in use places the explosive inside a permeable cotton bag when in use for training and then stores the cotton bag inside an impermeable nylon bag for long-term storage. This paper describes the testing of an odor permeable membrane device (OPMD) as a new way to store and deploy training aids. We measured the evaporation rate and flux of various liquid explosives and volatile compounds that have been identified in the headspace of actual explosives. OPMDs were used in addition to traditional storage containers to monitor the contamination and degradation of 14 explosives used as canine training aids. Explosives were stored individually using traditional storage bags or inside an OPMD at two locations, one of which actively used the training aids. Samples from each storage type at both locations were collected at 0, 3, 6, and 9 months and analyzed using Fourier Transform Infrared (FTIR) Spectroscopy and Gas Chromatography–Mass Spectrometry (GC–MS) with Solid-Phase Microextraction (SPME). FTIR analyses showed no signs of degradation. GC–MS identified cross-contamination from ethylene glycol dinitrate (EGDN) and/or 2,3-dimethyl-2,3-dinitrobutane (DMNB) across almost all samples regardless of storage condition. The contamination was found to be higher among training aids that were stored in traditional ways and that were in active use by canine teams.     

国家治理现代化与执政方式的完善

执政者是国家治理中最为关键的能动者,其执政方式的合理与否直接决定着国家治理的效果,也关系到执政者的地位。中国共产党是国家治理现代化的倡导者和推动者,也因应着现代化的需要不断进行着自身的现代化。党的执政方式有很强的历史传承性,深受党的革命历程和成功经验的影响。党建立新中国后,开始在自己创建的国家治理体系中进行执政方式的现代化探索,当下正进行第三次现代化的探索,要根据国家治理体系和治理能力现代化的总要求抓住关键环节,加以改进和提高。