双峰驼诱导排卵因子的生物学活性测定

公驼精清经ＤＥＡＥ－纤维素离子交换层析，共收集到７个蛋白组份，回归本动物，确定了可以诱导母驼排卵的组份，并经等电聚焦电泳加以证实，此外，分析了公驼精清的氨基酸组成，并对活性组份试行进一步纯化。     

双峰驼诱导排卵因子氨基酸组成的分析

分别测定了纯化的公驼精清诱导排卵因子７的活性和固定部分肽类的小肽氨基酸组成,证明活性位序易降解,这一特性与其所含的丝氨酸、谷氨酸、甘氨酸等有关。     

双峰驼诱导排卵因子活性肽的分离

从双峰驼精清内分离出一种新的活性多肽。这种多肽是将公驼的精清经ＤＥＡＥ—纤维素和ＳｅｐｈａｃｒｙｌＳ—２００柱层析加以初步分离。在试验动物上测试，找到可以诱发母兔排卵的活性多肽。用十二烷基硫酸钠—聚丙烯酰胺凝胶电泳（ＳＤＳ—ＰＡＧＥ）和等电聚焦电泳（ＩＥＦ）测定了这种多肽的分子量和等电点。     

单切口双结扎缝一针公驼去势法

单切口双结扎缝一针公驼去势法公驼去势多沿用双切口不缝合的夹板骟及烧烙骟，其创口易感染、愈合慢，术后出血及阴囊内容物脱出常有发生。为克服上述缺点，笔者采用单切口、双结扎、缝一针的方法对１１８峰公驼去势，均收到较好效果。手术方法驼左侧卧保定，术部常规消毒...     

双峰驼诱导排卵因子活性位序列测定与肽链结构研究

应用高效液相色谱仪、蛋白顺序仪和傅里叶变换离子回旋共振质谱仪等仪器 ,纯化并测试了公驼诱导排卵因子(OIF)的部分活性序列和降解过程。结果表明 ,公驼OIF是单链多肽 ,分为活性序列和相对稳定固定序列两部分 ,N 端的活性位序仅为 6个残基 :赖氨酸 缬氨酸 丙氨酸 苯丙氨酸 丝氨酸 苏氨酸。该因子的氧化降解过程在冰冻条件下是由氨基酸侧链基团的氧化和肽链的断裂共同引发的。     

新疆驼的心电图

材料和方法 (一)试验条件 1.随机选择临床健康的成年新疆驼(双峰驼)39峰,其中母驼14峰、公驼3峰、去势驼22峰,年龄6～26岁。试验当时的体温平均37.5℃(37.1～38.4℃)。驼群放牧,试验前由放牧地赶来,休息约2小时后开始测试。 2.试验驼在四柱栏内保定,栏内地面垫以3～4厘米厚的木板,测试时骆驼自然站立在此木板上,四肢不互相接触,身体任何部分亦不与栏柱接触,以保证良好的绝缘状态。 3.使用国产ZXD—1型热笔式心电图机,普通照明电源,妥善地接好地线(在室外湿地上埋入约50厘米长的细铁棒),严格按照该型心电图机的使用规程进行操作。     

双峰驼精清蛋白图谱及分子量的测定

采用ＳＤＳ－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）观察了双峰驼精清及其对照组牛、羊、猪精清电泳蛋白图谱，测定了各蛋白组份的分子量。结果表明，双峰驼精清在分子量７２３９０～１２８８１Ｄ间形成１７条蛋白区带，在７２３９０～４３１７７Ｄ间，与牛、羊、猪精清蛋白图谱相似；但在较小分子量中，在分子量为４０６８５，２０８４４及１５６９３．３Ｄ的三条蛋白区带处，有与牛精清蛋白位置上相近的蛋白区带，但含量及分子量尚有差异。     

双峰驼胰腺炎的临床病理学研究

对长沙动物园２ 峰病死驼进行了临床病理学研究，根据死驼的临床表现、病理剖解及组织学变化，结合实验室酶学检测，推断２ 峰双峰驼死于急性胰腺炎     

国产盐酸二甲苯胺噻唑对骆驼的药效观察

(一)材料与方法 1.试验动物:双峰驼21峰,其中母驼8峰,骟驼13峰,年龄12～20岁,膘份中上等,体重386～650公斤。全部试验驼意识清楚,反应正常,肌肉紧张有力,健康无病。 2.药品:2％的2.4～二甲苯胺噻唑,丹东兽药厂试产。 3.试验方法: (1)给药途径:肌肉注射。     

双峰驼初乳IgG的分离纯化及其向幼驼的转移

通过跟踪检测内蒙古阿拉善双峰母驼初乳IgG和幼驼血清IgG的动态含量,探讨了影响幼驼由母体获得被动免疫的因素。采用硫酸铵沉淀法2次沉淀乳清蛋白,再结合离子交换层析方法,从双峰驼初乳中分离纯化驼IgG;再用SDS-PAGE鉴定其纯度,测定其分子质量;用分离纯化的驼IgG免疫家兔,制备效价至少为1∶32的兔抗驼IgG抗血清,采用单向免疫扩散法测定双峰驼初乳和幼驼血清IgG含量。揭示了初乳IgG向幼驼血液的转移模式,为生产中更好地饲养幼驼提供科学依据。     

羊驼副性腺结构的组织化学研究

为了解羊驼的生殖生理特性,采用大体解剖与组织学方法对羊驼副性腺的形态结构进行了研究。结果显示,羊驼副性腺有前列腺和尿道球腺,没有精囊腺。尿道球腺呈圆形,位于坐骨弓处,前列腺位于尿生殖道起始部的背侧,每一腺体都有腺管开口于尿生殖道;羊驼副性腺的基本组织结构和其他哺乳动物相似,但有较小的差异。尿道球腺为有许多弯曲分枝的复管泡状腺,分泌物较为黏稠,PAS染色强阳性,呈鲜红色;前列腺为复管泡状腺,前列腺的分泌物是一种稀薄的蛋白样液,PAS染色阳性,染色较浅。结果证实,羊驼前列腺和尿道球腺可分泌较多的糖物质,使精子生存较长时间;羊驼繁殖慢的特点可能与其生存环境差,易造成流产或妊娠期长且为单胎有关,而并不是精液本身的问题。     

东方蝾螈的形态观察和解剖学研究

采用解剖学方法对8尾东方蝾螈的外形和内脏进行了系统研究。结果表明,东方蝾螈的口裂很宽,口咽腔底壁的前方有不发达的舌。食管短,黏膜上有纵行皱襞;胃呈纺锤形的长囊,肌层发达,贲门口较大;直肠粗大;肝较为发达,有胆囊;胰独立,不与肝混合,但胰管与胆管合并。肺为2个锥形的薄壁长囊,构造简单,囊壁形成蜂窝状。输尿管不直接通膀胱,尿液先到泄殖腔,然后再到膀胱。雄性大鲵无专门的输精管,而是借助于输尿管输精。雌性大鲵有卵巢1对,呈带形的囊状卵巢。     

列当的安全性评价试验

为进一步确定列当(Orobanche cumana)的实际效用以及是否可以在成为肉苁蓉的替代品方面有其相应的价值,对小鼠进行半数致死量测定和最大给药量测定的急性毒性试验、SD大鼠进行30 d灌胃的亚慢性毒性试验,观察列当水煎液的毒性作用。急性毒性试验结果表明,当列当水煎液的最大给药剂量为40 g/kg时,小鼠并无不适反应,体征一切正常,7 d内所有小鼠无死亡。亚慢性毒性试验期间,试验组大鼠的临床体征正常,血常规及血清生化指标均在正常范围内,剖检未见异常,在心、肝、肾组织学观察中均未见明显的病理变化。列当水煎液急性毒性试验及亚慢性毒性试验均说明列当水煎液实际无毒。     

The role of communication in managing the safety climate of construction site environments

Managers in the construction sector are met with a critical charge: they are responsible for the safety of employees in one of the most notoriously dangerous industries in the world. In terms of managing the safety climate of construction site environments, no recommendations have been made in the literature that truly elucidate the role of organisational communication therein. The aim of this research was to fill this void by enriching the seminal work of Mohamed (2002) which focusses on the nature of the safety climate in construction organisations, and the factors it is comprised of. To this end, the research comprised a data-triangulated qualitative and quantitative empirical study undertaken at nine different construction sites in South Africa, which allowed for the reformulation of the model for safety climate management in construction environments, with an added understanding of the role of communication therein. The findings indicated that communication is conducive to a positive safety climate when it is managed to be strategic, holistic, relational and symmetrical. The model put forward in this article offers an empirical application of the four identified constructs of communication, which gives way to data-driven recommendations for use in construction organisation settings.     

Jihadism as a Subcultural Response to Social Strain: Extending Marc Sageman's “Bunch of Guys” Thesis

My aim in this article is to extend Marc Sageman's seminal research on Al Qaeda by re-articulating it through the prism of Albert Cohen's theory of delinquent subcultures, an approach which came to prominence in Criminology in the 1960s, but has since then been largely eclipsed by other approaches in that field. Drawing on Sageman's findings and observations, I suggest that Al Qaeda-affiliated or -inspired groups in the West can be best understood as a collective response or “solution” to the strains encountered by the members of these groups, and that these strains are imposed on them by the circumstances in which they find themselves. My broader aim is to show that although Criminology, with a few exceptions, ignores the subject of terrorism, terrorism studies can appreciably benefit from an engagement with Criminology as a source of theoretical inspiration.     

乳牛产后血浆瘦素动态与亚临床酮病的相关分析

对某奶牛场16头乳牛产前10 d至产后56 d血样的瘦素、酮体、葡萄糖、脂肪水平及其动态特征和相关性进行了检测。结果表明,血浆瘦素、血酮、血脂含量在不同乳牛和不同时间点之间均有显著差异。酮体在产后14~49 d出现高峰,瘦素和血脂从产后第0 d到产后第56 d内逐渐升高。瘦素水平与血脂水平呈极显著正相关,血酮与血糖呈极显著负相关。亚临床酮病组乳牛的瘦素和血脂水平分别极显著或显著低于血酮正常组,其瘦素、血酮和血糖在试验期内的波动频率明显减少,跨度变长,瘦素在试验期内仅出现一个两端接近0、波峰不超过1.5 ng/mL的波。证实,产后乳牛瘦素、血酮、血糖含量的低频率波动变化和产后8周内血浆瘦素跨时持久的单峰动态变化与乳牛亚临床酮病的发生有关。     

Multiculturalism,recognition and the “Kurdish question” in Turkey: the outline of a normative framework

Despite the ever-growing literature on various aspects of the Kurdish question in Turkey, there are practically no normative discussions of Kurdish demands for the public recognition of their identities. This is all the more startling given the ascendancy of philosophical accounts in the literature on minority rights. This article will try to redress the balance by proposing a normative framework that could be used to justify Kurdish demands for recognition, drawing on the broader debates that revolve around Charles Taylor's seminal conceptualization of “the politics of recognition”. In this context, I will identify nationalism, a strategy used both by the state to suppress demands for recognition and by the minorities to put forward these demands, as the most serious hurdle in the way of the democratic resolution of the “Kurdish question” in Turkey and promote a form of recognition-based multiculturalism that (a) respects the basic rights of all citizens, not only those in whose name the said claims are made, but also those of the members of the majority against which the claims are voiced; (b) leaves room for alternative values or conceptions of the good life.     

Another Weber: state,associations and domination in international relations

While there has arguably been a partial reception of Weber in international relations (IR), we argue here that his ideas have either been misunderstood or neglected. In order to highlight the most valuable Weberian insights, we focus on two topics of crucial importance to IR. First, in our view, Weber's crucial contribution to the study of states is not his alleged emphasis on the monopoly of violence but his concern with the problem of legitimacy, which is the key to understanding why individuals actually orient an action according to their beliefs in the idea of a state. Second, Weber conducted seminal historical investigations on religion, the rationalization of economic ethics, and organizations that show that the diffusion of isomorphism has little to do with supposedly uninterested and persuasive scientific and professional associations. Instead, more attention should be paid to rational domination and less to reified concepts such as John Meyer's ‘rationalized otherhood’. These arguments are also examined empirically.     

用ELISA和PCR检测异常妊娠妇女弓形虫感染

为了解兰州市妊娠妇女近年弓形虫感染情况 ,探讨异常妊娠与弓形虫感染的关系 ,分别采用ELISA和PCR检测了异常妊娠妇女血清中TOX IgM和TOX DNA。结果 ,1 3 5例异常妊娠妇女血清TOX IgM和TOX DNA阳性率分别为 1 1 .1 1 % (1 5 /1 3 5 )和 1 2 .5 9% (1 7/1 3 5 ) ,总阳性率为 1 3 .3 3 % (1 8/1 3 5 ) ,组间无显著差异 (P >0 .0 5 )。异常妊娠史妇女与妊娠期无异常孕妇的血清TOX IgM阳性率分别为 1 1 .1 1 % (1 5 /1 3 5 )和 6.0 2 % (3 2 /5 3 2 ) ,组间差异显著 (P <0 .0 5 ) ,相对危险度为1 .85。调查结果显示 ,兰州市弓形虫血清抗体阳性率较 1 994年略有上升 ,弓形虫感染的孕前检查应联合应用ELISA和PCR为宜。     

Measuring and comparing party ideology in nonindustrialized societies: taking party manifesto research to Africa

Despite a growing interest in African parties, no comparative analyses of African party manifestos have been undertaken to date. This study applies the Manifesto Research Group's (MRG/CMP) coding scheme to a complete set of manifestos in three countries. The study's main aim is to determine whether a research tool that has been seminal in the study of Western politics can be used to advance the study of political parties in nonindustrialized societies. In a first step, the article examines the extent to which African manifestos advance programmatic ideas. The results show great differences across parties and time. The study subsequently investigates how parties position themselves on a right–left spectrum; it further outlines which policy categories African parties stress most. Finally, it examines the stance of individual parties on specific policy issues. The study argues that the MRG coding scheme can contribute to a much more nuanced analysis of African parties.