The regular article tracking developments at the national level in key European countries in the area of IT and communications – Co-ordinated by Herbert Smith LLP and contributed to by firms across Europe

This column provides a concise alerting service of important national developments in key European countries. Part of its purpose is to compliment the Journal's feature articles and Briefing Notes by keeping readers abreast of what is currently happening “on the ground” at a national level in implementing EU level legislation and international conventions and treaties. Where an item of European National News is of particular significance, CLSR may also cover it in more detail in the current or a subsequent edition.     

用ELISA检测精液及精斑中精子抗原的研究

本实验应用单克隆抗人精子抗体和酶标记羊抗人精子抗体,采用ELISA方法确定精子抗原成份的存在。对10份新鲜精液,15份精斑进行了验测,其结果阳性率为100%。新鲜精液(精子数约10,000万个/ml)稀释100万倍,精斑浸出液稀释50万倍,均可出现阳性。对唾液斑、尿斑、乳汁斑、阴道斑、汗斑及输精管结扎的精液均为阴性。实验结果表明,本法检验精子抗原具有灵敏度高,特异性好的优点。     

The Impact of Quality Public Service Delivery on Customer Satisfaction in Bahir Dar City Administration: The Case of Ginbot 20 Sub-city

ABSTRACT

This study is conducted with the general objective of assessing the impact of quality public service delivery on customer satisfaction in Ginbot20 sub-city. To conduct the study correlational research design, and quantitative research approach have been employed. A total of 88 samples both from customers (68) and service providers (20) were selected using simple random sampling and systematic random sampling techniques respectively. Data were collected using questionnaires. Based on the data collected, the study comes up with certain findings. Among the major findings, the perception of customers and service providers on quality service delivery and customer satisfaction is different, responsiveness, tangibility, assurance, and reliability have positively associated with customer satisfaction. However, empathy has negatively associated with customer satisfaction. Generally, the likelihood of customers gets satisfied when there is quality service delivery is 0.84 (84%) while get satisfied with the probability value of 0.038948(3.8948%) in the absence of quality service delivery.     

不同保存时间和不同精子数量精斑样本DNA分型比较

目的比较不同保存时间和不同精子数量精斑样本DNA分型的效果。方法制备精斑样本,保存10d的样本采用激光显微捕获30、20、15、10、5、1个精子,用于不同数量精子分型比较;保存10d、214d、375d的样本分别捕获30、20、10个精子,用于不同保存时间分型比较。比较各组检出率、等位基因丢失率和非特异性扩增率,采用χ2检验进行差异比较。结果①不同精子数量分型：捕获10个精子即可得到完整的DNA分型,且随着精子数增多,检出率逐渐提高而等位基因丢失率逐渐降低,30个精子等位基因丢失率为0%,1个精子则可达58.89%;②不同保存时间分型：总趋势是保存时间越短,捕获精子越多检出率越高,10个精子与20、30个精子组比较,均有显著性差异（P〈0.05）;等位基因丢失率及非特异性扩增率则随保存时间的延长而增加,相同保存时间的不同精子数量组之间和相同的精子数量的不同保存时间组之间比较,差异均具有统计学意义（P〈0.05）。结论激光显微捕获精子数目和检材保存时间对DNA分型结果有直接影响。     

浅析网络平台服务商的名誉权侵权责任

网络的匿名特点使人们可以自由地发表言论而不会轻易被发现,民事主体的名誉权在网络环境下显得异常脆弱,越来越多的网络服务提供商卷入了侵权诉讼中。其中网络平台服务商应否对第三人的诽谤行为承担责任成为网络环境下亟待解决的问题。综合考量世界各国的立法和实践,结合我国的实际,在界定网络平台服务商责任时应以最低程度的注意义务和过错责任为宜。     

Microchip-based cell lysis and DNA extraction from sperm cells for application to forensic analysis

The current backlog of casework is among the most significant challenges facing crime laboratories at this time. While the development of next-generation microchip-based technology for expedited forensic casework analysis offers one solution to this problem, this will require the adaptation of manual, large-volume, benchtop chemistry to small volume microfluidic devices. Analysis of evidentiary materials from rape kits where semen or sperm cells are commonly found represents a unique set of challenges for on-chip cell lysis and DNA extraction that must be addressed for successful application. The work presented here details the development of a microdevice capable of DNA extraction directly from sperm cells for application to the analysis of sexual assault evidence. A variety of chemical lysing agents are assessed for inclusion in the extraction protocol and a method for DNA purification from sperm cells is described. Suitability of the extracted DNA for short tandem repeat (STR) analysis is assessed and genetic profiles shown. Finally, on-chip cell lysis methods are evaluated, with results from fluorescence visualization of cell rupture and DNA extraction from an integrated cell lysis and purification with subsequent STR amplification presented. A method for on-chip cell lysis and DNA purification is described, with considerations toward inclusion in an integrated microdevice capable of both differential cell sorting and DNA extraction. The results of this work demonstrate the feasibility of incorporating microchip-based cell lysis and DNA extraction into forensic casework analysis.     

论网络侵权责任中的反通知及效果

随着互联网科技的不断发展,人们日常生活对网络的依赖有增无减。人们在享受网络带给生活的巨大便利的同时,也倍受各种网络侵权行为的干扰。《侵权责任法》第36条为解决网络侵权问题找到了出口,但除了该条第2款中明确规定的被侵权人享有向网络服务提供者发出通知的权利之外,侵权网络用户也享有向网络服务提供者发出反通知的权利。这样才能平衡网络侵权中以网络服务提供者为中心的被侵权人、侵权网络用户和其他网络用户之间的利益。     

“互联网条款”对于新类型网络服务的适用问题——从“通知删除”到“通知加采取必要措施”

"通知删除"规则率先确立于《信息网络传播权保护条例》,《侵权责任法》"互联网条款"将其扩展为"通知加采取必要措施",并使其广泛适用于一般网络侵权领域。《电子商务法》知识产权保护条款又有相应的规定。随着互联网技术和商业模式迅速发展,新类型网络服务层出不穷,在适用"通知删除"等规则中不断产生新问题和新争议。除另有特别规定外,新类型网络服务应当适用《侵权责任法》第36条规定的"通知加采取必要措施",其中"必要措施"的界定具有开放性和灵活性,"必要措施"的妥当性取决于与特定网络服务的匹配性,可以根据网络服务提供者的具体情形,以利益衡量的方式确定相应的具体措施。个案中应当根据新类型网络服务的特性以及产业发展需求等情况,合理权衡特定网络服务与"必要措施"的适应性,妥善确定应当采取的"必要措施"。     

激光捕获显微切割技术用于分离混合斑中精子细胞

目的 评估激光捕获显微切割(laser capture microdissection.LCM)技术在分离混合斑中少量精子细胞的应用价值.方法 配制不同比例的精液-阴道上皮细胞混合液.分别用差异裂解法和LCM法分离精子细胞,用磁珠法提取精子细胞DNA,并用IdentifilerTM试剂盒进行STR基因型检测.结果 LC...     

网络服务提供者的侵权责任——以《侵权责任法》第36条第2款、第3款为中心

《侵权责任法》第36条第2款、第3款对网络用户直接侵权时,网络服务提供者承担连带责任的情形进行了规范。但这两款内容过于原则,需要运用解释论的方法予以细化。第36条关于网络服务提供者侵权责任的规定要在权利人、社会公众、网络服务提供者之间达到更为合理的平衡,有待将来的司法解释进行完善。