中华鳖TLR2部分序列的克隆及其组织表达差异分析

为探究中华鳖天然免疫的分子机制,针对TLR2(Toll-like receptor 2)保守区设计兼并引物,从中华鳖脾cDNA中扩增出了740bp的目标序列。测序和序列分析结果表明,该序列包含富含半胱氨酸型C端结构域、跨膜结构域和TIR结构域;与斑马鱼、鸡等脊椎动物相应的TLR2序列相似性为60.5%～74.4%。采用荧光定量PCR检测中华鳖感染嗜水气单胞菌后各组织TLR2mRNA表达的变化,结果显示,感染后第24小时,中华鳖脾和外周血TLR2mRNA相对表达水平升高,其中外周血中的增幅最显著,是对照组的8.57倍;中华鳖外周血经4μg/mL酵母聚糖刺激后,第2小时TLR2mRNA相对表达水平较高。首次获得了中华鳖TLR2部分cDNA序列,并分析了不同刺激物对中华鳖TLR2mRNA转录的影响。     

三株鸭源H9N2亚型禽流感病毒福建株全基因的测序及遗传进化分析

为明确3株鸭源H9N2亚型禽流感病毒福建株A/Muscovy duck/Fujian/CL/1997(H9N2)(简称CL株)、A/duck/Fujian/MH/2003(H9N2)(简称MH株)和A/duck/Fujian/FQ2/2007(H9N2)(简称FQ2株)全基因组的分子特征和遗传进化情况,采用RT-PCR方法扩增这3株病毒全基因片段,并进行序列比较及遗传进化分析。结果表明,所克隆的8个基因片段均有完整的开放阅读框,其血凝素基因蛋白裂解位点的氨基酸组成为-RSSR*GLF-,为不连续的碱性氨基酸,其静脉接种指数远小于1.2,均符合低致病性AIVHA蛋白裂解位点特征;CL株和MH株的HA基因在遗传进化上属于常见的CK/BJ/1/94群系;FQ2株HA基因在遗传进化上属于DK/HK/Y280/97群系,3株H9N2亚型AIV HA基因在遗传进化上均属于经典的欧亚种系。3株H9N2亚型AIV NA基因颈部均存在缺失,CL株和MH株的NA基因在遗传进化上属于常见的CK/BJ/1/94群系,FQ2株NA基因在遗传进化上属于G1群系。CL株和MH株的其他6个基因在遗传进化上均和经典的CK/BJ/1/94一致,而FQ2株则可能是由不同流感病毒基因亚系间发生自然重排的产物。     

Reduced payer's liability for unauthorized payment transactions under the second Payment Services Directive (PSD2)

In 2015, the European legislator enacted the second Payment Services Directive, which, among other things, determines the payer's liability for unauthorized payment transactions. This paper will show that PSD2 has widened its scope and further reduces the payer's liability for unauthorized transactions, for example by making it impossible to hold the payer liable in case no strong customer authentication has been applied, by requiring supporting evidence to prove the payer's fraud or gross negligence and by applying the same rules when unauthorized transactions are initiated through a payment initiation service.     

Risk-Need-Responsivity applications of the MMPI-2 in sexual offender assessment

The present investigation examined the risk, need, and responsivity (RNR) correlates of MMPI-2 scores in a Canadian sample of 349 federally incarcerated sex offenders, followed up prospectively 19 years post-release. In terms of responsivity indicators, more serious profile patterns were associated with younger age, single marital status, lower education, and lower cognitive ability; the scales generally had weak associations with sex offender treatment completion or change. With respect to criminogenic risk and need, Scales F, 4, 6, 8, and 9 and combinations therein had significant associations with structured measures of sex offender risk, and in turn, consistently predicted sexual or violent recidivism over 5 and 10-year follow-ups. Several of these predictive associations were maintained even after controlling for static and dynamic risk factors. Finally, model based clustering of the MMPI-2 scales generated three clusters termed disordered, emotionally distressed, and predominantly antisocial (non-disordered) subtypes. Although comparatively higher rates of violent recidivism were found with the disordered subtype, this group did not have higher levels of risk and need, broadly speaking, than the other subtypes. The RNR implications of the results are discussed in terms of forensic applications of MMPI-2 with sex offender populations.     

Opinion 1/15 of the Grand Chamber dated 26 July 2017 about the agreement on Passenger Name Record data between the EU and Canada

On 26 July 2017, the Grand Chamber of the European Court of Justice rendered its seminal Opinion 1/15 about the agreement on Passenger Name Record data between the EU and Canada. The Grand Chamber considered that the decision of the Council about the conclusion, on behalf of the Union, of the agreement between the EU and Canada about the transfer and processing of PNR data must be based jointly on Article 16(2) about the protection of personal data and Article 87(2)(a) about police co-operation among member states in criminal matters, but not on Article 82(1)(d) about judicial co-operation in criminal matters in the EU of the Treaty on the Functioning of the EU. The Grand Chamber also considered that the agreement is incompatible with Article 7 on the right to respect for private life, Article 8 on the right to the protection of personal data, Article 21 on non-discrimination and Article 52(1) on the principle of proportionality of the Charter of Fundamental Rights of the EU since it does not preclude the transfer, use and retention of sensitive data. In addition to the requirement to exclude such data, the Grand Chamber listed seven requirements that the agreement must include, specify, limit or guarantee to be compatible with the Charter.The opinion of the Grand Chamber has far-reaching implications for the agreement on PNR data between the EU and Canada. It has also far-reaching implications for international agreements on PNR data between the EU and other third states. Last, it has far-reaching implications for Directive 681 of 27 April 2016 on PNR data.     

毓麟2号方对男性不育伴精索静脉曲张患者精液参数及精子DNA碎片指数的影响

目的 观察毓麟2号方对肾虚瘀阻型男性不育伴精索静脉曲张患者精液参数及精子DNA碎片指数(DNA-fragmetation index,DFI)的影响.方法 将118例肾虚瘀阻型男性不育伴精索静脉曲张患者随机分为治疗组和对照组,每组59例.治疗组给予毓麟2号方,对照组给予迈之灵片联合五子衍宗丸.比较两组患者临床综合疗效和...     

Protection gaps for civilian victims of political violence

This article begins by explaining why the United Nations' civilian protection agenda is particularly relevant and important for Africa and why the Responsibility to Protect (R2P) might be said to be an African norm export to the rest of the world. Next, it traces the reasons for peace operations' reluctance to use force. It then shows how the civilian protection agenda has tried to fill critical gaps in the existing normative architecture, with both R2P and the Protection of Civilians (POC) resulting from growing shame at the accumulating list of atrocities in which the international community stood by as passive onlookers, frustrations at the ‘constitutional’ constraints and normative inadequacies rather than indifference and apathy to the plight of civilian victims that produced the passivity, and a determination to reposition the United Nations system to be empowered and capacitated to be able to respond better on both the timeliness and effectiveness dimensions when confronted by repeat occurrences of similar tragedies. The fourth section discusses the merits of the R2P and POC norms in responding to the challenge of civilian protection. The final part notes that, despite these two valuable additions to the repertoire of the international community in dealing with atrocities perpetrated on civilians, there remain many gaps in the protection agenda, as shown in several recent cases.     

RyR2相关心脏性猝死的研究进展

雷诺定受体2(ryanodine receptor 2,RyR2)是心肌细胞肌浆网上主要的钙释放通道,主要参与心肌的兴奋收缩耦联过程,其基因突变及调控异常可导致心肌舒张期钙漏引起心律失常,甚至猝死。本文重点综述RyR2基因突变及调控异常引起心脏性猝死的机制,并对以后的研究提出建议,旨在为心脏性猝死的法医学诊断提供一种新的思路。     

COOPERATIVES FOR THE PERFORMING ARTS: A PROSPECTUS

The author describes research that addresses the problem of organizational change associated with the use of new information and communication technologies. The author focuses on the change processes associated with the use of the World Wide Web by cultural heritage organizations: archives, galleries, libraries, and museums. This research note outlines the research problem and the theoretical perspectives and research methodologies to be used in the research.     

Internal reliability and construct validity of the Novaco Anger Scale-1998-S in a sample of violent prison inmates in Sweden

Abstract

In this study, the internal reliability and construct validity of the recently adapted Swedish version of the Novaco Anger Scale (NAS-1998-S; Lindqvist, Dåderman, & Hellström, Social Behavior and Personality, 8, 773–788, 2003), as well as its scale correlations with demographic and criminality variables, were investigated. Construct validity was established by assessing the correlation pattern of the scales of NAS-1998-S with concurrent scales of similar and distinct constructs. Ninety-five male violent prisoners, ranging in age from 18 to 67 years, participated. The results demonstrated good internal reliability, consistent intrascale relationships, and appropriate construct validity of NAS-1998-S. The number of previous convictions had a moderate negative relationship with the capacity of control. Age and education correlated negatively with the NAS-1998-S scales, except Regulation. In addition to psychometric issues, the results were discussed from a clinical perspective on the offender population.