The Stability of Prostate-Specific Antigen in Semen Under Various Temperatures

Prostate-specific antigen (PSA) is most commonly used for identifying semen, especially in the absence of sperm. However, PSA concentration varies according to storage temperature and duration, and little is known about its stability in semen. This study was therefore aimed to determine the stability under five different temperatures: −80, −20, 4, 25, and 37°C; and nine different durations: 1, 2, 3, 5, 7, 14, 30, 90, and 180 days. All samples were stored at −80°C after being secreted from the volunteers' body until analyzed. Results showed that the PSA concentration declined significantly over time under all temperatures studied except −80°C. At −20 and 4°C, PSA was still detectable on Day 180 with 50% and 70% decrease from its original concentration, respectively. At 25 and 37°C, PSA was detected up to Day 7 and 3, respectively. This information might assist forensic scientists understand more about PSA nature and integrate it into their works.     

An Unusual Case of Death Probably Triggered by the Association of Buprenorphine at Therapeutic Dose with Ethanol and Benzodiazepines and with Very Low Norbuprenorphine Level

Buprenorphine is largely prescribed for maintenance treatment in opioid dependence due to its safety profile. Nevertheless, fatalities at therapeutic dose have been described when associated with other central nervous system depressants, such as ethanol or benzodiazepines. Here, we report a case of death due to association of buprenorphine at therapeutic dose with benzodiazepines and ethanol. Although toxicity has been often attributed to its metabolite norbuprenorphine rather than to buprenorphine itself, in our case, norbuprenorphine was not detected in urine and bile and only in traces in blood. Moreover, the presence in blood of free buprenorphine but not of glucuronide metabolites argues for an unusual early death, at the beginning of buprenorphine metabolism. We propose that in the context of prior toxic impregnation, buprenorphine directly (and not via its metabolite norbuprenorphine) acted as a triggering factor by blocking the ventilatory response, rapidly leading to fatal respiratory depression.     

己烯雌酚人工抗原的合成研究

采用三步反应合成了己烯雌酚人工抗原 ,为己烯雌酚酶免疫检测技术的进一步研究奠定基础。试验结果证实 ,己烯雌酚及其衍生物存在着顺反异构转化现象 ,酸碱处理是降低交联前衍生物中顺式体比例的有效方法 ;经三步合成 ,成功地制备出了人工抗原 ;人工免疫原及包被原的最适结合比例分别为 1∶2 8和 1∶7。     

小鹅瘟病毒荧光抗体的制备与应用

将小鹅瘟病毒 (GPV )分离毒xw株接种健康兔获得兔抗GPV超免疫血清 ,由此制备兔抗GPV荧光抗体。采用直接荧光抗体试验对人工感染雏鹅、鹅胚及自然病例进行了检测。结果表明 ,该方法可检出患病组织中的病毒抗原 ,且肠和肝的含毒量高于尿囊细胞。     

微小牛蜱幼蜱cDNA表达文库的构建

为了研究微小牛蜱功能性抗原基因,从微小牛蜱(Boophilus microplus)幼蜱中提取总RNA并分离纯化mRNA。采用Oligo(dT)引物合成双链cDNA,在其两端加EcoRⅠ、HindⅢ定向接头,用Mini Column Fractionation凝胶过滤柱纯化后定向克隆到λSCREEN载体。经体外包装,成功构建了我国微小牛蜱幼蜱cDNA表达文库,铺板测定原始库容量为4.5×105PFU,扩增后的滴度为7.2×1010PFU/mL。     

人体不同体液内PSA含量及其法医学意义

目的检测人体不同体液内前列腺特异抗原(PSA)含量,探讨其法医学价值。方法收集成年人(19～63岁)晨尿40份(男28份、女12份)、血液58份(男45份、女13份)、唾液25份(男14份、女11份);青少年(10～15岁)男性晨尿205份;哺乳期(25～31岁)女性乳汁9份;使用Cobas e411型全自动电化学发光免疫分析系统及T-PSA定量测定试剂盒,检测各样本T-PSA含量;分析不同体液及不同年龄青少年男性尿液PSA含量差异。结果除男、女性唾液外,其它样本均可检测到PSA,其中成年男性尿液含量最高,与其它体液比较具有显著性差异(P<0.000 1);青少年男性各年龄组尿液PSA含量随年龄逐年增高,11岁及以下年龄组含量不足1ng/mL,14岁及以上年龄组可超过1 000ng/mL。结论前列腺发育成熟的男性尿液PSA含量较高,在进行精液斑的法医学检验时应给予充分注意。     

旋毛虫ES抗原对小鼠巨噬细胞TLR2/4mRNA表达的影响

为探讨旋毛虫ES抗原对RAW264.7细胞TLR2/4mRNA表达的影响,分别取经0、2、5、15、30、45μg/mL ES抗原作用24h的RAW264.7细胞和用15μg/mL ES抗原作用0、3、6、12、18、24h后的RAW264.7细胞,采用半定量PCR方法检测TLR2和TLR4mRNA的表达水平变化。结果显示,随着ES抗原浓度的升高,TLR2/4mRNA的表达量逐渐上升,15μg/mL ES抗原组与空白对照组相比差异显著(P<0.05)。在15μg/mL ES抗原作用24h内,随着作用时间的延长,TLR2/4mRNA的表达量逐渐上升,作用18h后的表达水平升高,且与空白对照组差异显著(P<0.05)。证实,ES抗原可刺激RAW264.7细胞表面受体TLR2/4表达升高,且存在一定的剂量和时间效应。     

内异止痛方直肠用药对子宫内膜异位症痛经患者血清癌抗原125和血管内皮生长因子的影响

目的 观察内异止痛方直肠用药治疗子宫内膜异位症痛经的临床疗效及其对血清癌抗原125（cancer antigen 125, CA125）、血管内皮生长因子（vascular endothelial growth factor, VEGF）的影响。方法 将60例子宫内膜异位症痛经患者随机分为治疗组与对照组各30例。治疗组予内异止痛方灌肠，对照组予散结镇痛胶囊口服。两组均以15 d为1个疗程，连续治疗3个疗程，随访3个月后评估临床疗效及治疗前后CA125、VEGF的变化。结果 两组治疗后血清CA125、VEGF水平均较治疗前明显下降（P<0.05），治疗组CA125、VEGF水平下降程度明显大于对照组（P<0.05）；治疗组临床疗效优于对照组，差异具有统计学意义（P<0.05）。结论 内异止痛方直肠用药通过降低血清CA125和VEGF水平治疗子宫内膜异位症痛经。     

H5N1和H9N2亚型禽流感病毒型特异性抗原捕捉ELISA检测方法的建立

采用禽流感病毒多克隆抗体及型特异性单克隆抗体,研究建立了型特异性抗原捕捉ELISA检测方法,用于检测H5N1和H9N2亚型禽流感病毒。优化了反应条件,确定了包被抗体、检测抗体及酶结合物的最佳工作浓度,对该方法的敏感性、特异性、重复性及稳定性进行了分析,并与病毒分离鉴定的结果进行了比较。结果表明,该方法敏感、特异,具有良好的重复性和稳定性,可用于临床样品及鸡胚、细胞培养物中禽流感病毒的检测。     

实验性脑挫伤GFAP,PCNA免疫组织化学研究

采用改进的Ｆｅｅｎｅｙ氏落体打击致Ｗｉｓｔａｒ大鼠脑挫伤模型,利用免疫组织化学染色,观察大鼠实验性脑挫伤后ＧＦＡＰ、ＰＣＮＡ的改变,用图像分析仪对免疫组化染色阳性细胞灰度和面积进行测量,ＳＡＳ统计软件分析,得出脑挫伤后ＧＦＡＰ、ＰＣＮＡ变化的时间规律性,为脑挫伤形成时间推断提供新的手段。ＧＦＡＰ阳性细胞面积和灰度伤后呈上升趋势,伤后３ｈ,即有显著增加（Ｐ＜０．０１）,至４天时,阳性细胞灰度,面积达到峰值（Ｐ＜０．０１）,伤后７天仍保持较高水平（Ｐ＜０．０１）。ＰＣＮＡ阳性细胞伤后１２ｈ出现,灰度呈上升趋势,面积呈下降趋势,伤后３天时达到最大值（Ｐ＜０．０１）。因此,ＧＦＡＰ、ＰＣＮＡ免疫组织化学染色阳性细胞的数量,灰度,面积改变有时间性规律;推断２～７天的脑挫伤则以ＧＦＡＰ、ＰＣＮＡ改变为主;脑挫伤后星形胶质细胞数目增多,只有很少一部分为星形胶质细胞增殖而来,且这种增殖在伤后３天时达到高峰