猪圆环病毒2型套式PCR检测方法的建立及应用

根据GenBank登录的猪圆环病毒 2型 (PCV2 )的全基因组序列 ,设计了 2对引物 ,建立了检测PCV2的套式PCR方法。对该方法用序列分析、酶切等方法进行了鉴定。同时用该套式PCR方法对华南地区 2 87个猪场的 15 6 0份样品进行了检测 ,结果 ,983份为PCV2阳性 ,阳性率为6 3%。     

节约型政府建设的路径分析

本文先从廉洁型政府、务实型政府、生态型政府这个三视角对节约型政府的本质进行阐述;然后进一步对建设节约型政府的必要性进行分析,最后给出了建设节约型政府的若干路径。     

论海运承运人单位赔偿责任限制所涵盖的损失类型——兼对UNCITRAL《运输法》（草案）的评论

在国际海上货物运输中,单位赔偿责任限制是承运人赔偿责任的一项重要内容。结合有关国际公约,重点分析了单位责任限制应当包含的损失类型,同时对中国《海商法》及《运输法》(草案)的相关规定进行了评论并提出修改建议。     

论公务员精神及其培育

公务员精神是其政治素质、思想作风、职业素养、道德情操、精神风貌的集中反映,可分为无私奉献、为民服务、实事求是、团结进取、开拓创新和艰苦奋斗六类精神。当前,培育和弘扬公务员精神既重要又必要,必须以“用高尚的精神塑造人”为指导思想,以“八荣八耻”为原则和要求,从管理、文化、法律三大途径培育和弘扬公务员精神。     

抗人N单克隆抗体的理化特性研究及其初步应用

本文报告理化因素对抗人N单克隆抗体(ON_1D_3)活性的影响。P~H及反应温度对ON_1D_3单克隆抗体与人红细胞的血凝反应有显著的影响。较适宜的实验条件为P~H7.0,温度30℃。在此条件下对155例新鲜血液及60例陈旧血痕样品进行MN分型。结果与用市售兔抗N血清测定的结果完全一致,证明在上述条件下,ON_1D_3单克隆抗体可用于临床常规血液分型及法医学上对陈旧血痕MN血型的测定。     

Influence of substrate tissue type on larval growth in Calliphora augur and Lucilia cuprina (Diptera: Calliphoridae)

The size of fly larvae is an important variable in the use of these insects to estimate postmortem interval. Furthermore, the nutritional intake of larvae is likely to vary subject to the part of a corpse on which they are feeding. A study was therefore conducted to investigate the effect of type of food substrate on larval growth in two species of forensically important Australian blowflies. After collection on sheep's liver in the laboratory, different groups of larvae of Lucilia cuprina (Wiedemann) and Calliphora augur (Fabricius) were grown on sheep's liver, meat, and brains, and their body lengths compared. Results indicated that the development of larvae fed sheep's liver was adversely affected compared with larvae fed meat and brain; they moulted later, reached maximum length more slowly and sometimes produced significantly smaller pupae. These findings, similar to those of another recent study, have obvious implications for postmortem interval determinations. Estimates may be considerably skewed if the site of collection of larvae at a death scene contains tissue types different to those used in reference experiments. We therefore recommend caution in forensic analyses that interpret crime scene data using developmental studies performed with a single type of larval food substrate.     

知柏地黄口服液及丸的药效学和急性毒性

Both forms in koufuye and wan could increase the hypoxia tolerance of mice with hyperthyroidism of Yin deficient type, recover both the decreased body-weight and the weight index of adrenal gland of rats and the increased creatinine with urea nitrogen in plasma which were Yin deficient trpe of hydracortisone. They could also increase the phagocytic percentage of macrophage and neutrophilic granulocyte. The LD50 has not been measured even ig with maximal concentration and maximal volum of the drugs for 4 times in 24 hours.     

浅谈高等职业教育的人才培养

大力发展高等职业教育是经济和社会发展的客观需要,是贯彻党的“教育要全面适应社会主义现代化对各类人才的需求,提高教育质量和办学效益”的方针。本文论述了高等职业教育的培养目标及其人才类型的特点,明确了高职人才质量构成的要素,对高等职业教育进行了理论探讨,提出了新的人才培养观念。     

浅析明代"家乐"问题

明代兴起的"家乐"与前代在勾栏瓦舍中戏班的演出有所不同,一方面这种形式为传统戏剧的改良提供了硬件的支持,另一方面它也有自身不可克服的弱点.基于自身优势,家乐在明中叶后盛极一时,但同时又因为先天缺陷,在清中叶就几近销声匿迹.     

胃脘痛证候的聚类分析及因子分析研究

目的 采用聚类分析及因子分析的数据挖掘方法,对胃脘痛的中医临床证候及四诊信息进行研究。方法 收集640例755诊次胃脘痛病例,记录症状、舌象和脉象等临床资料,对临床资料进行聚类分析及因子分析。结果 胃脘痛临床证候可分为六类:脾胃虚寒、肝气犯胃、湿热中阻、脾胃气虚、痰湿内蕴、瘀血停胃,每一类证候对应贡献率不同的特异的四诊信息。结论 胃脘痛有特异的证候分类及四诊信息,通过数据挖掘方法深入研究,对胃脘痛的规范化诊断提供了有效的数据支持,能提高辨证准确性。