病毒性出血败血症病毒实时荧光RT-RPA恒温检测方法的建立

为建立一种简单、快速的病毒性出血败血症病毒(VHSV)检测方法,本研究通过比较分析VHSV N基因的保守序列,设计了多对引物和一条探针。经过筛选优化,建立了实时荧光RT-RPA检测方法。该方法在39℃恒温反应20 min即可快速、特异性地检测出VHSV,与其他水生动物病毒不发生交叉反应,该方法检测限为1.83×10^3PFU/mL,利用本研究方法对30份鲑鳟鱼临床样品以及9份鱼类疫病病原样品进行检测,检测结果与荧光定量PCR方法结果一致。上述结果表明,本研究建立的检测方法操作简单,反应灵敏,结果可靠,仪器依赖性低,适用于现场对VHSV快速检测。     

Deepening Understanding

<正>Cultural exchange activities increase mutual knowledge of China and Switzerland Before Chinese President Xi Jinping’s first visit to Switzerland as head of state in mid-January,a series of cultural exchange activities were held in the European country,including a book fair,a photo exhibition and a media dialogue session.     

论军事秩序与军事法的秩序价值

法律价值冲突是探讨法律价值时永恒不移的重点,许多学者认为基于军事与法的逻辑差别,价值冲突在军事法学领域表现得更为剧烈.因此,军事法律价值的冲突问题引发了众多争论,其中颇受重视的就是秩序价值.然而,现阶段的争论过于强调冲突本身,急于直接提供一种价值取向,但对于具体价值的内容思考不够深入,便很难达成一致意见.对军事法的秩序价值进行有针对性的探讨以及对军事秩序的概念进行解构应当能为解决军事法价值冲突问题提供一个全新的视角.     

An Uncertain Future

The Diaoyu Islands dispute continues to bring political and economic repercussions The incumbent Japanese Prime Minister Yoshihiko Noda retained leadership of the ruling Democratic Party of Japan (DPJ) in the latest DPJ presidential election in late September. Days later, after defeating four opponents,     

加强满意度调查 促进工会工作纵深发展——以某学校工会工作满意度调查研究为例

为了解工会会员对工会工作的满意度,促进工会工作进一步发展与提高,采用问卷调查法进行调查.调查发现群众对工会的满意度比较高,工会工作卓有成效;组织建设和文化建设工作突出,生活保障职能、维护职工权益和参与决策工作职能需加强;工会干部素质满意度评价很高,也需要进一步加强创新意识、政治法律素质、服务意识与协调能力的培养;工会活...     

The Coming Storm

正The Trump administration’s Jerusalem decision is of great consequence for the Middle East U.S.President Donald Trump’s recognition of Jerusalem as the capital of Israel,and the subsequent decision to relocate the U.S.embassy there from Tel Aviv in December 2017,invoked a tempest of criticism from around the world.     

新疆地区宗教形势与去极端化

宗教极端主义是滋生恐怖主义的重要原因之一。新疆特别是南疆地区,宗教氛围异常浓厚,为宗教极端思想的传播提供了有利的环境。宗教狂热的深层次原因必然是教育、就业、民生、文化、宗教等问题。因而,要从推行全面教育提高少数民族文化水平,提高就业率,规范宗教活动等方面改良滋生恐怖主义的土壤。     

Paltry Progress

The Doha climate conference extends the Kyoto Protocol,but evades issues of ensuring financing and shaping bigger ambitions The Kyoto Protocol has been extended by eight years and will come into effect as of January 1,2013.That’s where the good     

Classic Movie,New Success

正Titanic 3D,a retouched version of the 1997 Hollywood blockbuster,swept the Chinese box office by raking in 500 million yuan($79 million) in the first week(April 10-16) of its release,according to the China Film News. It is a new record high in terms of firstweekboxofficerevenueinChina.Moviegoers have to queue for hours to get a ticket.It took nine days for Transformers to hit this figure