Integrated DNA and Fingerprint Analyses in the Identification of 60‐Year‐Old Mummified Human Remains Discovered in an Alaskan Glacier

Abstract: This report describes the identification of a merchant mariner who perished in 1948 when Northwest Airlines Flight 4422, a DC‐4 carrying 24 seamen and six crew members crashed into Mount Sanford, Alaska. Fifty‐one years later, a human forearm and hand were found close by the wreckage of the plane, prompting identification efforts using DNA and fingerprints. There were significant challenges to both the fingerprint and DNA analyses. The hand was badly desiccated, making fingerprint friction‐ridge detail almost invisible and the remains had been embalmed upon discovery, making DNA amplification difficult. We present the results of an interdisciplinary approach that successfully addressed these challenges and ultimately led to the identification of the remains. These efforts relied on efficient fingerprint rejuvenation and imaging techniques that improved print resolution, as well as new DNA extraction techniques optimized for aggressively embalmed remains.     

A new method of reproduction of fingerprints from corpses in a bad state of preservation using latex

In view of the problems arising while fingerprinting corpses in a bad state of preservation, especially in case of mummification and carbonization, the authors propose an innovative technique which uses latex film. To better illustrate the potential of the method, two cases where the latex technique was applied successfully are reported: the first one is a mummified body discovered in a shack on the outskirts of Milan and the second one is the case of a burnt corpse found in a car boot. Such a technique is versatile, easy to apply, and allows the operator to work quickly on cadavers without amputating parts, except in rare cases (i.e., burnt bodies with muscle retraction). By the latex technique, a perfect and enduring negative copy of the fingerprint is obtained, ready to be inked and photographed. The numerous copies produced this way can be inked several times allowing for the repeatability of the procedure and this is crucial in cases of problematic legal identification of corpses. In both the cases illustrated, the fingerprints obtained by the latex technique were useful for identification. The quality was good enough for the automatic fingerprint identification system research system to be applied.     

The effect of electrostatic fingerprint visualization on integrated ballistic identification systems

Visualization of fingerprint corrosion on spent brass cartridge cases by the application of a high electrical potential and conducting carbon powder is becoming an accepted method of fingerprint enhancement. However, to date, no examination has been made of any effect this technique has on ballistic identification. To resolve this, images of the breech face and firing pin marks were captured on six plated nickel and six brass primer cup spent cartridge cases. Three nickel and three brass cases were then subjected to the application of a potential of +2500 V for a period of 1 min. The remaining cases were additionally subjected to the application of carbon powder. These latter cases were then washed to remove all traces of powder. Each case was recaptured with the same ballistic identification apparatus and imaging procedure. None of the twelve cases showed any visual difference after the application of the potential or conducting powder.     

Demonstration of a gastric bioptic specimen mix-up by laser capture microdissection (LCM) and DNA fingerprinting

We demonstrate here the successful use of laser capture microdissection (LCM) and DNA fingerprinting in the identification of a case of gastric bioptic specimen mix-up. A 70-year-old man, suffering from chronic atrophic gastritis, underwent to a gastric biopsy and received a diagnosis of gastric cancer. In the absence of any clinical evidence of gastric cancer, a specimen mix-up was suspected. LCM was used to retrieve gastric cells from the histologic slide, classified as gastric carcinoma, and suspected to be mislabelled. DNA was extracted from microdissected cells, and a total of 16 different genetic loci were analyzed, using an identity test. Comparison of the results with those obtained using DNA extracted from a control slide, and from patient's saliva, demonstrated a distinct DNA fingerprint pattern in all genetic markers examined, clearly indicating the occurrence of a specimen mix-up. The combined use of LCM and DNA fingerprinting represents the most accurate and sophisticated method available for the identification of specimen mix-up, especially when only the tissue on the suspected slide is available.     

Individual identification from semen by the deoxyribonucleic acid (DNA) fingerprint technique

For individual identification from semen, the deoxyribonucleic acid (DNA) fingerprint technique was used. In a blind trial, we succeeded in determining the semen donors among several volunteers comparing the DNA fingerprints of the blood and semen samples, respectively. Thereafter, we examined semen in a condom left beside a naked female dead body. The DNA fingerprint of the semen was recognized to be identical to that of the blood from a suspected man arrested later. This is the first report that the DNA fingerprint technique was practically used in a criminal investigation in Japan.     

Testing for Potential Contextual Bias Effects During the Verification Stage of the ACE‐V Methodology when Conducting Fingerprint Comparisons*

Abstract: This study was conducted to assess if fingerprint specialists could be influenced by extraneous contextual information during a verification process. Participants were separated into three groups: a control group (no contextual information was given), a low bias group (minimal contextual information was given in the form of a report prompting conclusions), and a high bias group (an internationally recognized fingerprint expert provided conclusions and case information to deceive this group into believing that it was his case and conclusions). A similar experiment was later conducted with laypersons. The results showed that fingerprint experts were influenced by contextual information during fingerprint comparisons, but not towards making errors. Instead, fingerprint experts under the biasing conditions provided significantly fewer definitive and erroneous conclusions than the control group. In contrast, the novice participants were more influenced by the bias conditions and did tend to make incorrect judgments, especially when prompted towards an incorrect response by the bias prompt.     

Fracture of the hyoid bone associated with atlantoaxial subluxation: a case report and review of the literature

Hyoid bone fractures secondary to blunt trauma other than strangulation are rare. Only 27 cases have been reported in the literature. They have few, if any, complications, such as dysphagia, hypoxia, cardiorespiratory collapse, laceration of the pharynx, mandibular fracture, thyroid cartilage fracture, and facial fracture. No report of hyoid bone fracture associated with spinal column injury was reported in the English literature. The authors reported a 46-year-old male patient who complained of neck pain and odynophagia after a traffic accident. After systemic examination and cervical CT scan, the diagnosis of hyoid bone fracture at the right great horn and atlantoaxial subluxation was made. The patient was observed in ICU for 48 hours. A nasogastric tube was inserted for feeding. Fifteen milligrams of dexamethasone was given once a day for 3 days to reduce the swelling and pain. We performed a cutaneous traction for the subluxation and carefully watched the hyoid bone and the patient's respiration. No lethal complications occurred. Two weeks later, the patient was allowed to ambulate with cervical collar protection and to resume oral intake. Three months later, he was asymptomatic. This case, with hyoid fracture and atlantoaxial subluxation, is the only case reported in the literature. The importance of hyoid fracture, however, rests not with the rarity of it, but with the lethal potential of missing diagnosis. Nonsurgical management may be effective in most of the cases.     

STR genotyping and mtDNA sequencing of latent fingerprint on paper

A systematic study was conducted to investigate whether DNA can be successfully extracted from latent fingerprints deposited on ordinary paper and analysed using short tandem repeat profiling and mitochondrial DNA sequencing. In order to evaluate the performance of latent fingerprint analysis in a criminal case, experiments with varying conditions were carried out to improve our understanding of low copy number (LCN) DNA typing. After optimising the extraction methods to achieve increased sensitivity, the examination of touched paper can routinely yield the STR profile of the individual who has touched it. A fingerprint can therefore be considered as a potential source of DNA for genetic identification. Nevertheless, the findings of our "after enhancement experiment" (using chemically or physically pre-treated fingerprints), and our "mixture experiment" (using fingerprints from three to four people on the same sheet of paper) help to define the limitations of the low copy number PCR technique in forensic casework.     

Pore Sub‐Features Reproducibility in Direct Microscopic and Livescan Images—Their Reliability in Personal Identification

Abstract: Third level features have been reported to have equal discriminatory power as second level details in establishing personal identification. Pore area, as an extended set third level sub‐feature, has been studied by minimizing possible factors that could affect pore size. The reproducibility of pore surface area has been studied using direct microscopic and 500 ppi Livescan images. Direct microscopic pore area measurements indicated that the day on which the pore area was measured had a significant impact on the measured pore area. Pore area measurement was shown to be difficult to estimate in 500 ppi Livescan measurements owing to lack of resolution. It is not possible to reliably use pore area as an identifying feature in fingerprint examination.     

Latent justice? A review of adversarial challenges to fingerprint evidence

This article provides an overview of recent research on latent fingerprint evidence featured in reported legal decisions from England and Wales, Australia and New Zealand. The research casts doubts on the effectiveness of adversarial criminal procedure. Rather, than engage with the methodological foundations – e.g. validity and reliability – and the actual abilities of fingerprint examiners, for more than a century, challenges were based on legal considerations and the meaning of categorical identification for the specific proceedings. Lawyers challenged fingerprint evidence based on the circumstances in which reference prints were collected, whether fingerprint records were hearsay, whether relying on a fingerprint record is unfair because it suggests prior criminality, whether the jurors could make their own comparison and so forth. There is no reported consideration of the validity and reliability of fingerprint comparison, and no requirement for fingerprint examiners to qualify the significance of a match decision, even after the abandonment of point standards and the appearance of critical reports from the United States and Scotland, and advice from the Forensic Science Regulator. To the extent that they considered the admissibility and probative value of this prominent forensic science evidence, lawyers and judges relied heavily on proxies such as training, experience and long use. In consequence, the article considers how we should understand adversarial legal practice, the performance of lawyers and judges, as well as the implications for forensic scientists and their evidence.     

Contextual information renders experts vulnerable to making erroneous identifications

We investigated whether experts can objectively focus on feature information in fingerprints without being misled by extraneous information, such as context. We took fingerprints that have previously been examined and assessed by latent print experts to make positive identification of suspects. Then we presented these same fingerprints again, to the same experts, but gave a context that suggested that they were a no-match, and hence the suspects could not be identified. Within this new context, most of the fingerprint experts made different judgements, thus contradicting their own previous identification decisions. Cognitive aspects involved in biometric identification can explain why experts are vulnerable to make erroneous identifications.     

The use of thoracolumbar and hip joint dysmorphism in identification

A case of roentgenographic identification of a deceased person is presented. Comparison of antemortem and postmortem radiographs of the vertebral column and hip joint, for the purpose of identification, was based on the deceased medical history, and the fact the deceased was endentulous, and fingerprint comparison was not feasible. Evaluation of anatomical variants, due to the effects of degenerative disease, provided positive identification in a visually unidentifiable deceased person.     

指印检验新方面

100多年来指印鉴定一直是犯罪侦查最有效的个体识别手段,在物证鉴定领域占据重要地位,上世纪80~90年代指印检验技术研究的重要进步更是使指印物证技术应用达到一个新的水平。最近几年指印技术研究又取得许多新进展和突破,特别是在指印显现技术和指印纹线物质成分分析方面开创了新方法和新方向,形成指印物证应用新体系,但同时在指印鉴定结论可靠性方面也出现新争议。     

对尸体指纹种类认定依据的研究

指纹状态属性的认定是当前指纹鉴定工作的一个新课题。指纹鉴定中的押名指印一般情况下都是押名人生前主动捺印形成的,但在实践中有时会出现作案人为了扰乱侦查视线、逃避惩罚或获取某种利益,而利用尸体指纹捺印伪造死者生前的押名指印的情况,因此对尸体指纹种类认定的研究将有助于侦查破案或对事实的确认。早期尸体现象是尸体指纹具备区别于活体指纹的特定性和相对稳定性的关键,再加上指纹本身所具有的反映性,这＂三性＂共同构成了尸体指纹种类认定的科学基础,为判定尸体指纹提供了理论依据。对于尸体指纹的研究,一方面须得益于尸体指纹捺印外观特征规律的深入总结,而另一方面也需要将微观层面的尸体指纹汗孔特征作为研究的切入点。总之,研究的最终目的就是提高尸体指纹种类认定的科学可靠性,进而使这一认定结论能作为一种科学的鉴定意见在诉讼中运用。     

指印印面特征的运用——CNAS-T18指印项目结果解析

通过对CNAS-T18指印项目实施与结果的解析,讨论了当前国内利用指印印面特征的现状：民事案件指印鉴定基础理论的不足。提出了对民事案件指印特征重新进行分类研究,以及探讨、规范利用指印印面特征步骤、方法的必要性和紧迫性。强调了指印印面特征在解决民事案件中的重要作用。     

Performance evaluation of automated fingerprint identification systems for specific conditions observed in casework using simulated fingermarks

Few studies have been reported on the performance evaluation of automated fingerprint identification systems (AFIS) for fingermark-to-fingerprint comparisons. This paper aims to illustrate to fingerprint examiners the relevance of evaluating the AFIS performance under specific conditions by carrying out five types of performance tests. The conditions addressed are the number of minutiae assigned to a fingermark, manual and automatic assignment of the minutiae, the finger region from which the fingermark originates, the degree of distortion in the fingermark, and the difference in orientation between fingermarks and fingerprints. In these tests, the magnitude of the influence for each condition was quantified. The comparisons were performed using a research AFIS technology with simulated fingermarks. Simulated fingermarks provide a practical way to create fingermarks for specific conditions in large quantities. The results showed that each condition influences the performance significantly, emphasizing the relevance of developing, and applying performance tests for specific conditions.     

Fingerprint error rate on close non‐matches

The accuracy of fingerprint identifications is critically important to the administration of criminal justice. Accuracy is challenging when two prints from different sources have many common features and few dissimilar features. Such print pairs, known as close non‐matches (CNMs), are increasingly likely to arise as ever‐growing databases are searched with greater frequency. In this study, 125 fingerprint agencies completed a mandatory proficiency test that included two pairs of CNMs. The false‐positive error rates on the two CNMs were 15.9% (17 out of 107, 95% C.I.: 9.5%, 24.2%) and 28.1% (27 out of 96, 95% C.I.: 19.4%, 38.2%), respectively. These CNM error rates are (a) inconsistent with the popular notion that fingerprint evidence is nearly infallible, and (b) larger than error rates reported in leading fingerprint studies. We conclude that, when the risk of CNMs is high, the probative value of a reported fingerprint identification may be severely diminished due to an elevated false‐positive error risk. We call for additional CNM research, including a replication and expansion of the present study using a representative selection of CNMs from database searches.     

Radiographic identification using the clavicle of an individual missing from the Vietnam conflict

A case is reported in which radiographic comparison of a clavicle was used to establish the identification of a civilian missing from the Vietnam conflict. While the use of radiographic comparison of skeletal features is not a newly developed technique for personal identification purposes, this case outlines a unique set of circumstances surrounding the disappearance of an individual during the Vietnam conflict and his eventual identification. A radiographic comparison of a right clavicle was critical in the identification process of this individual almost 34 years after he was reported missing. The use of digital technology for radiographic comparison greatly facilitated the process.     

Under the microscope: legal challenges to fingerprints and DNA as methods of forensic identification1

Suspects in legal cases can be identified by an ever‐growing list of novel methods. The most common techniques currently used include latent print and DNA analysis. Although standard fingerprinting entered the courtroom over a century ago, the admissibility of fingerprint evidence has undergone a period of intense scrutiny in the USA in recent years. In contrast, most challenges to DNA analysis as a science came during its inception in the late 1980s and early 1990s. Current challenges to fingerprint evidence attempt to discredit the science behind the theory whereas challenges to DNA evidence often bring into question the competency of the analyst. In either case, the lessons learned in various court systems give guidance for those implementing the newer emerging biometric identification technologies such as facial recognition systems, retinal scans and the like. The first section of this article deals with fingerprint analysis and recent challenges to fingerprint admissibility in US courts. The second section discusses the evolution of DNA analysis and relevant cases. The final section gives recommendations for emerging biometric technologies to follow to satisfy the standards set forth by the courts.