精斑中磷酸葡萄糖变位酶及其亚型的电泳分型

<正> 本文用淀粉凝胶电泳法和PAGIEF对精斑PGM_1普通型及亚型进行了检测。对169例不同个体精斑PGM_1分型,三个普通型的出现频率PGM_1 1—1 87;PGM_1 2—1 66;PGM_1 2—2 16。对31例同一个体红细胞及精斑PGM_1分型,其结果完全一致。对138例不同精子     

超薄层等电聚焦电泳检测红细胞PGM_1亚型

我们采用超薄层聚丙烯酰胺凝胶等电聚焦(UTL PAGIEF)电泳技术检测了红细胞PGM_1亚型,并对郑州地区356例汉族无关健康个体进行了PGM_1亚型分析。现将结果报告如下: 表1为郑州地区356例无关健康个体的PGM_1亚型分布。按Hardy-Weinberg平衡定律进行吻合度检验,x~2=4.3154;df=6;0.50相似文献   

中国人(辽宁地区)PGM_1亚型的分布及一例PGM_1china罕见型的报导

1964年Spencer, Hopkinson及Harris在用淀粉凝胶电泳对人类红细胞内酶类的研究中首次检测出由PGM_11位点上的两个常见等位基因(PGM_11和PGM_12)所决定的3个常见表现型:PGM_1~1、PGM_1~2及PGM_12—1。后来,不少学者对PGM_1进,行了进一步的研究,相继发现了一些罕见等位     

精浆r—谷氨酰转移酶多态性研究

本次实验采用聚丙烯酰胺阶段梯度凝胶电泳方法,对武汉地区135名无关男性精液γ—GT进行了遗传多态性调查,根据γ—GT的电泳差异,分为1型、2型、2—1型三种常见表现型,1型为靠近正极的单一带,2型为靠近负极的单一带,2—1型为1型带和2型带组成的杂合双带。表现型频率分别为0.156,0.356,0.488,基因频率为γ—GT~1=0.400,γ—GT~2=0.600(P>0.5)。室温下保存8周的精斑,也能检测出较清晰的谱带。本次实验还探讨了单纯阴道分泌物及精液、阴道分泌物混合斑进行γ—GT分型的可能性。     

孕早期绒毛膜PGM_1多态性研究

我们采用混合淀粉凝胶电泳和聚丙烯酰胺凝胶等电聚焦电泳的方法,分析了妊娠早期绒毛细胞及父母红细胞的葡萄糖磷酸变位酶-1(PGM_1)的多态性。结果证明妊娠早期绒毛中可查出 PGM_1酶型,按孟德尔法则遗传。共观察到3种常见遗传表型和10种常见亚型。     

应用等电聚焦电泳对人精液黄递酶Ⅲ表型分布的研究

应用超薄层聚丙烯酰胺凝胶等电聚焦电泳对武汉地区198例成人精液黄递酶Ⅲ(DIA_3)作了分型。观察到4种表现型,基因频率分别为DIA_3~1=0.7727;DIA_3~2=0.2172;DIA_3~3=0.0101。对保存精斑进行检测证实,室温下保存9周的精斑仍能作DIA_3分型。在聚丙烯酰胺凝胶中引入分离剂HEPES,提高了分辨率。改进后的等电聚焦技术尤适于DIA_3基因产物的分离。文中还调查了我国汉族人群DIA_3频率分布,并与其它群体资料作了比较。     

用同步电泳法检测内蒙地区纯蒙古族人群ESD、GLOI、PGM_1表型分布及基因频率

<正> 作者采用淀粉琼脂糖混合凝胶电泳法,在一块凝胶板上同时分析血液或血痕的ESD、GLOI、PGM_1三种红细胞酶型,并调查了内蒙地区254名无血缘关系的纯蒙古族健康献血员的三种红细胞酶型的表型分布及基因频率。检出ESD、GLOI、PGM_1均有1-1,2-1,2-2三种常见表型,根据表型频率计算出这三种酶型在内     

用ELISA检测精液及精斑中精子抗原的研究

本实验应用单克隆抗人精子抗体和酶标记羊抗人精子抗体,采用ELISA方法确定精子抗原成份的存在。对10份新鲜精液,15份精斑进行了验测,其结果阳性率为100%。新鲜精液(精子数约10,000万个/ml)稀释100万倍,精斑浸出液稀释50万倍,均可出现阳性。对唾液斑、尿斑、乳汁斑、阴道斑、汗斑及输精管结扎的精液均为阴性。实验结果表明,本法检验精子抗原具有灵敏度高,特异性好的优点。     

血痕中PGM_1亚型的检出及酶谱的保存方法

本文报告用等电聚焦方法,采用拉丁方设计,对血痕保存的温度、布质及含量,以及血痕中 PGM_1亚型检出时间进行了研究。保存在0℃(6个月)、4℃(2个月)、18℃(1个月)及30℃(3周)的6μL 血痕,PGM_1亚型均可检出。血痕的总量对 PGM_1亚型的检出时间也有一定的影响。不同布质对血痕中 PGM_1亚型的检出时间,无明显差异。另外,利用聚脂膜具有亲水膜面的特点,将 PGM_1原始酶谱贴附在聚酯膜上,可长期保存酶谱。     

阴道液与血液中PGM_1亚型、EsD、GLOI表型相关性的研究

本文用等电聚焦电泳法和琼脂糖淀粉混合凝胶电泳法,对阴道液、血液中的PGM_1亚型、EsD、GLOI表型进行了检测。在部分妇女的阴道液中未检出其本人血液中的这些酶型,测出的比例最高的是PGM_1亚型,EsD次之,GLOI最低。凡能显示清晰酶谱带的均与同一妇女血液中的表现型相同,未见不一致的现象。同时初步探讨了月经周期和性刺激对阴道液中PGM_1酶活性的影响。     

红细胞EsD和PGM_1的同步电泳分型及其在上海地区的分布与频率

用 pH7.4的 Tris-马来酸缓冲系统和混合淀粉凝胶同步检测血液及血癌中 EsD 和 PGM_1的表型,获得良好的分型效果。EsD 和 PGM_1的图谱区带平直、狭窄、清晰。各种表型之间差异著,极易区分容易发现稀有表型。我们在上海地区居民中检查了390人的 EsD 表型和724人的 PGM_1表型,其分布与其基因频率详见附表。在检测尸体血及尸体血痕时,发现一例尸体血和一例尸体血痕的 PGM 1活性明显增强,前者尚显现了一条额外的同工酶区带。     

Simultaneous identification of alpha-L-fucosidase and phosphoglucomutase (PGM) subtyping in semen stains

Seminal fluid and stains were analyzed by isoelectric focusing to determine the donor phenotype in the alpha-L-fucosidase (AlFuc) polymorphic system. The enzyme is found in both seminal fluid and spermatazoa. Three common phenotypes exist and can be identified in fluid specimens stored at 4 degrees C for more than a year. Untreated semen specimens display more than eight distinct bands of alpha-L-fucosidase activity with isoelectric points of pH 6.6 and below. Neuraminidase-treated specimens have enhanced banding patterns cathodally with a loss of activity in anodal bands making it easier to phenotype specimens. Semen stains maintained in dehumidified chambers at 25 or 37 degrees C retained activity for at least one month and could be accurately phenotyped. Activity was observed in semen specimens maintained at -20 degrees C in the dried state for a period of one year, whereas a complete loss of activity was observed after two weeks in similar specimens maintained at 25 or 37 degrees C under humid conditions. Of seventy-four semen stains analyzed, two had no apparent activity. Of the remaining seventy-two specimens 56, 32, and 12% were phenotyped as FUC 1-1, FUC 2-1, and FUC 2-2, respectively. Calculated gene frequencies are FUC1 = 0.72 and FUC2 = 0.28. Following analysis of alpha-L-fucosidase, the agarose gel can be chemically developed to reveal the PGM1 subtyping pattern. The ability to phenotype both systems in semen stains significantly improves the ability of the analyst to individualize this type of physical evidence. The probability of discrimination for these two combined systems is approximately 0.89.     

DIA3 phenotyping in human semen and seminal stains by isoelectric focusing

The polymorphism of DIA3 was investigated by isoelectric focusing in semen samples from 235 unrelated Japanese volunteers and patients. Besides the three common phenotypes seven samples of the type 3-1 were observed. However, readable isoenzyme patterns were not demonstrated in semen samples of oligospermia under about 10 X 10(6)/ml sperm cells. The allele frequencies were DIA3*1 = 0.821, DIA3*2 = 0.164, and DIA3*3 = 0.015. The DIA3*1 frequency in oligospermia (0.765) was lower than that in normospermia (0.836). The isoelectric focusing method was successfully applied to phenotyping DIA3 in seminal stains; each phenotype was demonstrated at 37 degrees C for up to 4 weeks, at room temperature for up to 8 weeks, and at 4 degrees C for over 12 weeks after stain formation. In vaginal swabs the isoenzyme bands were very faint and not identifiable.     

Detection of orosomucoid 1 phenotypes in semen and semen stains

Orosomucoid 1 phenotypes were detected in seminal plasma by isoelectric focusing and immunoprinting. The orosomucoid 1 phenotypes in seminal plasma correlated with the types found in the corresponding serum specimens. Semen stains stored for ten days could be typed for orosomucoid 1. The present work revealed that orosomucoid 1 is a useful genetic marker for the medicolegal grouping of semen stains.     

用等电聚焦酶免疫分析法检测人类精斑GC表现型

本文用IEF结合使用过氧化物酶标记第二抗体的酶免疫分析法检测了17名键康成年男性的精液(斑)和唾液斑及10名健康成年女性的阴道分泌液中的GC表现型。结果发现17份人类精斑均可测出三种GC蛋白普通表现型。在10份阴道液中测出一份样本的GC表现型,3份样本有不甚清楚的GC带,不能定型,其他样本均无GC带。17份唾液斑未测出GC。本法的灵敏度(0.675ng)比文献报道的用过氧化物酶标记第二抗体的酶免疫分析(5.6ng)高。     

Studies on glyoxalase 1 isoenzymes in semen stains: polymorphism in Himachal (India) population

One hundred and ten pairs of blood and semen samples and their stains were studied to type glyoxalase 1 (GLO 1) isoenzymes using agarose-starch medium. A good agreement was observed between the phenotypes expressed in blood and semen samples of the same donor. No GLO 1 activity however could be demonstrated in the vaginal swabs tested. The gene frequencies of GLO 1 polymorphs in Himachal population has been worked out and their stability studies carried out at -12 degrees C and at room temperature.     

中国人精子黄递酶Ⅲ型的分布及精斑黄递酶Ⅲ型的检出

应用超薄层聚丙烯酰胺凝胶等电聚焦技术对辽宁地区200例正常人精子黄递酶Ⅲ型(DIA)的分布进行了研究。其基因频率分别为DIA_3~1=0.6850,DIA_3~2=0.2875,DIA_5~3=0.0275。由阴道液、唾液、乳汁及无精子症精液不能判定DIA_3型。室温保存精斑可检出时限为9个月。     

辽宁汉族人血清类粘蛋白(ORM)型的分布及其在血痕精液(斑)及脑脊液中的检出

本文作者采用PAGIEF结合免疫固定法,调查了辽宁汉族群体(431人)ORM的分布,检出了ORM1型和ORM2型各8种表现型;对不同条件下的血痕标本进行检测,成功地检出了37℃保存2年血痕的ORM1型;首次由脑脊髓液中检出了ORM1型。ORM型的总鉴别机率为0.7043,是进行个人识别和亲子鉴定的良好遗传标记。     

Determination of C3 phenotype in dried bloodstains using isoelectric focusing

Fresh whole blood and bloodstains were analyzed by isoelectric focusing (IEF) to determine the C3 phenotype of the blood donor. Three common phenotypes exist as a result of two autosomal alleles. The three phenotypes can be identified in fresh serum or in serum samples which had been stored at -20 degrees C for more than a year. Bloodstains maintained in a desiccator at 25 or at 37 degrees C retained the native form of C3 which could be detected for at least two weeks. Beyond two weeks of storage, stains became difficult to phenotype due to decreased banding intensity. Bloodstains aged longer than one month could not be phenotyped. C3 could not be detected in human semen by the serological methods employed.