共查询到18条相似文献,搜索用时 250 毫秒
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汗潜指印的STR分型检测 总被引:11,自引:6,他引:5
目的探索汗潜指印的荧光STR复合扩增检测的方法。方法采用Chelex-100和Microco-100浓缩柱,提取汗潜指印中DNA,STR复合扩增荧光电泳检测。结果 105例汗潜指纹STR分型可明确判读5个以上基因座的占30.3%,个体之间的差异、捺印指印时用力大小以及指印遗留在客体上时间的长短均影响检测成功率。结论该汗潜指印的DNA提取方法步骤简单,方法较为稳定,使单枚汗潜指印可望获得DNA分型。 相似文献
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短波紫外照射对汗潜手印DNA检测的影响初探 总被引:1,自引:0,他引:1
目的探测短波紫外灯的照射是否会对汗潜手印DNA检测产生影响。方法由每名志愿者在纸张上捺印4枚拇指指印使每枚指印的脱落细胞量保持基本相同,抽取每名志愿者所捺印的一枚指印作为一组,共有四组,将三组指印置于短波紫外灯的照射下,照射时间分别设置为10min﹑30min和1h,还有一组不照射,然后用磁珠法对所有指印提取DNA并进行定量。结果短波紫外灯的照射会对汗潜手印中的DNA造成减损,照射时间越长,减损得越多。结论尽量减少短波紫外灯对汗潜手印照射的时间(可控制在10min内),以保证汗潜手印有足够量的DNA而能被用于STR分型检测。 相似文献
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一些案件中的案犯为了掩盖犯罪事实,常会将在现场使用过的物品用水进行冲洗浸泡,而经过水浸泡的客体上指印往往很难显现提取。笔者在实践中利用胶带粘面显现剂对水浸泡过的光滑客体上汗潜指印显现有很好的效果,介绍如下。 相似文献
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汗潜指印DNA提取方法的初步研究 总被引:15,自引:3,他引:12
目的建立渗透性载体及非渗透性载体上汗潜指印DNA的提取和检验方法。方法采用C-有-柱法及SiO2法两种DNA提取方法,PCR扩增后310型遗传分析仪检测。结果载玻片上3枚汗潜指印采用2种方法均可扩增出Amel及9个STR位点;纸上3枚汗潜指印用SiO2法检见Amel及9个STR位点,而用C-有-柱法检验结果不稳定。渗透性及非渗透性载体上1及2枚汗潜指印,采用上述两种DNA提取方法,检验结果均不稳定。结论所建立的方法可以检见渗透性及非渗透性载体上汗潜指印DNA,并达到同一认定的程度。 相似文献
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An exhibit that is often received for examination in cases of robbery or terrorist activity is adhesive tape. This type of exhibit can often, but not always, be successfully processed for fingerprints. The question arises whether or not it is possible to extract and type DNA after the tape has been sequentially processed for fingerprints. In this work, various donors left fingerprints on the adhesive side of tapes. The tapes were then sequentially processed for fingerprints using an alternate light source, cyanoacrylate fuming, and staining with BY-40 and then crystal violet. DNA was subsequently successfully extracted, amplified and typed for six STR loci. 相似文献
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An Optimized DNA Analysis Workflow for the Sampling,Extraction, and Concentration of DNA obtained from Archived Latent Fingerprints 下载免费PDF全文
April D. Solomon M.S. Madison E. Hytinen B.S. Aryn M. McClain M.S. Marilyn T. Miller Ed.D. Tracey Dawson Cruz Ph.D. 《Journal of forensic sciences》2018,63(1):47-57
DNA profiles have been obtained from fingerprints, but there is limited knowledge regarding DNA analysis from archived latent fingerprints—touch DNA “sandwiched” between adhesive and paper. Thus, this study sought to comparatively analyze a variety of collection and analytical methods in an effort to seek an optimized workflow for this specific sample type. Untreated and treated archived latent fingerprints were utilized to compare different biological sampling techniques, swab diluents, DNA extraction systems, DNA concentration practices, and post‐amplification purification methods. Archived latent fingerprints disassembled and sampled via direct cutting, followed by DNA extracted using the QIAamp® DNA Investigator Kit, and concentration with Centri‐Sep? columns increased the odds of obtaining an STR profile. Using the recommended DNA workflow, 9 of the 10 samples provided STR profiles, which included 7–100% of the expected STR alleles and two full profiles. Thus, with carefully selected procedures, archived latent fingerprints can be a viable DNA source for criminal investigations including cold/postconviction cases. 相似文献
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掺杂Cd的水溶性荧光ZnSe/MPA量子点显现胶带手印方法 总被引:1,自引:0,他引:1
目的探索水溶性荧光量子点溶液——掺杂镉的硒化锌(ZnxCd(1-x)Se)对胶带手印的显现方法。方法以巯基丙酸(MPA)为修饰剂合成了掺杂Cd的水溶性荧光ZnSe/MPA量子点,利用它显现水浸胶带及粘连胶带上的油汗指印,并在365nm下拍照成像。结果良好的显现出水浸胶带上的指印以及粘连胶带上的指印。与常规方法相比,优势更为明显。结论水溶性荧光量子点ZnxCd(1-x)Se溶液显出的指印纹线细腻流畅,在法庭科学领域具有很好的应用前景。 相似文献
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目的探究布料载体上潜掌纹经真空镀膜显现后,掌纹DNA提取相关影响因素与检出基因座数目的关系。方法分别进行出汗手与无汗手掌纹按压,放置不同时间;对提取的掌纹DNA进行定量检测;比较白色与黑色布料载体基因座数目检出情况。结果掌纹基因座数目检出随时间延长而递减,出汗手组基因座数目检出高于无汗手组,rfu值600时,基因座数目检出百分比90%,模板量阈值为0.013ng。同一提取方法白布基因座数目检出高于黑布,布料色素抑制PCR扩增,致使基因座检出数目减少。结论真空镀膜技术能够很好地应于掌纹DNA的检测。 相似文献
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福尔马林固定石蜡包埋组织3种DNA提取方法比较 总被引:1,自引:1,他引:0
目的探讨经福尔马林固定1d石蜡包埋组织(FFPET)提取DNA的简易有效方法。方法比较水浴加热、微波加热和二甲苯脱蜡的效果。组织脱蜡后分别采用Chelex-100+层析柱纯化法、DNA IQTM试剂盒磁珠提取法和Chelex-100+磁珠纯化法提取DNA;实时荧光定量PCR技术定量DNA;荧光标记毛细管电泳技术进行STR分型。结果二甲苯脱蜡的效果好于其他两种加热的脱蜡方法(P<0.05)。Chelex-100+层析柱纯化所获得的DNA量显著高于其他两种方法(P<0.05)。结论二甲苯脱蜡、Chelex-100+层析柱纯化法是一种简单、有效的FFPET处理方法。 相似文献
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The spectroscopic detection of exogenous material in fingerprints after development with powders and recovery with adhesive lifters 总被引:1,自引:0,他引:1
The application of powders to fingerprints has long been established as an effective and reliable method for developing latent fingerprints. The powders adhere to the ridge pattern of the fingerprint only, thus allowing the image to be visualised. Fingerprints developed in situ at a crime scene routinely undergo lifting with specialist tapes to facilitate subsequent laboratory analysis. As with all recovered evidence these samples would be stored in evidence bags to allow secure transit from the scene to the laboratory and also to preserve the chain of evidence. In this paper, the application of Raman spectroscopy for the analysis of exogenous material in latent fingerprints is reported for contaminated fingerprints that had been treated with powders and also subsequently lifted with adhesive tapes. A selection of over the counter (OTC) analgesics were used as samples for the analysis and contaminated fingerprints were deposited on clean glass slides. The application of aluminium or iron based powders to contaminated fingerprints did not interfere with the Raman spectra obtained for the contaminants. In most cases background fluorescence attributed to the sebaceous content of the latent fingerprint was reduced by the application of the powder thus reducing spectral interference. Contaminated fingerprints developed with powders and then lifted with lifting tapes were also examined. The combination of these two techniques did not interfere with the successful analysis of exogenous contaminants by Raman spectroscopy. The lifting process was repeated using hinge lifters. As the hinge lifters exhibited strong Raman bands the spectroscopic analysis was more complex and an increase in the number of exposures to the detector allowed for improved clarification. Raman spectra of developed and lifted fingerprints recorded through evidence bags were obtained and it was found that the detection process was not compromised in any way. Although the application of powders did not interfere with the detection process the time taken to locate the contaminant was increased due to the physical presence of more material within the fingerprint. The presence of interfering Raman bands from lifting tapes is another potential complication. This, however, could be removed by spectral subtraction or by the choice of lifting tapes that have only weak Raman bands. 相似文献