益化清解方对2型糖尿病大鼠炎性分子网络的影响

[HT5K]目的:观察益化清解方对2型糖尿病（T2DM）大鼠炎性分子网络调控的影响。方法:复制T2DM大鼠模型,治疗组用益化清解方中药灌胃,并与模型组、二甲双胍组及正常组作对照。分别在给药前和给药第15,30天检测T2DM大鼠的肿瘤坏死因子 α（TNF α）、白介素6(IL 6）、高敏C 反应蛋白（hsCRP）、脂联素(Adiponectin,Adp)、瘦素(leptin）水平,探讨其变化与空腹血糖（FBG）、血清胰岛素（FINS）及胰岛素敏感性指数（ISI）的关系;并检测各组大鼠脂肪组织脂联素及骨骼肌组织脂联素受体R1 Mrna表达水平,探讨Adp、Adp受体与这些因素之间的关系。结果:益化清解方能显著降低T2DM大鼠血糖、血脂、C反应蛋白等水平,并提高胰岛素敏感性,改善糖尿病大鼠的脂肪组织Adp Mrna表达水平。结论:益化清解方对糖、脂双调节的作用可能是通过增加胰岛素敏感性而实现的,其与改善糖尿病大鼠的脂肪组织Adp Mrna表达相关.     

H9N2亚型禽流感病毒聚合酶基因的克隆及序列分析

采用RT-PCR方法对1株H9N2亚型禽流感病毒Ck/GS/2/99株RNA聚合酶基因PB2、PB1和PA分别进行了扩增,将其克隆到pGEM-T easy载体后进行序列测定与分析。结果表明,该株病毒的PB2、PB1和PA基因开放阅读框(ORF)分别由2280、2274和2 151个碱基组成,分别编码759、757和716个氨基酸。经同源性与系统发生树分析,该毒株RNA聚合酶基因PB2、PB1和PA与Ck/NX/4/99株的核苷酸和氨基酸同源性均在98%以上,另外这3个基因与Dk/HK/Y280/97、Sw/HK/9/98和Qu/HK/NT/28/99株的关系密切。     

Genetic polymorphism at 15 tetrameric short tandem repeat loci in four aboriginal tribal populations of Bengal

POPULATIONS: This study reports the genetic polymorphism observed at 15 short tandem repeat loci D3S1358, TH01, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, CSF1PO, vWA, D8S1179, TPOX, D2S1338, D19S433, and FGA in four aboriginal populations of Bengal. The analysis was performed to decipher the suitability of CODIS as well as six other highly polymorphic and unlinked markers in Forensic Testing. Studied populations include four tribes: Karmali, Kora, Maheli, and Lodha.     

“9＋2”与“10＋1”框架机制比较

中国—东盟的全面经济合作,是应经济全球化而产生的国际区域合作的又一新的成果,其“合作框架协议”是最基本的法制依据。而我国南方九省区加上香港、澳门两地的“泛珠三角区域合作”则是在一个国家之内,“一国两制”之下不同省区横向合作的机制创新,它不仅受到了国际区域经济合作创新的启示,而且顺应了整个社会区域整体发展的潮流。本文将在二者的实施框架机制方面进行一定的比较,欲为“泛珠三角区域合作”的法制建构献可替否。     

H2O2诱导PC12细胞凋亡前后miRNA的变化及探讨

目的用微小RNA(miRNA)基因芯片技术检测H2O2诱导凋亡的PC12细胞和正常PC12细胞miR-NA的表达谱差异。方法分别以不同浓度的H2O2处理PC12细胞12h,用MTT和流式细胞仪检测处理后细胞的生长活力和凋亡情况;分别提取A(0浓度H2O2处理组)和B(400nmol/LH2O2处理组)PC12细胞的miRNA做miRNA基因芯片检测。结果以A组作为对照,30、50、100、200、400nmol/L的H2O2处理的PC12细胞生存率分别为(92±9.80)%、(90±14.70)%、(80±13.85)%、(54±12.33)%、(22±7.35)%(P<0.01);0、30、50、100、200和400nmol/L的H2O2处理的PC12细胞早期凋亡率分别为2.6%、5.2%、7.2%、10.4%、16.6%、72.2%;在样品A中共筛选出68个有效表达的miRNA分子数据,在样品B中筛选出46个有效表达的miRNA分子数据,两者样品中均检测到有效表达的miRNA分子有39个,其中与样品A相比,在样品B中显著性下调表达的有6个。结论为脑缺血再灌注损伤中神经细胞凋亡的机制和治疗的研究提供了理论依据和新的研究思路。     

二醋吗啡对大鼠心肌细胞游离Ca^2＋浓度的作用

目的观察二醋吗啡对心肌细胞内游离Ca2 浓度的作用。方法取自1～3d的SD大鼠培养的心肌细胞,用荧光探针Fluo-3/AM负载心肌细胞,不同浓度及不同剂量的二醋吗啡作用心肌细胞,激光共聚焦显微镜下检测Ca2 的变化。结果不同剂量及浓度的二醋吗啡对心肌细胞内游离Ca2 浓度有不同作用,一定浓度的二醋吗啡使心肌细胞内游离Ca2 浓度呈剂量依赖性的升高、短时间内心肌细胞[Ca2 ]i荧光强度增强,并产生[Ca2 ]i峰。结论探索出二醋吗啡导致心肌细胞内Ca2 变化的有效浓度,为进一步深入研究打下了基础。     

Comparison of two methods for isolating DNA from human skeletal remains for STR analysis

Abstract: The quality and efficiency of a standard organic DNA isolation method and a silica‐based method using the QIAGEN Blood Maxi Kit were compared to obtain human DNA and short tandem repeats (STRs) profiles from 39 exhumed bone samples for paternity testing. DNA samples were quantified by real‐time PCR, and STR profiles were obtained using the AmpFlSTR^® Identifiler^® PCR amplification kit. Overall, the silica‐based method recovered less DNA ranging from 0 to 147.7 ng/g (average 7.57 ng/g, median = 1.3 ng/g) than did the organic method ranging from 0 to 605 ng/g (average 44.27 ng/g, median = 5.8 ng/g). Complete profiles (16/16 loci tested) were obtained from 37/39 samples (95%) using the organic method and from 9/39 samples (23%) with the silica‐based method. Compared with a standard organic DNA isolation method, our results indicate that the published silica‐based method does not improve neither the quality nor the quantity of DNA for STR profiling.     

Determination of Acetamiprid and IM‐1‐2 in PostMortem Human Blood,Liver, Stomach Contents by HPLC‐DAD

An HPLC‐DAD method was developed to detect and quantify a neonicotinoid insecticide acetamiprid (ATP) and its metabolite IM‐1‐2 in autopsy samples of a fatal intoxication case. The postmortem blood and tissue distribution of ATP and IM‐1‐2 was determined for the first time. The method showed acceptable precisions and recoveries with relative standard deviations of <10% for ATP level and 1.38 % for IM‐1‐2. The detection and quantification limits for ATP were 0.015 μg/mL and 0.030 μg/mL for blood and were 0.035 μg/g and 0.050 μg/g for liver samples, respectively. The mean contents of ATP were 0.79 μg/g in the liver, 47.35 μg/g in the stomach contents and 2.7 μg/mL in the blood. IM‐1‐2 content was 17.0 μg/g in the stomach contents. ATP and IM‐1‐2 were not detected in the urine. The presence of ATP and IM‐1‐2 in the samples was confirmed by GC‐MS. The method can be exploited in future forensic casework.