压力循环技术用于人指甲DNA提取及方法学评价

目的将压力循环技术（PCT）用于指甲DNA提取,并对方法学进行评价。方法收集10份人指甲样本,剪碎约为1mm×1mm大小,采用10%漂白粉水,10%SDS,10%漂白粉水,无菌水清洗样本。10份样本各分成两组,1组用压力循环技术处理,另1组不作处理,提取DNA经复合扩增并进行STR分型检测,用于评价压力循环技术的作用。取5份指甲样本用血浸泡,5份用去离子水浸泡,之后采用上述清洗方法各清洗1-3次,收集各次清洗用的无菌水提取DNA,经STR分型检测,用于评价清洗对去除外源性DNA的效果。结果 10份经压力循环技术处理的样本中有7例比相应未经处理样本DNA提取量更高,但两组进行统计学处理,差异不具有统计学意义（P〉0.05）;两组样本中提取DNA含量在0.026 ng以上的样本均得到完整的STR分型,与相应口腔拭子样本对照准确无误。血污染和非血污染样本清洗二次以上,均可避免外源性DNA的污染。结论使用压力循环技术并配合本文清洗方法,可有效提高人指甲DNA的提取效率,并避免外源性生物DNA的干扰,保证DNA分型结果的准确。     

法庭调解语言中的修辞技艺探析——以亚里士多德的古典修辞学思想为线索

亚里士多德的古典修辞学理论认为,修辞是一种在每一事例上发现可行的说服方式的能力,其功能不在于说服,而在于发现存在于每一事例中的说服方式。他认为凭借修辞方法和说话人的努力可以达成的说服论证包括三种:运用说话人的品格(ethos)、激发听众的某种情感(pathos)和运用逻辑论证(logos)。亚里士多德这些技术性的说服修辞技巧以及他关于法庭(司法)演说、议事(或政治)演说和展示性(夸耀性)演说的分类,均可创造性地应用于法庭调解实践当中,具有重要的方法论意义。     

论带犬民警侦查意识的培养

侦查意识是侦查行为的先导,警犬技术应用于侦查破案其效果一定程度取决于带犬民警侦查意识的强弱。当前,警犬技术在刑事案件侦查中广泛应用、带犬民警流血牺牲事件偶发以及犯罪分子反侦查意识增强等,决定了应当加强对带犬民警侦查意识的培养。为提高带犬民警侦查意识,公安机关应建立带犬民警积极参与侦查活动的机制,加强对带犬民警侦查业务知识的培养,善于总结警犬使用过程中成功与失败之经验,并加强带犬民警模拟实战的训练。     

谈监狱审讯口才的表达方法与技巧

审讯又犯罪嫌疑人，是一场场尖锐复杂、面对面攻心斗智的战斗。尽管负责狱内侦查工作的监狱人民警察在审讯中居于主动地位，而审讯对象居于被动地位，但由于监狱人民警察面对的审讯对象一般都具有对付审讯的伎俩，这就决定了其在审讯中必须注重审讯口才表达方法与技巧的运用。文章在概念解释的基础上，详细介绍了提问、应答的口才表达方法与技巧，这对于狱内审讯实践而言，具有一定的指导意义。     

Evaluating probabilistic genotyping for low-pass DNA sequencing

Most genomic methods consider the sample genotype. Data are evaluated at some location, and if the signal strength is sufficient, a genotype call is made. Conversely, sites that lack sufficient signal are treated as missing data. Such methods for genotype calling are binary, and this dichotomy limits genomic analyses to relatively high-coverage (and high-cost) massively parallel sequencing (MPS) data. It follows that bioinformatic methods that rely on genotypes may not be ideal for trace DNA samples, such as those sometimes encountered in forensic investigations, but even when applicable such analyses can be expensive. However, there are some genomic analyses where having many uncertain genotypes (with measured uncertainty) assayed over the entirety of the genome may be more powerful than current multi-locus approaches that consider a limited number of well-characterized markers. Methods for such problems may rely on genotype likelihood, which expresses the likelihood of alternative genotype calls in addition to the most likely call. One application that can benefit from genotype likelihoods is kinship analysis. NgsRelate is a bioinformatic tool that infers pairwise relatedness using a probabilistic genotyping framework, which accommodates the uncertainty associated with genotype calls for low-pass MPS data. Here, NgsRelate was used to infer kinship coefficients from low-pass whole genome sequencing data from a known pedigree. Multiple samples in a titration series (ranging from 50 ng to 0.5 ng) on a single MPS S4 flow cell were assessed. A reproducible scientific bioinformatic workflow was developed to evaluate kinship coefficients considering up to 3rd degree relatives. NgsRelate was found to provide robust assessments of kinship. Further, the use of low-pass MPS data provides a more cost-effective way to conduct forensic investigations.     

Mismatched multiplex PCR amplification and subsequent RFLP analysis to simultaneously identify polymorphisms of erythrocytic ESD, GLO1, and GPT genes

ESD (esterase D), GLO1 (glyoxalase I), and GPT (glutamate pyruvate transaminase) are human erythrocytic isoenzymes and have previously been applied in forensic medicine caseworks. The molecular bases of the polymorphic gene expression products have been demonstrated to be because of SNPs in respective coding regions. However, it has not been revealed whether the SNPs conferring the polymorphisms to the aforementioned erythrocytic isoenzymes could be simultaneously detected by using a simple PCR method. In this study, we used mismatched primers to simultaneously amplify three common isoenzyme loci so that all amplified products contained the same Hph I cleavage sites. The products were then digested with Hph I and electrophoretically separated and stained so that alleles were identified. The accumulated values for the probability of discrimination power and excluding the probability of paternity to the aforementioned systems attained 90.41% and 41.72%, respectively, in the Chinese Han population. This assay could be extremely valuable for future forensic medicine practices.     

Autosomal short tandem repeat analysis of ancient DNA by coupled use of mini- and conventional STR kits

Abstract: Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpF?STR^® MiniFiler? kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpF?STR^® MiniFiler? and Identifiler? kits. Whereas only 21.87% of larger‐sized loci profiles could be obtained with the Identifiler? kit, 75% of the same loci profiles were determined by MiniFiler? kit analysis. This very successful amplification of large‐sized STR markers from highly degraded aDNA suggests that the MiniFiler? kit could be a useful complement to conventional STR kit analysis of ancient samples.     

Complex mixtures: A critical examination of a paper by Homer et al.

DNA evidence in criminal cases may be challenging to interpret if several individuals have contributed to a DNA-mixture. The genetic markers conventionally used for forensic applications may be insufficient to resolve cases where there is a small fraction of DNA (say less than 10%) from some contributors or where there are several (say more than 4) contributors. Recently methods have been proposed that claim to substantially improve on existing approaches [1]. The basic idea is to use high-density single nucleotide polymorphism (SNP) genotyping arrays including as many as 500,000 markers or more and explicitly exploit raw allele intensity measures. It is claimed that trace fractions of less than 0.1% can be reliably detected in mixtures with a large number of contributors. Specific forensic issues pertaining to the amount and quality of DNA are not discussed in the paper and will not be addressed here. Rather our paper critically examines the statistical methods and the validity of the conclusions drawn in Homer et al. (2008) [1].We provide a mathematical argument showing that the suggested statistical approach will give misleading results for important cases. For instance, for a two person mixture an individual contributing less than 33% is expected to be declared a non-contributor. The quoted threshold 33% applies when all relative allele frequencies are 0.5. Simulations confirmed the mathematical findings and also provide results for more complex cases. We specified several scenarios for the number of contributors, the mixing proportions and allele frequencies and simulated as many as 500,000 SNPs.A controlled, blinded experiment was performed using the Illumina GoldenGate^® 360 SNP test panel. Twenty-five mixtures were created from 2 to 5 contributors with proportions ranging from 0.01 to 0.99. The findings were consistent with the mathematical result and the simulations.We conclude that it is not possible to reliably infer the presence of minor contributors to mixtures following the approach suggested in Homer et al. (2008) [1]. The basic problem is that the method fails to account for mixing proportions.     

劳动法在立法技术上的缺陷及其完善

劳动法是劳动关系方面的基本法律,科学而完善劳动法能够规范劳动关系双方当事人的行为,调整劳动关系,促进劳动关系和谐而稳定的发展,促进社会经济的发展,乃至社会的稳定。反之,如果劳动立法不是很健全,不是很科学,势必会影响到劳动关系的调整和劳动关系的稳定。我国劳动法尚需完善。     

直接扩增法用于重大灾难事故中软骨的个体识别

目的探讨直接扩增法对软骨STR检验的有效性,以提高灾难受害者身源鉴定工作效率。方法采用Power Plex~21试剂盒对88份软骨进行直接扩增法检验,同步进行磁珠法比较分型结果。结果 88份软骨检材中,直接扩增法与磁珠法均成功检出84份检材的STR基因型,两者分型结果完全一致。结论Power Plex~21试剂盒直接扩增法可以应用于软骨STR分型检验,且操作简便、快速,在重大灾难事故身源鉴定中具有很好的应用前景。