共查询到19条相似文献,搜索用时 250 毫秒
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目的采用反相高效液相色谱法(RP-HPLC)检测大鼠肌肉组织中7种生物胺,并探讨其在法医学中的应用价值。方法以丹磺酰氯为衍生剂,通过ZORBAX Rx-C18反相色谱柱分离,以乙腈-水作流动相梯度洗脱,流速为1.0mL/min,柱温为20℃,DAD检测器,对色胺、腐胺、尸胺、组胺、酪胺、亚精胺、精胺7种生物胺进行检测。结果 7种生物胺得到良好分离,在0.5~100.0μg/mL的范围内线性良好,相关系数均>0.99,检出限在0.14~0.50μg/mL之间;对大鼠肌肉样本的添加回收率在83.8%~100.6%之间,相对标准偏差均<11%。结论采用RP-HPLC法检测生物胺,方法简便、快速、灵敏,能够满足尸体组织中生物胺检测的需要。 相似文献
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目的建立了生物样品中丁丙诺啡的高效液相色谱-电喷雾串联质谱检测方法。方法样品经固相萃取提取净化、反相液相色谱分离后进行质谱检测,根据保留时间及特征离子进行定性分析,以母离子m/z468进行定量分析。结果在10-500ng/ml(ng/g)范围内峰面积与质量浓度的线性关系良好(r^2〉0.993)。在50、100、500ng/ml(ng/g)3个添加水平,尿、血、肝中丁丙诺啡的平均回收率为74%~94%,日内测定结果的相对标准偏差小于8%,日间测定结果的相对标准偏差小于10%。结论该方法简单、灵敏,特异性强,适用于生物样品中丁丙诺啡的分析检验。 相似文献
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高效液相色谱(HPLC)是一种对大部分有机化合物既能进行分离制备,又能进行检测的现代分析仪器,但由于存在某些原因,该仪器目前在我国应用还不广泛,在刑事科技上的应用那就显得更少了,利用高效液相色谱对杀虫剂的检测,目前尚缺系统的报导,我们根据刑事毒物检验要求快而准的特点.试用单根色谱反相柱,以甲醇/水为流 相似文献
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目的建立卡西酮的高效液相色谱检测方法。方法采用UPLC-DAD分析方法。分析柱:Agilent ZorbaxSB-Phenyl柱(250mm×4.6mm,5μm),流动相为三氟乙酸(pH 3.5)∶乙腈为85∶15,流速0.2mL/min,检测波长254nm。结果卡西酮在0.5~1 000μg/mL浓度范围内线性关系良好R2=0.999 4,日内与日间保留时间和峰面积的标准偏差(RSD)均<1.06%,检出限为0.068μg/mL,平均回收率95.9%。结论本方法峰形好,分离度好,线性范围良好,回收率高,适用于刑事案件中卡西酮的定性定量分析。 相似文献
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作者采用反相高效液相色谱法对氯丙嗪、三氟拉嗪、异丙嗪、奋乃静、氟奋乃静、氯氮平、氟哌啶醇、安泰乐,多虑平等九种常见抗精神失常药物进行分离。并对色谱条件流动相中甲醇比例,二乙胺浓度,pH值及柱温对药物保留时间的影响进行考察,选择出一种能良好分离九种药物的色谱系统,对这类药物中毒时鉴别药物品种具有实用意义。 相似文献
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目的建立苯二氮卓类新精神活性物质去氯依替唑仑的气相色谱-质谱(GC-MS)和超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF MS)定性检验方法。方法未知样品用甲醇和水提取,取上清液,采用GC-MS和UPLC-Q-TOF MS进行分析。结果经GC-MS检测,保留时间为17.73 min的未知组分的质谱碎片主要特征离子峰有m/z 279,308,239,252,225,77,126。经UPLC-Q-TOF MS检测,保留时间为4.781 min的未知组分的准分子离子峰为309.1173,碰撞诱导解离(CID)模式下二级质谱主要离子有m/z 280.0776,255.0952,240.0719,225.0604,206.0748。经缴获毒品分析科学工作组(Scientific Working Group for the Analysis of Seized Drugs,SWGDRUG)分析谱库检索和文献查询获得的信息资料进行比对,鉴定为去氯依替唑仑。结论该方法具有分析简便、快速的特点,可以用于实际案件的检测。 相似文献
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反相液相色谱法检测生物试样中的氧化乐果 总被引:1,自引:0,他引:1
本文报告了利用ODS C_(18)反相柱,乙腈:5mM KH_2PO_4为流动相,检测人体组织中的氧化乐果。组分与内源性杂质分离较好,可以定性分析体内氧化乐果农药。本文方法操作简便、快速,可在实际办案中应用。 相似文献
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同时裂解甲基化气相色谱法快速鉴别人体脂肪及动植物油脂 总被引:4,自引:0,他引:4
采用同时裂解甲基化气相色谱法(SPM-GC),首次分析人体脂肪,并且观察人体脂肪、猪、鸡、牛、羊脂和豆油中7种脂肪酸的组份含量变化。根据7种主要脂肪酸(肉豆蔻酸、棕榈酸、棕榈油酸、硬脂酸、油酸、亚油酸和亚麻酸)甲酯的百分含量可有效地进行鉴别。结果表明:通过SPM-GC法分析油脂,可将传统的油脂酯化法时间由2个多小时缩短到1分钟左右。数据C.V.%<4%,最小检测量为1.Oμg。最佳比例四甲基氢氧化按(TMAH)甲醇液(TMAH:甲醇=1:10,V/V),可消除油脂中多不饱和脂肪酸的异构化和降解。 相似文献
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Adipocere formation is well known as a later post-mortem change. We experienced a female victim who had been sealed up in a clothes box for approximately 4 years. We collected several subcutaneous fats as well as visceral fats from the victim to investigate adipocere formation. Fresh subcutaneous fats of one female and five male victims who suddenly died were used as the control. These samples were homogenized and the lipids were extracted with chloroform and methanol followed by injection into gas chromatography-mass spectrometry and gas chromatography. We detected a hydroxy fatty acid in the fat of the case, but not in the controls. Using standard synthetic hydroxy fatty acid, the lipid extract component was identified as 10-hydroxyoctadecanoic acid (10-OH 18:0) and this concentration was quantified. Consequently we confirmed that adipocere was formed much slowly in dry concealment. In addition, the fatty acid composition was compared with the control. Most of the linoleic acid (18:2) disappeared and a peak developed instead. Using standard synthetic fatty acid, this peak was identified as cis-12-octadecenoic acid (cis-12-18:1). This suggests that linoleic acid is hydrogenated to cis-12-octadecenoic acid in the process of adipocere formation. 相似文献
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Alternative fuels are becoming more prominent on the market today and, soon, fire debris analysts will start seeing them in liquid samples or in fire debris samples. Biodiesel fuel is one of the most common alternative fuels and is now readily available in many parts of the United States and around the world. This article introduces biodiesel to fire debris analysts. Biodiesel fuel is manufactured from vegetable oils and/or animal oils/fats. It is composed of fatty acid methyl esters (FAMEs) and is sold pure or as a blend with diesel fuel. When present in fire debris samples, it is recommended to extract the debris using passive headspace concentration on activated charcoal, possibly followed by a solvent extraction. The gas chromatographic analysis of the extract is first carried out with the same program as for regular ignitable liquid residues, and second with a program adapted to the analysis of FAMEs. 相似文献
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《Forensic science international》1996,79(3):215-226
In a new attempt at species identification, the total composition and positional distribution of fatty acid in triacylglyceride (TG) of adipose and bone tissues were analyzed in human, bovine, pig, dog, cat and chicken tissues. Although the total fatty acid compositions of bovine and pig tissues were significantly different from those of human (different in more than half the fatty acids tested), dog, cat and chicken tissues showed a comparatively similar composition to human fatty acids composed of TG in both tissues. The TG in these tissues was also subjected to stereospecific analysis using pancreatic lipase, that is, the fatty acid distribution in positions 1,3 (not distinguished between 1 and 3) and 2 of the TG were determined. The distribution of fatty acids among the positions 1,3 and 2 in the TG of animal adipose and bone tissues was non-random. The distribution between position 2 and positions 1,3 seems to be governed by chain length and unsaturation in each animal. The shorter and more unsaturated fatty acids showed a greater tendency to occupy position 2 of TG. Although this rule appeared in all animals except the pig, the distribution of each fatty acid into position 2 was species-specific. The positional distribution of fatty acid in TG was identical among the same species and in different regions of the same body. Thus, even when species identification is difficult using the pattern of total fatty acid composition, the analysis of the positional distribution of fatty acid makes it possible to determine the species. From the present results, the evaluation of positional distribution of fatty acid in the TG is a useful tool for the identification of human tissues. 相似文献
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The chemical composition of subcutaneous fats was analyzed in a corpse that had died from drowning. The skin of the cadaver examined postmortem showed different stages of adipocere. Samples from these regions were chemically compared with the fatty tissue of a person who had died recently. HPLC, GC, GC-MS, NMR (1H- and 13C-NMR), TLC and titrimetrical methods were used to evaluate the degree of decomposition. The fatty acid pattern of the triglycerides (TG) and the free fatty acids (FFA) obtained by TLC separation was also investigated. Some discrepancy was observed between the autopsy findings and the results of the chemical analysis. It is suggested that the autopsy should be supplemented by chemical analysis in order to describe the state of adipocere correctly. 相似文献
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线粒体16srRNA和ND4基因在种属鉴定中的应用研究 总被引:2,自引:1,他引:1
目的构建一种用于种属鉴定的线粒体DNA(m tDNA)16 srRNA和ND4基因荧光标记复合扩增检测体系。方法利用引物设计软件(Prim er 5)对两个m tDNA序列ND4基因和16 srRNA基因设计两对引物,每对引物中的一条在5’端标记荧光素(6-FAM)。按传统复合扩增技术建立复合扩增体系,用AB I PR ISM 310基因分析仪对产物进行分析。结果人类DNA扩增产物出现两个峰,片段大小分别为110bp的人类特异片段和149bp的人与动物共有片段,而动物DNA扩增产物出现一个峰,片段大小为149bp。对30个实验室存放5~15年的陈旧人血痕也能明确判断其种属来源。结论该体系可以明确区分人源性生物检材与其它常见动物样本,对实验室长期存放的陈旧检材也具有较好的检测能力。 相似文献
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Gas-chromatography-mass spectrometry was used to characterise the fatty acids from soils and associated tissues excavated from a 1967 Foot and Mouth burial pit. Subcutaneous fats were mainly comprised of 55-75% palmitic acid, 17-22% stearic acid and 3-16% oleic acid as well as 5-7% myristic acid. The distribution of fatty acids confirmed that the tissues were decayed to adipocere. The loss of oleic acid to <3% in two of the decayed fats suggested advanced stages of adipocere. However, adipocere formation was limited in a third tissue sample recovered from greater depth. Inductively coupled plasma atomic emission spectrometry of the pore waters revealed a decrease in Ca concentration and concurrent increase in Na concentrations this suggested that insoluble calcium salt had formed through displacement of sodium. The use of fatty acid profiles from soils and soil interstitial pore waters provide complementary evidence of adipocere formation in foot and mouth burial pits. 相似文献
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Food labeling: trans fatty acids in nutrition labeling,nutrient content claims,and health claims. Final rule 总被引:2,自引:0,他引:2
The Food and Drug Administration (FDA) is amending its regulations on nutrition labeling to require that trans fatty acids be declared in the nutrition label of conventional foods and dietary supplements on a separate line immediately under the line for the declaration of saturated fatty acids. This action responds, in part, to a citizen petition from the Center for Science in the Public Interest (CSPI). This rule is intended to provide information to assist consumers in maintaining healthy dietary practices. Those sections of the proposed rule pertaining to the definition of nutrient content claims for the "free" level of trans fatty acids and to limits on the amounts of trans fatty acids wherever saturated fatty acid limits are placed on nutrient content claims, health claims, and disclosure and disqualifying levels are being withdrawn. Further, the agency is withdrawing the proposed requirement to include a footnote stating: "Intake of trans fat should be as low as possible." Issues related to the possible use of a footnote statement in conjunction with the trans fat label declaration or in the context of certain nutrient content and health claims that contain messages about cholesterol-raising fats in the diet are now the subject of an advance notice of proposed rulemaking (ANPRM) which is published elsewhere in this issue of the Federal Register. 相似文献