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排序方式: 共有251条查询结果,搜索用时 15 毫秒
31.
A high selective screening test (Ad-Tip method) for methamphetamine in human urine has been devised. The method involves a brief extraction from a urine sample with an Ad-Tip (ODS-silica minicolumn), washing, eluting with modified Simon's reagent, and coloration with carbonate buffer. Detection limit of methamphetamine in urine is 1 microgram/ml and the test takes within 3 min/sample. The results of the Ad-Tip method were almost identical to those of laboratory analyses. 相似文献
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4个miniSTR基因座复合扩增体系及应用 总被引:1,自引:1,他引:0
目的构建D6S474、D20S482、D4S2408、D6S1017等4个miniSTR基因座复合扩增体系,评价其对腐败检材的应用价值,调查4个基因座在汉族人群中的遗传多态性。方法采用不同荧光标记4个miniSTR基因座上游引物,构建复合扩增体系。用分子克隆方法制备等位基因分型标准物。采用上述体系对135份汉族无关个体血样进行检测,并计算群体遗传学参数。比较该体系与ID试剂盒在降解检材分析中的成功率。结果采用本文复合扩增体系检测,汉族人群中4个基因座基因型频率分布均符合Hardy-Weinberg平衡定律,累积个人识别能力为0.999 666,累积非父排除率为0.914 902。本文体系较ID试剂盒对自然腐败检材的分型成功率更高。结论 4个miniSTR基因座复合扩增体系对法庭科学实践,特别是对腐败检材的检测有应用价值。 相似文献
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目的验证PuriTyperTM纯化试剂盒各项性能指标和法医学应用价值。方法收集及制备抗凝血液、常见案件检材(唾液、烟头、精液、毛发、指甲、骨骼及组织块)、斑痕样本(血斑、唾液斑、精斑)以及模拟添加抑制剂和模仿自然环境中放置的血斑。采用PuriTyperTM纯化试剂盒提取纯化并进行DNA定量,IdentifilerTM复合扩增试剂盒扩增,产物经ABI 3130遗传分析仪进行检测,Genemapper软件分析结果,对该试剂盒灵敏度、稳定性、重复性、检材适应性进行测试。结果采用该试剂盒提取0.1~40μL血液分别获得0.042~26.45ng/μL的DNA。3种斑痕样本DNA产量平行试验结果稳定。不同类型检材重复检验所获IPC的CT平均值在27.60至28.03之间。常见案件检材所得分型与已知结果均一致。结论 PuriTyperTM纯化试剂盒能够满足法医DNA检验的要求,对法医学实践具有重要的应用价值。 相似文献
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Declines in the age at last childbearing in the first demographic transition reflected conscious changes in fertility behaviour during that period, in particular efforts to limit the total number of children. Such fertility limitation behaviour was the net result of ‘cultural causal factors’ on the one hand and ‘structural and economic causal factors’ on the other hand. This paper analyses the evolution of women’s age at last childbearing by reconstructing women’s life histories based on data from the multi-source COR* historical sample for Antwerp in the period 1846–1920. The paper also assesses the causes of this evolution through a number of theoretically grounded structural/economic, cultural and life course determinants, placing these concepts in a macro-micro framework of methodological individualism. For this purpose, in the first place a Kaplan-Meier analysis is applied to 10-year birth cohorts; a proportional hazard model is also applied to three different birth cohorts (mothers born before 1840; born in the period 1840–1859; and born after 1860); and a range of cultural and life course determinants are analysed, including women’s literacy status, marriage witness characteristics, the seasonality of marriages and births, and birth histories. The analysis confirms the decline in the age at last childbearing especially in the late cohort, and also highlights inter-cohort differences caused by cultural and life course determinants. 相似文献
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Romolo Caniglia Elena Fabbri Claudia Greco Marco Galaverni Ettore Randi 《Forensic Science International: Genetics Supplement Series》2010,4(5):334-338
The recent expansion of the Italian wolf population through the Apennine and western Alps, after centuries of contractions, is causing conflicts with human activities leading to a rise in poaching or illegal killings. Here we show how molecular population genetics has been used to identify a suspect serial wolf killer. We analysed DNA extracted from a necklace made of ten presumed wolf canine teeth, confiscated in 2008 to a man living in the northern Italian Apennine (Liguria Region). Individual genotypes were determined using 12 unlinked autosomal microsatellites (STRs), mtDNA control-region sequences, a male-specific ZFX/ZFY restriction-site and three Y-linked STRs. Results indicate that the teeth belonged to six different individuals (three males and three females), which were assigned to the Italian wolf population with p > 0.90 by Bayesian procedures. One of these genotypes matched with the genetic profile of a male wolf previously found-dead and already non-invasively sampled in the same area. Another genotype matched with that of a female wolf non-invasively sampled twice in the same area 1 year before. These data are being used as forensic genetic evidence in the ongoing criminal trial against the suspect serial wolf killer. 相似文献
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Evaluation of Skin Surface as an Alternative Source of Reference DNA Samples: A Pilot Study 下载免费PDF全文
Mohammed H. Albujja M.Sc. Abdul Aziz Bin Dukhyil Ph.D. Abdul Rauf Chaudhary M.Phil. Ahmed Ch. Kassab Ph.D. Ahmed M. Refaat Ph.D. Saranya Ramesh Babu M.Sc. Mohammad K. Okla Ph.D. Sachil Kumar Ph.D. 《Journal of forensic sciences》2018,63(1):227-233
An acceptable area for collecting DNA reference sample is a part of the forensic DNA analysis development. The aim of this study was to evaluate skin surface cells (SSC) as an alternate source of reference DNA sample. From each volunteer (n = 10), six samples from skin surface areas (forearm and fingertips) and two traditional samples (blood and buccal cells) were collected. Genomic DNA was extracted and quantified then genotyped using standard techniques. The highest DNA concentration of SSC samples was collected using the tape/forearm method of collection (2.1 ng/μL). Cotton swabs moistened with ethanol yielded higher quantities of DNA than swabs moistened with salicylic acid, and it gave the highest percentage of full STR profiles (97%). This study supports the use of SSC as a noninvasive sampling technique and as a extremely useful source of DNA reference samples among certain cultures where the use of buccal swabs can be considered socially unacceptable. 相似文献
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目的基于二代测序平台进行混合检材精细化STR分型,并评估其法医学应用价值。方法收集性侵案件中3例混合检材及其比对样本,采用M48磁珠提取纯化试剂盒提取样本DNA,使用Foren SeqTM DNA Signature Prep试剂盒制备文库,Mi Seq FGx平台进行测序,Foren SeqTM Universal Analysis v1.2.1软件进行数据分析,将STR序列多态分型与长度多态分型进行比较。结果对3例混合检材STR分型进行拆分,在D3S1358、D13S317与D9S1122基因座发现存在同一长度多态等位基因包含两个个体的序列多态等位基因的情况。结论二代测序技术可对混合检材进行精细鉴别,为混合分型数据拆分提供更多线索和依据。 相似文献