老板书架

书名:《胡润百富榜:中国富豪这十年》作者:(英)胡润(中)东方愚出版社:中信出版社定价:39元内容简介:1999年,英国青年胡润在上海滩左冲右突,闯不出名堂。胡爸爸的一句话点醒梦中人,胡润开始了自己头号榜主的生涯。百富榜是他的东风。荣毅仁、黄光裕、张茵、牟其中、王石、黄宏生、李宁、唐万新……一个个富豪你方唱罢我登场,喧嚣无比。胡润也因此成为中国炙手可热的人物。     

附件:《民事程序法研究》注释规范

1.一律采用页下注,每页重新编号。全书统一。2.引用他人著作应依次标明作者、书名、出版社、出版时间、页码。第一版的著作无须注明版次。例如:①唐长孺:《魏晋南北朝史论丛》,三联书店1955年版,第158～161页。②齐树洁主编:《民事司法改革研究》,厦门大学出版社2006年第3版,第2页。3.引用他人论文,应依次标明作者、论文题目、报刊名称、刊期(或日期),但不必注明页码。例如:①周一良:《关于崔浩国史之狱》,载《法学研究》1982年第4期。②谢重光:《略论唐代寺院、僧尼免赋特权的逐步丧失》,载何兹全主编:《五十年来汉唐佛教寺院经济研究》,北京师范大学出版社1986年版。4.引用译作,应依次标明国籍、原作者、著作名称、译者、出版社、出版时间、页码。例如:[美]罗伯特·C.埃里克森:《无需法律的秩序——邻人如何解决纠纷》,苏力译,中国政法大学出版社2003年版,第154页。5.转引他人著作的注释方式,例如:     

微博

@王石V:采访问:那么忙,哪有空读书?答:读书成为一种习惯,就像吃饭、睡觉,你就会有时间。问:正在读什么?答:有关日本江户时代史,有20本吧,像贝拉的《德川宗教》、艾森斯塔特的《日本文明》、汉德森的《德川时代法律的演变》……问:推荐一本书的话?答:最近几年推荐盐野七生的《罗马人的故事》,一套15本。     

幼儿误服煤油中毒死亡1例

1案例案情:某幼儿,16个月。因误服煤油约20mL后咳嗽、口唇青紫、口吐白沫、昏迷1小时于当日9:15入院救治,先门诊洗胃,后入住儿科,予以告病危、输氧、抗感染、对症、输液、利尿等处理。10:30插胃管准备用石蜡油中和煤油和导泻,因不配合未用药立即拔管,11:00出现呼衰,予高频呼吸机、呼吸兴奋剂、肾上腺素及心肺复苏抢救无效于11:30死亡。尸检:男性幼儿冷冻尸体一具,尸长85cm,重10.5kg发育正常,营养一般。尸斑暗紫红色。球结膜苍白,睑结膜充血。口唇轻度紫绀、口腔粘膜充血。指甲青紫,趾甲苍白。额部见散在皮下出血点。脑膜血管扩张充血,脑沟…     

欢迎订阅2014年《中国法医学杂志》

《中国法医学杂志》是由中华人民共和国公安部主管、中国法医学会主办、《中国法医学杂志》社出版的综合性学术期刊。大16开,90页,双月末出版,国内外公开发行。每期定价24元,全年6期,共计144元。订购办法:·邮局汇款:北京市西城区木樨地南里17号楼收款人:《中国法医学杂志》社,邮政编码:100038·银行汇款:中国工商银行北京白云路支行账号:0200020009200922863户名:《中国法医学杂志》社     

国家检察官学院 朱丽欣教授简介

正朱丽欣,女,国家检察官学院教授,刑事检察教研部主任。毕业于吉林大学法学院,法学硕士。学术兼职:中国法学会会员、中国犯罪学研究会常务理事、北京市预防跨国犯罪研究会理事。研究方向:刑法学、犯罪学讲授课程:刑法学、检察制度与实务、法律职业伦理     

诉讼时效不因履行而重新确认

【裁判要旨】诉讼时效的中断,只能发生在诉讼时效期间内,超过了诉讼时效期间的履行行为则不引起诉讼时效的中断,也不能就此推定为诉讼时效的重新确认。口案号一审:(2013)莱城民重初字第5号二审:(2013)莱中民四终字第81号【案情】原告:杨国明。被告:陈新强,莱芜市博庚物资有限公司总经理。被告:李雪梅、谷国利、陈永俊、白振娟。被告:莱芜市博庚物资有限公司。原告杨国明诉称:2009年11月7     

双大腿创伤性骨化性肌炎1例

1案例李某,男,17岁。2003年6月被人用木棍夹伤双上肢,双下肢被人反复踢伤,伤后次日到当地医院就诊。主诉:“右上肢手无力、双下肢下蹲受限2天。”查体:神志清楚,对答切题。四肢可见大片皮下出血,双下肢肿胀明显。X线:未见骨折征象。诊断:“四肢软组织损伤。”伤后5个月,因“双下肢不能屈曲、右手肌肉萎缩”再次就诊。临床查体:右手大鱼际肌萎缩,外展、对掌力弱,右手拇指、食指、中指、无名指桡侧感觉减退。双下肢近端肌力Ⅳ级,远端Ⅴ级,四肢腱反射对称存在,双侧病理征阴性。血液检验报告:碱性磷酸酶:266.2U/L,肌酸激酶正常。颈段MRI:未见…     

书讯

《每个人都是改革的缔造者:蔡定剑论民主、法治与人权》作者:蔡定剑编者:刘小楠许婕定价:35元出版:2011年11月本书以"民主、法治、人权"为主题,精心挑选了60篇文章。正如张千帆所言:民主是立国之本,是决定每个人命运的根本制度;没有民主,法治也只能是空谈;     

混合生物样品的组分分析及其STR基因型判定

目的应用荧光标记STR多基因座联合检测技术对混合生物样品较少组分最低比例检出限和STR基因型的判定进行研究。方法 将已知浓度的人标准细胞系DNA:9947A和K562分别按照1:1、1:4、1:9、1:19、1:39、1:59、1:79和1:99比例混合后作为扩增模板,应用Profiler plus试剂盒对D3S1358、VWA、FGA、D8S1179、D21S11、D18S51、D5S818、D13S317、D7S820和性别鉴定基因座检验。结果在比例为1:19时能明确判定两个体各基因座基因型,且两样品基因型结果峰高平均值之比与样品浓度之比正相关。结论应用荧光标记STR多基因座联合检测技术可以对一定比例的混合生物样品进行STR基因型判定,并对其混合比例状况进行大致推断。     

液相色谱-串联质谱法检测血液中的美西律

目的建立测定血液中美西律(mexiletine)的液相色谱-串联质谱联用法(LC-MS/MS)。方法采用简便的乙腈蛋白沉淀法对血液进行预处理,应用Allure PFP Propyl液相柱分离,用电喷雾正离子模式离子化,多反应监测模式对美西律进行分析。结果美西律与内标纳洛酮分离良好,在0.02～10.00μg/mL内线性关系良好,相关系数为0.9999,回归方程为y=0.0283x-0.0151,日内与日间精密度的RSD均小于15%,最低检测限为0.01μg/mL。结论建立的LC-MS/MS方法简单、灵敏、可靠,可同时适用于美西律临床药物监测和法医毒物分析的需要。     

血清中盐酸曲马多的膜式固相萃取及GC／MS／SIM测定

目的建立血清中盐酸曲马多的膜式固相萃取(SPE)气-质联用分析方法。方法1mL血清用2mL0.1mol/L磷酸盐缓冲液(pH6)稀释后,用含有C18和强酸型强阳离子交换基团的SPECC18AR/MP3固相萃取柱萃取,洗脱液为含2%氨水的乙酸乙酯;选择SKF525作为内标物,用GC/MS/SIM定量测定。结果在加标量为0.1、0.2和0.5μg/mL的血清样品中,盐酸曲马多的回收率分别为98.9%、92.5%和84.8%,5次测定的RSD分别为3.2%、8.7%和10.9%;线性范围为0.1～4μg/mL,多项式回归相关系数r2=0.9939;检出限为21ng/mL;同一根萃取柱,连续使用5次,没有出现堵塞和污染,回收率及RSD未见下降。结论本方法适合于法医毒物分析。     

血液中丙泊酚的GC-MS分析

目的应用气质联用仪(GC-MS),建立快速、灵敏地分析血液中丙泊酚的定性定量方法。方法血液1mL,加入内标,采用液液提取方法处理,GC-MS选择离子(SIM)模式测定。结果在0.1～10μg/mL范围呈良好的线性关系,相关系数R2为0.9931,最低检测限(LOD)为0.05μg/mL。结论所建立的方法简便、快速、特异性强、灵敏度高,可满足法庭毒物分析和临床毒物分析的需要。     

LC-MS／MS测定尿液中可卡因及其代谢物苯甲酰爱康宁

目的建立尿液中可卡因(cocaine,COC)及其代谢物苯甲酰爱康宁(benzoylecgonine,BZE)的液相色谱-串联质谱分析方法。方法尿液经固相萃取后,用AllurePFP丙基柱分离,以V(甲醇):V(20mmol/L乙酸胺和0.1%甲酸的缓冲溶液)=80∶20为流动相,采用二级质谱多反应监测模式检测COC和BZE。按10mg/kg的剂量对豚鼠腹腔注射可卡因,给药后收集7d尿液。结果尿液中COC和BZE在2.0～100ng/mL质量浓度范围内线性关系良好(r=0.9995),最低检测限(LOD)为0.5ng/mL;回收率大于90%;日内和日间精密度均小于6%;豚鼠尿液中主要检测目标物是BZE,且BZE检测时限也较COC长。结论所建方法灵敏度高,选择性好,适用于尿液中可卡因和苯甲酰爱康宁的检测。     

Simultaneous identification/determination system for phentolamine and sildenafil as adulterants in soft drinks advertising roborant nutrition

An easily available, simultaneous identification/determination procedure for phentolamine (PHE) and sildenafil (SIL) in adulterated dietary supplements was established by using a combination of three different analytical methods; thin-layer chromatography (TLC), liquid chromatography-mass spectrometry (LC/MS) and a high-performance liquid chromatography (HPLC)/photo-diode-array. The sample solution for TLC was applied to silica gel 60 F(254) plates with chloroform/ammonia solution (28)/methanol (70:5:3, lower layer) and chloroform/diethylamine/methanol (15:3:2) as the developing solvent. Spots were located under UV radiation at 254 nm. Mass spectra of PHE and SIL by LC/MS were investigated with electrospray ionization (ESI) interface, under both positive and negative ion mode. The HPLC analysis was performed on a column of Wakosil 5C18 (4.6 mm x 150 mm, 5 microm) with water/methanol/acetonitrile/triethylamine (580:250:170:1) adjusted with phosphoric acid to pH 3.0 as the mobile phase, and the effluent was monitored with a photo-diode-array detector. Quantitative HPLC analysis of PHE and SIL were detected at 280 nm. When this procedure was applied to commercial soft drinks, PHE and SIL were identified and determined at a concentration of 17 mg PHE and 44 mg SIL per bottle, respectively. The procedure described here is available for the screening of PHE and SIL in adulterated supplements.     

Postmortem tissue distribution of olanzapine and citalopram in a drug intoxication

A 40-year-old white male was found dead in bed in a group home for mentally ill adults. The decedent had been diagnosed a paranoid schizophrenic. An autopsy was performed at the Office of the Cuyahoga County Coroner in Cleveland, Ohio. Toxicological testing detected olanzapine and citalopram in post mortem specimens. Multiple fluids and tissues were assayed by liquid-liquid extraction followed by gas chromatography with nitrogen phosphorus detection, and qualitative confirmation by electron impact gas chromatography/mass spectrometry. Drug concentrations [olanzapine: citalopram; mg/L or mg/Kg] determined in this case are the highest reported to date involving these drugs- 1.38:3.35 heart blood, 1.11:1.65 femoral blood, 60.24:32.43 urine, 6.47:10:71 liver, and 38.36:49.16 lung, respectively. Drug concentrations in tissues were found to be the highest in lung for both drugs and lowest in the heart. Citalopram but not olanzapine was detected in bone. The cause of death was ruled acute intoxication by the combined effects of olanzapine and citalopram and the manner, accident.     

Aqueous phase hexylchloroformate derivatization and solid phase microextraction: determination of benzoylecgonine in urine by gas chromatography-quadrupole ion trap mass spectrometry.

A derivatization/solid phase microextraction (SPME) method for the determination of benzoylecgonine in urine was developed. The derivatization is conducted directly in 1 mL of urine while sonicating for 3 min with 12 microL of hexyl chloroformate and 70 microL of a mixture containing acetonitrile:water:hexanol:2-dimethylaminopyridine (5:2:2:1 v/v), yielding benzoylecgonine hexyl ester (BHE) as the product. After the 3 min period, an aliquot of 250 microL is transferred to a vial for SPME. After the desired extraction time the 100 microns polydimethylsiloxane SPME fiber was transferred to the GC-MS for separation and analysis with a quadrupole ion trap mass spectrometer. The hexyl chloroformate derivatization and SPME procedures were optimized for compatibility and sensitivity. The method was found linear for 0.10 to 20.0 micrograms/mL (r2 = 0.999) of benzoylecgonine in urine using benzoylecgonine-d3 as an internal standard (1.5 micrograms/mL). Intra-day precisions were 8.8 and 6.8% RSD for 0.30 microgram/mL and 17 micrograms/mL benzoylecgonine standards in urine (n = 6), respectively. Inter-day precision (n = 3) were < or = 3.3% RSD, indicating good reproducibility. A detection limit of 0.03 microgram/mL (S/N = 3) was achieved, thus making the SPME method a simplified alternative to SPE for GC-MS confirmation after EMIT tests for benzoylecgonine which have a cutoff of 0.30 microgram/mL. Quantitative results by SPME and SPE of two clinical urine specimens known positive for cocaine by EMIT were in excellent agreement. Benzoylecgonine was detected by the derivatization/SPME method in 22 out of 22 other urine specimens known positive for cocaine.     

血液、尿液中氯胺酮及其代谢物去甲氯胺酮的HPLC分析

目的 建立血液、尿液中氯胺酮及其代谢物去甲氯胺酮的高效液相色谱(HPLC)分析方法.方法 以非那西丁为内标,检材加入10%的氢氧化钠溶液调节pH值为14,用甲苯提取,离心后取有机层,水浴下吹干,乙腈定容后进HPLC仪分析.结果 检测血液中氯胺酮和去甲氯胺酮的线性范围均是0.05～10μg/mL(r2＞0.999 3),检测尿液中氯胺酮和去甲氯胺酮的线性范围均是0.01～50 μg/mL(r2＞0.999 5).氯胺酮和去甲氯胺酮在血液和尿液中的检测限分别是0.006 μg/mL和0.003 μg/mL.血液和尿液中氯胺酮和去甲氯胺酮的回收率不低于82.4%.检测血液和尿液中氯胺酮和去甲氯胺酮的日内精密度和日间精密度均小于10.0%.将所建的方法应用于给大鼠氯胺酮后的血液和尿液中的氯胺酮和去甲氯胺酮的测定,得到了氯胺酮和去甲氯胺酮在大鼠的药时曲线和尿排药速率曲线. 结论本方法简便、快捷,适用于血液、尿液中氯胺酮及其代谢物去甲氯胺酮的分析.     

Simultaneous identification/determination system for phentolamine and sildenafil as adulterants in soft drinks advertising roborant nutrition

An easily available, simultaneous identification/determination procedure for phentolamine (PHE) and sildenafil (SIL) in adulterated dietary supplements was established by using a combination of three different analytical methods; thin-layer chromatography (TLC), liquid chromatography–mass spectrometry (LC/MS) and a high-performance liquid chromatography (HPLC)/photo-diode-array. The sample solution for TLC was applied to silica gel 60 F₂₅₄ plates with chloroform/ammonia solution (28)/methanol (70:5:3, lower layer) and chloroform/diethylamine/methanol (15:3:2) as the developing solvent. Spots were located under UV radiation at 254 nm. Mass spectra of PHE and SIL by LC/MS were investigated with electrospray ionization (ESI) interface, under both positive and negative ion mode. The HPLC analysis was performed on a column of Wakosil 5C18 (4.6 mm×150 mm, 5 μm) with water/methanol/acetonitrile/triethylamine (580:250:170:1) adjusted with phosphoric acid to pH 3.0 as the mobile phase, and the effluent was monitored with a photo-diode-array detector. Quantitative HPLC analysis of PHE and SIL were detected at 280 nm. When this procedure was applied to commercial soft drinks, PHE and SIL were identified and determined at a concentration of 17 mg PHE and 44 mg SIL per bottle, respectively. The procedure described here is available for the screening of PHE and SIL in adulterated supplements.     

LC-MS/MS-MRM筛选血液中22种有毒生物碱成分

目的建立液相色谱-串联质谱法对血液中的22种常见有毒生物碱成分进行筛选分析。方法血液以丁丙诺菲为内标经液液提取后,用液相色谱-串联质谱仪以电喷雾电离(ESI )、多反应监测(MRM)方式进行分析。结果以化合物的保留时间、两对母离子/子离子对定性,最低检出限为0.1～20ng/mL。结论该方法选择性佳、灵敏度高,适用于法医毒物分析和临床毒物分析中有毒生物碱的分析。