植物栽培：壮泰族群同源与分化的佐证

植物的同名是共同族群认知某种植物的结果,是辨别民族起源是否同源的一个参考因素。壮泰族群对蕹菜、白菜、芋头等等本地植物的同样命名,是他们为＂同根生民族＂的又一证据。他们对烟草、玉米、红薯等外来植物的异样命名,说明他们由同源而分化了。泰族、佬族对白菜与芥菜的不区分,说明他们在公元6世纪就已经离开了中国的岭南地区。     

植物酶在解剖组织中有机磷、氨基甲酸酯农药检测中的应用

本文报导了有机磷和氨基甲酸酯检测的一个新的酶抑制法.该法基于有机磷和氨基甲酸酯化合物对植物酶水解底物2,6-二氯乙酰靛酚的抑制作用.本法简单,快速,适于农药中毒的现场检验.其最小检出量分别为0.2～1μg/μ1和1～2μg/ml.     

我国植物新品种行政执法制度的缺陷与完善

我国植物新品种行政执法制度中,存在行政裁决和行政处罚糅杂不清、假种子行政处罚代替品种权行政处罚的现象,影响品种权行政执法功能的发挥。从制度发展的总体走向来看,品种权行政裁决的功能在逐步弱化,而行政处罚日益成为执法重心。理清行政裁决和行政处罚的关系,强化品种权行政处罚制度,明确行政权介入的公共利益门槛,着重打击假冒、套牌等严重损害种业创新和市场秩序的行为,能使行政执法在种业知识产权保护上发挥更大的作用。     

论遗传资源的获取和惠益分享的产生

遗传资源是对人类具有实际或潜在用途或价值的一类重要的生物资源,遗传资源的获取问题经历了从自由获取向限制获取的转变过程。《生物多样性公约》的通过,标志着遗传资源的获取和惠益分享国际法律规范的正式确立,而《粮食和农业植物遗传资源国际条约》则针对粮食和农业植物遗传资源的特殊性质建立了粮食和农业植物遗传资源的获取和惠益分享多边系统。     

植物新品种临时保护请求权性质与规范构造

植物新品种临时保护请求权旨在保护育种者自提出或公告品种权申请至授权期间的利益,其性质应属不当得利返还请求权。该请求权的主体为品种权人及利害关系人,其行使应满足时间、行为、实际通知等条件。临时保护请求权的效力范围为请求行为人支付适当使用费,该使用费应以许可费为基础,酌情考虑个案因素加以确定。我国植物新品种临时保护请求权存在权利性质含糊、主体范围狭窄、行使条件不具体及效力范围不明确等问题,应结合UPOV1991文本、其他国家和地区的相关规定及我国司法实践加以完善,以提高我国植物新品种保护水平。     

Suicidal yew ingestion

A 41-year-old man was found dead, lying in prone position on the ground near a parking area, where the car of the person was parked. Remarkable were the spasm-like hands of the decedent, cause of death was unknown. Primary an epileptical attack was assumed. A medicolegal autopsy was performed followed by histological and forensic toxicological analysis. Furthermore a plant anatomical investigation was applied. Autopsy revealed fragments of greenish needle-like leaves in the stomach and the intestines, but not in the colon. Plant anatomical investigation indicated the presence of yew. Histological findings were in concordance with the literature, in addition the investigation of bone marrow was performed for the first time. Forensic toxicological analysis revealed the presence of 3,5-dimethoxyphenol, the marker for intoxication with taxus baccata.This case reports the importance of medicolegal examinations in order to avoid misdiagnosis. Here the suspection of an epileptical attack as a possible cause of death was excluded by an autopsy, in addition an intoxication by yew leaves was proved.     

二氯丙基酯化-气相色谱法分析葡萄叶中3种除草剂

目的建立葡萄叶中2甲4氯、2,4-滴和2,4-滴丙酸3种苯氧羧酸类除草剂的二氯丙基酯化-电子俘获检测气相色谱分析法。方法样品捣碎、添加内标2,4-二氯苯乙酸、酸化、用乙醚提取,提取液用液液分配法净化,净化后挥干有机溶液,用硫酸和1,3-二氯丙-2-醇进行酯化,生成的酯进行气相色谱分析。结果葡萄叶中2甲4氯、2,4-滴和2,4-滴丙酸的检测限分别为20ng/g、20ng/g和30ng/g,葡萄叶中除草剂浓度100~1000 ng/g成线性关系,空白葡萄叶中添加2甲4氯、2,4-滴和2,4-滴丙酸200ng/g的平均回收率分别为98.8%、101.3%和103.1%,回收率的变异系数分别为11.4%、11.0%和9.3%。结论本文方法检测被毒害的葡萄叶中3种除草剂是灵敏和准确的。     

Evaluation of Two New Methods for DNA Extraction of “Legal High” Plant Species

A quick, simple, and high-yield nucleic acid isolation process is crucial for high-quality DNA analysis. The ability of the MicroGEM PDQeX phytoGEM system and Omega Bio-tek E.Z.N.A.® Plant DS Mini kit to extract PCR-ready DNA was evaluated by extracting the forensically relevant “legal high” plant species: Ipomoea purpurea, Artemisia absinthium, Mitragyna speciosa, Datura stramonium, and Papaver somniferum. The plant material was pulverized, processed using the manufacturer’s plant protocol for the PDQeX Nucleic Acid Extraction or the manufacturer’s protocol for the Omega extraction, quantified using the Invitrogen Qubit 2.0 Fluorometer, and analyzed for amplifiability by PCR using a Qiagen Rotor-Gene Q instrument and published assays. The DNA amplicons for the legal high species produced high-resolution melt curves concordant with the melts observed when DNA was isolated using the Qiagen DNeasy Plant Mini Kit in previous studies.     

赛博女性主义述评

"赛博女性主义"这一提法出现于20世纪90年代初期,用来指赛博空间中女性之间强有力的互动关系及带有女性主义特点的活动。它大致可以分为两个分支:一是多纳.哈洛维(Donna Haraway)倡导的电子人隐喻,二是以塞迪.普朗特(Sadie Plant)为代表的关于女性与电子技术之间的关系的主张。本文试图评述赛博女性主义的理论要点和发展状况,剖析这一在90年代活跃一时的理论热点在进入新千年之后逐渐冷却的原因,认为赛博女性主义对我们今天了解社会性别和科学技术的关系具有积极的意义,而且在男权意识形态仍然占据主导地位的赛博文化中,赛博女性主义的呼声仍然十分必要。