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目的统计用33.15及33.6探针对中国人进行DNA指纹图检验的群体调查资料,为实际案件的检验提供理论依据。方法应用33.15及33.6探针为中国北京地区无关群体的血液进行DNA指纹图分析。结果应用33.15探针检验15人的DNA指纹图,无关个体间相关机率为1.03×10-15,两无关个体间出现同一谱带的平均概率0.176;应用33.6探针检验19人的DNA指纹图,无关个体间相关机率为1.53×11-11,两无关个体间出现同一港带的平均概率为0.187;两探针均符合孟德尔遗传规律,均具有组织同一性。结论本研究结果可应用于法医物证检验的亲子鉴定及个体识别。 相似文献
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DNA指纹技术在亲子鉴定中的应用 总被引:3,自引:0,他引:3
用非放射性标记α-珠蛋白-3'HVR探针在低强度洗涤条件下,对人的DNA指纹进行了分析,获得了清晰易读的DNA指纹图。并用该方法调查了云南省的78名无关个体,经统计学分析,计算出无关个体的相关机率是2.4×10-4。用该方法进行亲子鉴定,获得了满意的结果,用HinfⅠ和HeaⅢ两种内切酶酶切,解决了子代陌生带问题,用该方法对二十余起亲权纠纷案进行了鉴定,证明该方法稳定、结果可靠,父权肯定机率均在99.9%以上。 相似文献
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应用 MYO DNA 探针对中国人进行了 DNA 遗传指纹图检验。从两个家系16人及100个无关个体中取肘静脉血提取 DNA,用限制性内切酶 HinfⅠ或 HaeⅢ水解,1%琼脂糖凝胶电泳分离,经 Southern印迹转移,MYO DNA 探针杂交,获得了清晰可辨的 DNA 指纹图谱。结果每个个体在3.0Kb 以上均能检出10条以上杂交区带,个体间的相关概率<4×10~(-9),由杂交区带构成的图谱是个体特异的,杂交区带遵循孟德尔的显性遗传方式由亲代向子代遗传;具有 DNA Fingerprints 的特点。对两起亲子鉴定的案例进行指纹图检验,孩子所存在的杂交区带,除来自母亲外,其余可在嫌疑父亲带中找到,肯定了孩子与嫌疑人的父子关系。MYO DNA 探针在亲子鉴定与个人识别的法医学鉴定中有着重要的实用价值。 相似文献
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本文从一些多态位点中筛选出在中国人群中,对于同一种限制酶HaeⅢ酶解都能检出良好多态性的三个单位点探针(PMLJ14、PYNH24、α-globin-3’HVR)。对这三个位点的等位基因频率进行了调查.用DNA指纹自动识别系统进行了数据处理,各位点的数据如下:PMLJ14、杂合度94%,等位基因频率分布0.002~0.051;PYNH24:杂合度89%,等位基因频率分布0.003~0.152;α-globin-3’HVR:杂合度78%,等位基因频率分布0.003~0.077。分析15个家系,未见到变异发生,符合孟德尔遗传规律。这三个位点个人识别中的累加机率是:1.7×10-5~2.1×10-14。 相似文献
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Exclusion of a man charged with murder by DNA fingerprinting 总被引:1,自引:0,他引:1
DNA fingerprinting was used to demonstrate that two murder-rapes committed in 1983 and 1986, respectively, were connected. The probability of chance association of the fingerprint was calculated as 5.8 x 10(-8). The man who had been charged with the murder was excluded because his DNA fingerprint did not match sperm DNA fingerprints obtained from swabs and clothing attributed to the two victims. 相似文献
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Restriction fragment length polymorphism (RFLP) analysis for the purpose of individualization is now being used in casework in the People's Republic of China. This report describes the use of the multilocus minisatellite probe 33.15 to solve three cases, including two homicides and a rape. In the third case, fetal tissue was analyzed to prove that the alleged rapist was, in fact, the father. In each case, analysis of deoxyribonucleic acid (DNA) resulted in a positive match. The probability of chance association of the DNA fingerprint was calculated as 5.6 x 10(-12), which is similar to the figures reported in the literature. 相似文献
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应用‘Myo’小卫星 DNA 探针,Southern 印迹杂交技术,对血斑、精斑、同一个体不同组织进行 DNA 指纹图分析,均获得清晰的图谱。同一个体的血斑与血液、精斑与精液以及不同的组织其 DNA 指纹图谱完全相同。可以根据斑痕或组织与嫌疑个体的血液或某一组织 DNA 的指纹图谱比对以做出同一认定。50μl 血液量的血斑、5μl 精液量的精斑可以获得清晰易辨的指纹图谱。五年的精斑、两年的血斑亦可做出与同源个体新鲜精液、血液完全一致的 DNA 指纹图谱。对杀人、强奸杀人、碎尸等不同案件的血痕、精斑、不同组织碎块进行了 DNA 指纹图检验,均做出了正确的个体认定。本方法的应用为我国法医物证检验提供了新的分析手段,使个体认定得以实现。 相似文献
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汗潜指印的STR分型检测 总被引:11,自引:6,他引:5
目的探索汗潜指印的荧光STR复合扩增检测的方法。方法采用Chelex-100和Microco-100浓缩柱,提取汗潜指印中DNA,STR复合扩增荧光电泳检测。结果 105例汗潜指纹STR分型可明确判读5个以上基因座的占30.3%,个体之间的差异、捺印指印时用力大小以及指印遗留在客体上时间的长短均影响检测成功率。结论该汗潜指印的DNA提取方法步骤简单,方法较为稳定,使单枚汗潜指印可望获得DNA分型。 相似文献
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P M Schneider R Fimmers J Bertrams P Birkner K Braunbeck U Bulnheim M Feuerbach L Henke E Iten E Osterhaus 《Forensic science international》1992,55(1):45-58
A collaborative study using the multilocus minisatellite DNA probe MZ 1.3 was carried out to investigate segregation information, mutation rate, DNA fragment frequencies as well as band sharing characteristics. The fingerprint patterns of 393 children as well as 694 unrelated individuals were analysed after digestion of DNA with the restriction enzyme HinfI. A mutation rate of 1% per meiosis or 0.04% per band was found with a mean number of 26 bands/individual. It was shown that maternal and paternal fragments are inherited in equal proportions. Population frequencies of restriction fragments demonstrated a distribution with increasing frequencies in the small fragment size range below 10 kb as well as the absence of very common or very rare fragments. Our data can be used to calculate simple exclusion probabilities based on the number of non-maternal bands in the child. 相似文献
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B Olaisen B Mev?g R Jonassen G Paulsen E Thorsby P Teisberg 《Zeitschrift für Rechtsmedizin》1987,99(1):55-64
A restriction fragment length polymorphism analysis using double digestion of DNA preparations with XbaI and BglII restriction enzymes and hybridization with C4 and HLA-DR probes is described. The typing conditions selected reveal extensive individual variation in both C4 and DR gene regions. In our panel of 46 unrelated individuals, 37 different phenotypic patterns were recognized when both probes were used, and preliminary discriminative power values of 0.865 and 0.914 were calculated for C4 and DR beta, respectively. The probability of a chance match using both systems is probably about 1.5.10(-2). The potential of this method for individual identification of blood stains was demonstrated on DNA prepared from 6-month-old dried blood stains from seven panel individuals. The seven individuals were all identified when comparing stain DNA patterns with panel control patterns. No RFLP pattern changes were observed following storage of blood stains. Based on these experiments with C4 and DR beta DNA typing under laboratory conditions, it is concluded that DNA typing with such probes may become a powerful tool in future stain identification analyses. 相似文献
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Abigail S. Bathrick Sarah Norsworthy Dane T. Plaza Mallory N. McCormick Donia Slack Robert S. Ramotowski 《Journal of forensic sciences》2022,67(1):149-160
Forensic examiners must determine whether both latent fingerprint development and DNA profiling can be performed on the same area of an evidence item and, if only one is possible, which examination offers the best chance for identification. Latent fingerprints can be enhanced by targeting different components of fingerprint residues with sequential chemical treatments. This study investigated the effects of single-reagent and sequential latent fingerprint development processes on downstream DNA analysis to determine the point at which latent fingerprint development should be stopped to allow for DNA recovery. Latent fingerprints deposited on copy paper by one donor were developed using three sequential processes: 1,8-diazafluoren-9-one (DFO) → ninhydrin → physical developer (PD); 1,2-indanedione-zinc (IND-Zn) → ninhydrin → PD; and IND-Zn → ninhydrin → Oil Red O (ORO) → PD. Samples were examined after the addition of each chemical treatment. DNA was collected with cotton swabs, extracted, quantified, and amplified. DNA yields, peak heights, number of alleles obtained, and percentage of DNA profiles eligible for CODIS upload were examined. DNA profiles were obtained with varying degrees of success, depending on the number and type of treatments used for latent fingerprint development. The treatments that were found to be the least harmful to downstream DNA analysis were IND-Zn and IND-Zn/laser, and the most detrimental treatments were DFO, DFO/laser, and PD. In general, as the number of treatments increase, the opportunities for DNA loss or damage also increase, and it is preferable to use fewer treatments when developing latent fingerprints prior to downstream DNA processing. 相似文献
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In order to test the practical applicability of oligonucleotide fingerprinting in China we have investigated unrelated individuals, family members and a pair of twins from the Beijing area using the probe (CAC)5/(GTG)5. Except for the monozygotic twins highly variable banding patterns were demonstrated as expected for the randomly selected individuals but also for the relatives. On the basis of an initial survey of 50 unrelated individuals the calculated probability for obtaining by chance two identical multilocus patterns is very small (less than 1.93 x 10(-13). Therefore it seems reasonable to conclude that like in caucasians, (CAC)5/(GTG)5 fingerprints are completely individual-specific also in this population. Therefore they have already been used successfully for identification purposes and paternity tests in many actual cases. 相似文献
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Highly polymorphic minisatellite DNA probes. Further evaluation for individual identification and paternity testing 总被引:2,自引:0,他引:2
J C Smith C R Newton A Alves R Anwar D Jenner A F Markham 《Journal - Forensic Science Society》1990,30(1):3-18
Reliable and reproducible protocols have been developed for the routine DNA fingerprinting of individuals using the highly polymorphic minisatellite DNA probes 33.15 and 33.6. Comparison of DNA fingerprinting from 50 individuals has generated further data on the level of band sharing in the DNA fingerprints of unrelated individuals, as well as the number of bands scorable in individuals. These results are consistent with previous studies. The occurrence of mutant bands in offspring has been examined in over 100 families. Further support is presented for the Mendelian inheritance of minisatellite loci and for lack of significant allelism and linkage between different variable DNA fragments detected in a human DNA fingerprint. 相似文献