H9亚型禽流感病毒RT-PCR检测方法的建立

通过分析流感数据库中123个HA序列,根据HA保守区序列设计并合成了1对引物,建立了一步法RT-PCR检测方法,预期扩增片段大小为732 bp。通过对H9亚型禽流感病毒(AIV)不同稀释度的尿囊液和棉拭子浸出液进行检测,证实病毒尿囊液的最低检出量为1×104.7EID50/mL;阳性棉拭子的最低检出量为1×102.5EID50/mL。用该方法检测H1~H15亚型AIV和鸡新城疫病毒等其他14种禽病病原,结果仅有H9亚型AIV出现特异性目的条带,而其他均未出现目的条带。脏器及咽喉、泄殖腔棉拭子样品在1∶10稀释度下,病毒分离和RT-PCR方法可以达到相同的阳性检出率。表明建立的AIV H9亚型RT-PCR方法具有较好的特异性和敏感性。     

Introducing the Open Clause to improve copyright flexibility in cyberspace? Analysis and commentary on the proposed “two-step test” in the Third Amendment to the Copyright Law of the PRC,in comparison with the EU and the US

The so called “three-step test”, that the limitations and exceptions of copyright shall be allowed in certain special cases, provided that they do not conflict with a normal exploitation of the work and do not unreasonably prejudice the legitimate interests of the author, grants copyright flexibilities to balance the interests of all stakeholders, especially within the European system of circumscribed limitations and exceptions. This is essential for the domain of computer law, confronted by rapid and unpredictable global technological developments, and is, thus, enshrined in the most important international intellectual property (IP) treaties. Through the proposed third amendment to the Copyright Law of the PRC, the legislature intends to adopt this test while also introducing an open-ended list of limitations and exceptions that constitutes a China-specific “two-step test.” This contravenes prima facie the thesis endorsed by the WTO Panel in the case concerning Section 110(5) of the US Copyright Act in 2000. In contrast, court decisions in China frequently apply the fair use doctrine of US copyright law, neglecting to consider its peculiar context of the US common law tradition and, thus, unduly expanding the Chinese courts' discretionary power.This paper summarizes the case law in China and takes a comparative approach to address the divergence between the judicial application of cyber copyright law and the existing legislation. It suggests revising the proposed Article 43 of the Copyright Law of the PRC to capture the due interpretation of the three-step test, thereby finessing the delineation between rights protection and free use with the compensation of remuneration under the principle of proportionality. It argues that transplanting the US fair use doctrine into Chinese copyright law is feasible, but with the preconditions of endeavouring to strengthen judicial reform to integrate the IP adjudication systems, enhancing the coherence and efficiency of copyright enforcement, and facilitating consistent dialogues between scholars, practitioners, and lawmakers.     

A Neoconservative Consensus in Russia?

This article analyzes the ideational consensus that has taken shape in contemporary Russian politics, including its key components, and factors both of stability and of potential erosion. Noting the extraordinary durability of this consensus, which is supported by factors including the duration of the regime itself, its support among elites and the bureaucracy, effective mobilizational propaganda and conservative orientations among the middle classes and dominant public opinion, the author draws attention to several factors – as yet less evident – that may in the future change the situation. Among these are the volatility of public opinion, potential intra-elite dynamics, and the internal contradictions of the neo-conservative idea itself.     

On Individual Business System in Normal Market

Individual business is a business entity system created in the practice of reform and opening up in China. It is the largest market entity in China, and its vitality lies in the "family culture" characteristics of Chinese society. Although individual businesses have been confirmed by law at all levels, they still have transitional and non-normative doubts. In some places, individual businesses have been transformed into enterprises. Individual businesses take advantage of the natural credit bond, low cost of organization and coordination, and are targeted to meet the needs of the market, which is conducive to absorbing employment and balancing wealth, easy to disperse and digest business risks, and has a competitive advantage in the low-end market. In a normal market, the rational choice of the parties concerned should be respected, the hierarchical structure of the market should be adapted, the complete ecology of market subjects should be maintained, and the reform of individual enterprises should be prudently implemented.     

Potency Trends of Δ9‐THC and Other Cannabinoids in Confiscated Cannabis Preparations from 1993 to 2008*

Abstract: The University of Mississippi has a contract with the National Institute on Drug Abuse (NIDA) to carry out a variety of research activities dealing with cannabis, including the Potency Monitoring (PM) program, which provides analytical potency data on cannabis preparations confiscated in the United States. This report provides data on 46,211 samples seized and analyzed by gas chromatography‐flame ionization detection (GC‐FID) during 1993–2008. The data showed an upward trend in the mean Δ⁹‐tetrahydrocannabinol (Δ⁹‐THC) content of all confiscated cannabis preparations, which increased from 3.4% in 1993 to 8.8% in 2008. Hashish potencies did not increase consistently during this period; however, the mean yearly potency varied from 2.5–9.2% (1993–2003) to 12.0–29.3% (2004–2008). Hash oil potencies also varied considerably during this period (16.8 ± 16.3%). The increase in cannabis preparation potency is mainly due to the increase in the potency of nondomestic versus domestic samples.     

高等学校“概论”课开放式教学方式探索——“听、读、评、写、研”教学模式实践及启示

在“毛泽东思想和中国特色社会主义理论体系概论”课程教学中开展“听、说、读、评、研”教学活动,是深化教学改革,培养创新型人才的可行途径之一。在教学中结合课程内容让学生读一些相关的书目,搞一些同学们有兴趣又能参与其中的活动,使同学们在了解历史但又不脱离现实生活的状态下,既掌握了知识又活跃了课堂气氛,提高了同学们的理论素养以及分析问题和解决问题的能力,从而坚定在中国共产党的领导下走中国特色社会主义道路的理想信念。     

猪繁殖与呼吸综合征病毒重组质粒pEGFP-NSP9的构建与表达

为验证siRNAs对猪繁殖与呼吸综合征病毒复制的抑制效果,构建了猪繁殖与呼吸综合征病毒NSP9基因与增强型绿色荧光蛋白基因GFP的融合表达质粒,并在Marc145细胞中进行了表达。通过RT-PCR方法扩增猪繁殖与呼吸综合征病毒NSP9基因,将其克隆入表达载体pEGFP-N1,并进行双酶切、PCR及测序鉴定。将阳性重组质粒转染Marc145细胞,检测绿色荧光蛋白的表达和NSP9基因转录水平。结果显示,经双酶切及PCR鉴定,目的基因的大小与预期相符。荧光显微镜和流式细胞仪均检测到细胞内绿色荧光蛋白的表达,荧光定量PCR检测到细胞内有NSP9基因的转录。本研究成功构建的猪繁殖与呼吸综合征病毒NSP9基因GFP融合表达质粒,为在细胞水平快速筛选有效的siRNAs提供了工具。     

三株鸭源H9N2亚型禽流感病毒福建株全基因的测序及遗传进化分析

为明确3株鸭源H9N2亚型禽流感病毒福建株A/Muscovy duck/Fujian/CL/1997(H9N2)(简称CL株)、A/duck/Fujian/MH/2003(H9N2)(简称MH株)和A/duck/Fujian/FQ2/2007(H9N2)(简称FQ2株)全基因组的分子特征和遗传进化情况,采用RT-PCR方法扩增这3株病毒全基因片段,并进行序列比较及遗传进化分析。结果表明,所克隆的8个基因片段均有完整的开放阅读框,其血凝素基因蛋白裂解位点的氨基酸组成为-RSSR*GLF-,为不连续的碱性氨基酸,其静脉接种指数远小于1.2,均符合低致病性AIVHA蛋白裂解位点特征;CL株和MH株的HA基因在遗传进化上属于常见的CK/BJ/1/94群系;FQ2株HA基因在遗传进化上属于DK/HK/Y280/97群系,3株H9N2亚型AIV HA基因在遗传进化上均属于经典的欧亚种系。3株H9N2亚型AIV NA基因颈部均存在缺失,CL株和MH株的NA基因在遗传进化上属于常见的CK/BJ/1/94群系,FQ2株NA基因在遗传进化上属于G1群系。CL株和MH株的其他6个基因在遗传进化上均和经典的CK/BJ/1/94一致,而FQ2株则可能是由不同流感病毒基因亚系间发生自然重排的产物。     

Arab Muslim Attitudes Toward the West: Cultural,Social, and Political Explanations

We utilize pooled data from Zogby International's 2002 Zogby, James. 2002. What Arabs Think: Values Beliefs and Concerns, Utica NY: Zogby International.  [Google Scholar] Arab Values Survey (carried out in Egypt, Jordan, Lebanon, Kuwait, Morocco, Saudi Arabia, and UAE) in order to test for “cultural,” “social” and/or international “political” influences on Arab Muslim attitudes toward “Western” countries (Canada, France, Germany, UK, and USA). We find little support for “cultural” hypotheses to the effect that hostility to the West is a mark-up on Muslim and/or Arab identity. We find only limited support for “social” hypotheses that suggest that hostility to the West is predicted by socioeconomic deprivation, youth, and/or being male. We find the strongest support for a lone “political” hypothesis: hostility toward specific Western countries is predicted by those countries' recent and visible international political actions in regard to salient international issues (e.g., Western foreign policies toward Palestine).     

Evaluating Claims of Social Connection to International Conflict Casualties

Recent studies examining the political impact of individuals' connections to the victims of international violence find these ties have a powerful effect on people's attitudes and feelings. How reliable, however, are self-reported claims of ties to a conflict's casualties? Using data from 9/11 and the Iraq War, I examine these claims, analyzing: 1) their influence on both public assessments of foreign policy and voting behavior, 2) whether critical demographic and political factors predict the likelihood of individuals reporting a tie to a conflict casualty, 3) the predicted, aggregate likelihood of survey respondents having connections to conflict victims, and 4) the theoretical distinction between actual vs. perceived casualty connections. The results strongly support the use of casualty connection data for understanding individuals' responses to international violence, and encourage future applications of social network approaches to the study of war and politics.