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Ludovit Schreiber R.N.Dr. Martin Morovič Ph.D. Katarína Špacayová D.V.M. Radoslav Halko Ph.D. 《Journal of forensic sciences》2019,64(4):1274-1280
A case of suspected acute and lethal intoxication caused by colchicine has been reported. The woman was hospitalized after her suspicion of suicidal poisoning by a rare autumn crocus (Colchicum autumnale). Suspected colchicine poisoning was confirmed using a novel UHPLC method with a modern reversed‐phase stationary phase with a sub 2‐micron superficial porous particle size combined with a QTOF mass spectrometer. Sample preparation procedure included the addition of propiverine as internal standard, protein precipitation using methanol and solid phase extraction. High‐resolution MS only and targeted MS/MS modes are reported for the qualitative analysis and screening of other potential drugs of abuse in blood samples. All Ion MS mode was used for quantitative determination of colchicine afterward. The concentration of colchicine in the blood sample was approximately 41 ng/mL, and more than 200 μg/mL of the plant extract used for the suicide. 相似文献
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Petar Škavić M.D. Zijad Duraković M.D. Ph.D. Marina Nestić Ph.D. 《Journal of forensic sciences》2018,63(1):321-325
This study presents two cases of lethal bentazone poisonings, their clinical presentation, the course of the disease and the autopsy findings. The first is a 50‐year‐old male who had sprayed corn with a solution of bentazone and was admitted to the hospital with sweating, fever, nausea, vomiting of aqueous and hemorrhagic content, and bloody, watery stools. He was treated according to the symptoms including extracorporeal hemodialysis, but eventually suffered from multiorgan failure (acute respiratory failure, acute liver failure, coagulopathy, acute renal failure, metabolic acidosis, and gastrointestinal bleeding) and died 11.35 h after admittance. The cause of death was probable bentazone intoxication. The second case, also a male, aged 49 who committed suicide by ingesting a bentazone solution. He was transferred to the hospital prostrated and cyanotic and died 14.15 h after admittance despite all efforts by the hospital staff. The cause of death was acute bentazone intoxication. 相似文献
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1案例资料简要案情2010年01月20日,某市公安分局刑侦支队接到受害人王某报案称:2009年12月20日与其前女友张某一起喝茶后出现恶心、呕吐、腹痛、腹泻等症状,遂至当地人民医院对症治疗,消化道症状减轻; 相似文献
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Sebastien Prat M.D. Guillaume Hoizey Ph.D. Thierry Lefrancq M.D. Pauline Saint‐Martin M.D. Ph.D. 《Journal of forensic sciences》2015,60(3):816-817
Strychnine‐related death has been described since the 19th century. This alkaloid was discovered in 1818. Historically, strychnine was used by the South‐East Asian autochthones on their arrows. However, its production was modified by legislation, which was used to protect people against accidental intoxications. Here, we present the case of a 69‐year‐old man who was found dead at home. During the autopsy, we found a blue substance in the stomach. Toxicological analysis measured strychnine at 0.29 μg/mL in the blood sample, which is a relatively low level in comparison with the results given in the literature. However, histologic examination and toxicological findings permitted the conclusion of strychnine poisoning. 相似文献
37.
Hiroto Kawashima Ph.D. 《Journal of forensic sciences》2015,60(5):1360-1364
Between December 2007 and January 2008, people suffered from food poisoning in the Japanese prefectures of Chiba and Hyogo after eating frozen dumplings (gyoza) produced in China, which had very high concentrations (1490–19,290 ppm) of methamidophos (O,S‐dimethyl phosphoramidothioate). Thus, we measured the stable carbon isotope ratio of methamidophos using GC/C/IRMS to identify the source. We analyzed seven methamidophos reagents and one Chinese agricultural methamidophos chemical (MTD600) that contained many impurities. The δ13C values of the seven methamidophos reagents and MTD600 ranged from ?49.23‰ to ?31.90‰, with an average SD of 0.20‰, very high precision. This difference (17.33‰) was very large compared with that in previous reports and may be attributable to the material itself and the chemical processing of methamidophos. Criminals can easily obtain pesticides such as methamidophos; therefore, it is very important to identify the pesticide source and distribution route using stable isotopic science in the future. 相似文献
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Vannessa Lawai M.S. Nor Aliza Abdul Rahim Ph.D. Zainab Ngaini Ph.D. 《Journal of forensic sciences》2015,60(6):1620-1624
Paraquat poisoning is commonly associated with suicide or homicide in Malaysia. In a case involving advanced body decomposition, pathological analysis regarding the cause of death may become difficult or almost impossible. Insects serve as common alternative matrix for poison detection in forensic analysis. Paraquat detection via secondary bioaccumulation in fly larvae tissue has never been reported. In this study, tissues from blowfly larvae collected from a rabbit carcass with paraquat poisoning were analyzed for secondary bioaccumulation. Larvae samples were collected and analyzed using liquid–liquid extraction. The detection was performed via reduction of quaternary ammonium presence in paraquat and analyzed using gas chromatography–mass spectrometry (GC-MS) with selected ion monitoring mode (SIM mode). GC-MS showed the elution of reduced paraquat was at retention time 12.8 min. Blowfly larvae tissue has proven useful as a secondary detector in paraquat-related deaths. 相似文献
39.
生物样品中的金属毒物电感耦合等离子发射光谱检验 总被引:1,自引:0,他引:1
目的建立有毒金属元素在肝、脾和肾脏组织中的快速检测方法。方法通过模拟样品,采用微波消解炉对样品进行预处理,用电感耦合等离子发射光谱仪同时对多种可疑有毒金属元素进行检测。结果在中毒剂量,对肝脏和肾脏组织中的可疑毒金属元素,可以采用电感耦合等离子发射光谱仪进行检测。 相似文献
40.
目的研究慢性乙醇中毒引起小鼠脑神经细胞Ⅰ型1,4,5-三磷酸肌醇受体(IP3R1)表达的变化。方法 40只小鼠随机分为90d、180d组,各组再分为正常对照组、10%、20%、30%乙醇组,每组5只,乙醇组给予相应浓度乙醇饮用至相应时间;取各组小鼠脑组织,分别采用免疫组化和Western blot方法检测脑皮质神经细胞IP3R1表达的变化;SPSS 13.0软件对实验数据进行单因素方差分析。结果正常IP3R1免疫组化染色物分布于神经细胞胞浆内。90d组随乙醇浓度的增加,IP3R1免疫组化阳性细胞数和阳性细胞率逐步增加,组间比较,差异显著(P〈0.05);180d组中10%、20%乙醇组阳性细胞数和阳性细胞率逐步增加,组间比较,差异显著(P〈0.05),而30%乙醇组阳性细胞数和阳性细胞率反而减少,且低于90d组的相同浓度组(P〈0.05)。Western blot与免疫组化检测结果基本一致。结论慢性乙醇中毒可引起小鼠大脑皮质神经细胞IP3R1表达增加,而高浓度(30%)、长时间(180d)乙醇使IP3R1表达降低,可能与神经细胞变性、坏死、数目减少有关。 相似文献