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排序方式: 共有126条查询结果,搜索用时 328 毫秒
1.
利用RT-PCR方法扩增了猪ERK6基因编码区的序列,将扩增产物与pMD18-T载体连接,构建了重组质粒;重组质粒经PCR、酶切鉴定后进行测序;采用Northern杂交和半定量RT-PCR方法在猪不同部位骨骼肌中分析了ERK6基因转录本的个数、大小及组织表达谱。结果显示,所克隆的猪ERK6基因片段长1 113 bp,含有1个1 104 bp的开放阅读框(ORF),该ORF编码367个氨基酸;该基因与已报道的人ERK6基因核苷酸序列相似性为90%;猪ERK6基因在肌肉组织中只有一个转录本,大小约1.5 kb。此基因在骨骼肌中的表达量最高,心、子宫次之,卵巢最低;而在脂肪、胃、肝、脾、肺、肾、十二指肠和胰腺中未见表达。结果表明,猪ERK6基因与已报道的小鼠和人ERK6基因一样,主要在骨骼中特异表达。 相似文献
2.
Updegraff Kimberly A. Helms Heather M. McHale Susan M. Crouter Ann C. Thayer Shawna M. Sales Lara H. 《Journal of youth and adolescence》2004,33(5):403-420
This study examined the nature and correlates of different patterns of perceived control in adolescents' relationships with their best friends. Participants included firstborn adolescents (M = 14.94 years), their younger siblings (M = 12.44 years) and both their mothers and fathers in 163 families as well as a best friend of each adolescent (M = 15 years). Data were collected from family members during home visits regarding adolescents' family relationships, friendships, and psychosocial adjustment; time use data were gathered during a series of 7 nightly phone interviews. Information was obtained from best friends during a brief phone interview. We developed a typology of 3 different patterns of perceived friendship control based on the combination of adolescents' and their best friends' ratings of relational control. Patterns of control in adolescents' friendships were associated with the distribution of control in both parents' marriages and adolescents' sibling relationships. Further analyses, designed to test developmental predictions, revealed connections between friendship control and other qualities of adolescents' friendships (i.e., intimacy, conflict, perspective-taking). 相似文献
3.
Rosario Barranco M.D. Fiorella Caputo M.D. Davide Bedocchi M.D. Francesca Maria Elena Frigiolini M.D. Lara Castelletti M.D. Giulio Fraternali Orcioni M.D. Francesco Ventura M.D. Ph.D. 《Journal of forensic sciences》2020,65(1):314-317
Intravascular lymphoma (IVL) is a rare subtype of extranodal lymphomas that is characterized by the selective growth of neoplastic cells within the lumen of small vessels. Authors document the case of an unexpected death caused by an undiagnosed intravascular large B-cell lymphoma with multi-organ involvement, which had initially manifested as an infection and then as an unclarified central nervous system pathology. Histological examination showed a diffuse intravascular large B-cell brain lymphoma with prominent cerebral involvement. The relevance of the case report reveals the importance of an autopsy of an extremely rare and threatening pathology that in most cases is diagnosed only postmortem. As a result, the role of the forensic pathologist becomes particularly important. When specifically performing an in-depth autopsy evaluation with a specific histologic analysis, it is possible to identify the intravascular lymphoma and declare a more accurate cause of death. 相似文献
4.
BA Shi 《今日中国(英文版)》2006,55(3):46-47
YANG Jie, 23, starts work at his office on Youth Road in Wuhan, Hubei Province at 9 am each day and returns to his dorm to sleep at 10 pm. He eats a modest lunch in the small restaurant downstairs and has a takeaway for supper. It was in the summer of 2005 that Yang Jie and Li Chao opened their Qi Hang Advertising Company. Their small firm deals with large and small concerns alike, and in order to stay solvent, they spend their lives either at the office, where they deal with clients an… 相似文献
5.
6.
Calacal GC Delfin FC Tan MM Roewer L Magtanong DL Lara MC Fortun Rd De Ungria MC 《The American journal of forensic medicine and pathology》2005,26(3):285-291
In a fire tragedy in Manila in December 1998, one of the worst tragic incidents which resulted in the reported death of 23 children, identity could not be established initially resulting in the burial of still unidentified bodies. Underscoring the importance of identifying each of the human remains, the bodies were exhumed 3 months after the tragedy. We describe here our work, which was the first national case handled by local laboratories wherein conventional and molecular-based techniques were successfully applied in forensic identification. The study reports analysis of DNA obtained from skeletal remains exposed to conditions of burning, burial, and exhumation. DNA typing methods using autosomal and Y-chromosomal short tandem repeat (Y-STR) markers reinforced postmortem examinations using conventional identification techniques. The strategy resulted in the identification of 18 out of the 21 human remains analyzed, overcoming challenges encountered due to the absence of established procedures for the recovery of mass disaster remains. There was incomplete antemortem information to match the postmortem data obtained from the remains of 3 female child victims. Two victims were readily identified due to the availability of antemortem tissues. In the absence of this biologic material, parentage testing was performed using reference blood samples collected from parents and relatives. Data on patrilineal lineage based on common Y-STR haplotypes augmented autosomal DNA typing, particularly in deficiency cases. 相似文献
7.
James C. Spilsbury Lara Belliston Dennis Drotar Allyson Drinkard Jeff Kretschmar Rosemary Creeden Daniel J. Flannery Steve Friedman 《Journal of family violence》2007,22(6):487-499
This study assessed the associations of characteristics of domestic violence incidents with clinically significant levels
of traumatic symptoms and behavioral problems in a socio-economically and ethnically mixed sample of 687 children participating
in a community-service program for children witnessing violence. Study predictors included child/family demographic characteristics,
type and chronicity of exposure, and child’s perceptions of control over the event and threat to personal safety. Outcomes
consisted of traumatic symptoms and behavior problems. Results showed that perceived threat and control were associated with
greater odds of clinically significant levels of several trauma symptoms (and behavior problems in the case of perceived threat)
after adjusting for effects of demographic factors and violence characteristics. Child co-victimization increased odds of
reaching clinically significant levels of traumatic symptoms compared to children who witnessed the event but were not victimized.
Female sex and White ethnicity increased odds of specific trauma symptoms and behavior problems. Increasing age reduced odds
of some trauma symptoms. Associations between predictors and one outcome measure did not generalize across the other outcome
measure. Implications of study findings, and directions for future research are discussed. 相似文献
8.
Trilogues have been studied as sites of secluded inter-institutional decision making that gather the Council of the European Union, the European Parliament (EP) and the European Commission. Trilogues, however, are not exempt from formal and informal party-political dynamics that affect intra- and inter-institutional contestation. The increase in Eurosceptics in the 2014 EP elections offers an opportunity to investigate their efforts to shape the position and behaviour of the EP negotiating team in trilogues. Therefore, this article investigates to what extent Eurosceptic party groups participate in trilogue negotiations and how mainstream groups deal with their presence. The analysis shows that the opportunities to participate in trilogues and shape the EP’s position are higher for those perceived as soft Eurosceptic MEPs, while mainstream groups apply a ‘cordon sanitaire’ to those perceived as being part of hard Eurosceptic groups – which reduces the chances of MEPs from those groups being willing to participate in parliamentary work. 相似文献
9.
Casiano Manrique de Lara Peñate Dolores Santos Peñate 《Economic Change and Restructuring》2003,36(3):259-272
Many structural relationships should be taken into account in any reasonable adjustment and updating process. These structural relationships are mainly represented by ratios of different types, such as technical coefficients or the proportion of the cell value in relation to its row or column total. We believe that in many cases (either because of lack of information or when the time elapsed for the estimation of a social accounting matrix is not long enough to allow for any significant structural change) the updating process should try to minimize the rela- tive deviation of the new coefficients from the initial ones in a homogeneous way. This homogeneity would mean that the magnitude of this relative deviation is similar among the elements of each row or column, therefore avoiding the concentration of the changes in particular cells of the SAM. In this work, we propose some new adjustment criteria in order to obtain a more homogeneous relative adjustment of the structural coefficients. These criteria combine the adjustment method proposed by Matuszewski et al. (1964) with other deviation functions. Each of the adjustment criteria proposed leads to a nonlinear optimization problem which is reformulated as a linear program. We test the usefulness of this proposal by comparing its results with the ones obtained by more standard approaches and we are able to show that these approaches tend to produce a less homogeneous pattern of coefficient adjustment, under certain circumstances, than the ones we put forward. 相似文献
10.
进入20世纪80年代以后,作为经济强国的日本已经不再满足于“经济大国政治小国”的地位,历届内阁开始要求与经济大国地位相适应的政治大国地位。日本政府在寻求实现政治大国目标的过程中,主要选择了以军事领域作为突破口,以成为军事大国带动政治大国目标的实现。具体体现在强化日美同盟、建立和完善有事法制、保持自主、高效的自卫力量与积极参与联合国维和行动四个方面。 相似文献